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|Application / Reactivity||Rat (Rattus)||Human|
|Blocking Antibody (Inhibition)||3 Antibodies||2 Antibodies|
|Enzyme Immunoassay (EIA)||5 Antibodies|
|Functional Studies (Func)||5 Antibodies|
|Immunochromatography (IC)||1 Antibodies|
|Immunocytochemistry (ICC)||2 Antibodies|
|Immunofluorescence (IF)||3 Antibodies|
|Immunofluorescence (fixed cells) (IF/ICC)||1 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||13 Antibodies|
|Immunohistochemistry (IHC)||17 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||4 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||12 Antibodies|
|Immunoprecipitation (IP)||2 Antibodies|
|Neutralization (Neut)||12 Antibodies|
|Western Blotting (WB)||66 Antibodies|
|Blocking Peptide (BP)||1 Antibodies|
|Cellular Assay (CA)||1 Antibodies|
|Dot Blot (DB)||1 Antibodies|
|Antigen||Ciliary Neurotrophic Factor (CNTF) Antibodies|
|Epitope||AA 2-200 Alternatives|
|Reactivity||Human, Rat (Rattus) Alternatives|
|Conjugate||This CNTF antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|Supplier||Log in to see|
Product Details anti-CNTF AntibodyTarget Details CNTF Application Details Handling Images
|Purpose||Rabbit IgG polyclonal antibody for Ciliary neurotrophic factor(CNTF) detection. Tested with WB, IHC-P in Human,Rat.|
|Cross-Reactivity (Details)||No cross reactivity with other proteins.|
Rabbit IgG polyclonal antibody for Ciliary neurotrophic factor(CNTF) detection. Tested with WB, IHC-P in Human,Rat.
Gene Name: ciliary neurotrophic factor
Protein Name: Ciliary neurotrophic factor
|Purification||Immunogen affinity purified.|
|Immunogen||E.coli-derived human CNTF recombinant protein (Position: A2-M200). Human CNTF shares 83% and 84% amino acid (aa) sequence identity with mouse and rat CNTF, respectively.|
Target Details CNTFProduct Details anti-CNTF Antibody Application Details Handling Images back to top
|Alternative Name||CNTF (CNTF Antibody Abstract)|
Ciliary neurotrophic factor (CNTF) is a potent polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes survival, neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. The CNTF gene is located on chromosome 11, as determined using human-hamster somatic cell hybrids. The CNTF protein is highly conserved in evolution. The amino acid (aa) sequences of rat and rabbit CNTF translated from cDNAs display approx. 85 % homology with the deduced aa sequence encoding human CNTF. CNTF induces weight loss and improves glucose tolerance in humans and rodents. CNTF is thought to act centrally by inducing hypothalamic neurogenesis to modulate food intake and peripherally by altering hepatic gene expression, in a manner similar to that of leptin.
Synonyms: Ciliary Neurotrophic Factor antibody|CNTF antibody|CNTF_HUMAN antibody|HCNTF antibody
|Research Area||Stem Cells|
Application DetailsProduct Details anti-CNTF Antibody Target Details CNTF Handling Images back to top
WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, The detection limit for CNTF is approximately 0.25 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
|Restrictions||For Research Use only|
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|Reconstitution||Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.|
|Buffer||Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
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