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|Antigen||Ciliary Neurotrophic Factor (CNTF) Antibodies|
|Conjugate||This CNTF antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Functional Studies (Func), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
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Product Details anti-CNTF AntibodyTarget Details CNTF Application Details Handling Images
|Specificity||Ciliary Neurotrophic Factor (CNTF)|
|Immunogen||Highly pure (>98%) recombinant hCNTF.|
Target Details CNTFProduct Details anti-CNTF Antibody Application Details Handling Images back to top
|Alternative Name||CNTF (CNTF Antibody Abstract)|
|Background||Ciliary neurotrophic factor (CNTF) is expressed in glial cells within the central and peripheral nervous systems. CNTF stimulates gene expression, cell survival or differentiation in a variety of neuronal cell types such as sensory, sympathetic, ciliary and motor neurons. CNTF itself lacks a classical signal peptide sequence of a secreted protein, but is thought to convey its cytoprotective effects after release from adult glial cells by some mechanism induced by injury. In addition to its neuronal actions, CNTF also acts on non neuronal cells such as glia, hepatocytes, skeletal muscle, embryonic stem cells and bone marrow stromal cells.Synonyms: Ciliary Neurotrophic Factor|
|Research Area||Stem Cells|
Application DetailsProduct Details anti-CNTF Antibody Target Details CNTF Handling Images back to top
Neutralization. ELISA: To detect hCNTF by direct ELISA (using 100 l/well antibody solution) aconcentration of at least 0.5 μg/mL of this antibody is required. This antigen affinitypurified antibody, in conjunction with compatible secondary reagents, allows the detectionof 0.2-0.4 ng/well of recombinant hCNTF. Western Blot: To detect hCNTF by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hCNTF is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-CNTF Antibody Target Details CNTF Application Details Images back to top
|Reconstitution||Restore in sterile water to a concentration of 0.1-1.0 mg/mL.|
|Buffer||PBS, pH 7.2 without preservatives|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody at 2-8 °C or in aliquots at -20 °C long term.|