anti-Rat (Rattus) CNTF antibody for Enzyme Immunoassay

Recommended CNTF Antibody (supplied by: Log in to see )

Antigen
Ciliary Neurotrophic Factor (CNTF) Antibodies
  • CNTF
  • HCNTF
  • AI429687
  • GPA
  • ciliary neurotrophic factor
  • ciliary neurotrophic factor-like
  • ZFP91 zinc finger protein
  • CNTF
  • Cntf
  • LOC100885759
  • LOC100518821
  • ZFP91
Reactivity
Rat (Rattus)
179
80
51
3
2
2
1
1
1
Host
Rabbit
187
23
17
3
3
Clonality
Polyclonal
Conjugate
This CNTF antibody is conjugated to Biotin
32
14
9
4
4
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
183
127
48
23
14
13
13
9
9
8
7
7
5
4
4
3
2
1
1
1
1
1
Supplier
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Catalog No. ABIN115935
$ 286.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN115936 EIA WB Biotin Rabbit Log in to see Polyclonal
1 ABIN5554093 EIA WB Goat Internal Region Log in to see Polyclonal
1 ABIN115937 EIA Func IHC (fro) WB Rabbit Log in to see Polyclonal
1 ABIN115938 EIA Func IHC (fro) WB Rabbit Log in to see Polyclonal

Similar anti-CNTF Antibodies

Application / Reactivity Rat (Rattus)
Blocking Antibody (Inhibition) 3 Antibodies
ELISA 42 Antibodies
Enzyme Immunoassay (EIA) 5 Antibodies
Functional Studies (Func) 5 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 2 Antibodies
Immunofluorescence (IF) 3 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 13 Antibodies
Immunohistochemistry (IHC) 17 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 4 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 12 Antibodies
Immunoprecipitation (IP) 2 Antibodies
Neutralization (Neut) 12 Antibodies
Western Blotting (WB) 66 Antibodies

General

Antigen Ciliary Neurotrophic Factor (CNTF) Antibodies
Reactivity Rat (Rattus)
(179), (80), (51), (3), (2), (2), (1), (1), (1)
Host Rabbit
(187), (23), (17), (3), (3)
Clonality
Polyclonal
Conjugate This CNTF antibody is conjugated to Biotin
(32), (14), (9), (4), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Western Blotting (WB)
(183), (127), (48), (23), (14), (13), (13), (9), (9), (8), (7), (7), (5), (4), (4), (3), (2), (1), (1), (1), (1), (1)
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Product Details anti-CNTF Antibody

Target Details CNTF Application Details Handling Images
Specificity This antibody recognises Rat CNTF.
Purification Affinity Chromatography.
Immunogen Highly purified recombinant CNTF.

Target Details CNTF

Product Details anti-CNTF Antibody Application Details Handling Images back to top
Antigen
Alternative Name CNTF (CNTF Antibody Abstract)
Background CNTF is a potent neural factor that was originally characterized as a vital factor for the survival of chick ciliary neurons in vitro. CNTF is also important for the survival of other neural cell types including primary sensory neurons, motor neurons, basal forebrain neurons and type 2 astrocytes. CNTF is highly conserved across species and exhibits cross-species bioactivity.Synonyms: Ciliary Neurotrophic Factor
Gene ID 25707
NCBI Accession NP_037298
UniProt P20294
Research Area Stem Cells
Pathways JAK-STAT Signaling

Application Details

Product Details anti-CNTF Antibody Target Details CNTF Handling Images back to top
Application Notes ELISADirect: To detect Rat CNTF by indirect ELISA (using 100 ml/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with compatible secondary reagents, allows the detection of atleast 0.2-0.4 ng/well of recombinant Rat CNTF. Sandwich: To detect Rat CNTF by sandwich ELISA (using 100 ml/well antibody solution) aconcentration of 0.25-1.0 μg/mL of this antibody is required. The Biotinylated antibody inconjunction with Anti-Rat CNTF (PP030P1 or PP030P2) as a Capture antibody, allows thedetection of at least 0.2-0.4 ng/well of recombinant Rat CNTF. Western BlotTo detect Rat CNTF by Western Blot analysis this antibody can be used at a concentration of0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limitfor recombinant Rat CNTF is 1.5-3.0 ng/lane, under either reducing or non-reducingconditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-CNTF Antibody Target Details CNTF Application Details Images back to top
Reconstitution Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.
Buffer PBS, pH 7.2 without preservatives.
Preservative Without preservative
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.