Mouse IgG2a, kappa isotype control (DyLight 488)
FACS, WB, ICC, IHC (fro), IHC (p)
Monoclonal
MOPC-173
IgG2a kappa
DyLight 488
1 image
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- Target See all IgG2a, kappa products
- IgG2a, kappa
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Host
- Mouse
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Clonality
- Monoclonal
- Conjugate
- DyLight 488
- Application
- Flow Cytometry (FACS), Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Specificity
- This mouse IgG2a monoclonal antibody (clone MOPC-173) reacts with an unknown epitope. It does not react with a variety of resting, activated, live, and fixed mouse, rat and human tissues.
- No Cross-Reactivity
- Human, Mouse, Rat
- Purification
- Purified antibody is conjugated with DyLight® 488 NHS ester under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
- Immunogen
- The transplantable plasmacytoma MOPC-173 was induced by intraperitoneal injection of mineral oils into BALB/c mice.
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Clone
- MOPC-173
- Isotype
- IgG2a kappa
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Mouse IgG2a, kappa isotype control (DyLight 650)
FACS, WB, ICC, IHC (fro), IHC (p) Monoclonal MOPC-173 IgG2a kappa DyLight 650
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- Application Notes
- Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 μg/mL of incubation mixture), change the conditions of your experiment to reduce the background.
- Restrictions
- For Research Use only
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- Concentration
- 0.1 mg/mL
- Buffer
- Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
- Storage Comment
- Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
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- Target
- IgG2a, kappa
- Alternative Name
- IgG2a kappa (IgG2a, kappa Products)
- Target Type
- Antibody
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