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anti-Human CSF2 antibody for Immunoprecipitation

Recommended CSF2 Antibody (supplied by: Log in to see )

Antigen
Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) Antibodies
  • CSF
  • CSF2
  • Csfgm
  • Gm-csf
  • Gm-CSf
  • GM-CSF
  • GMCSF
  • Gmcsf
  • MGI-IGM
Reactivity
Human
312
115
20
14
10
4
4
2
2
Host
Rat
190
131
121
10
3
Clonality
Monoclonal
Conjugate
This CSF2 antibody is un-conjugated
57
14
13
11
7
5
4
2
1
1
1
1
1
1
1
1
1
Application
Blocking Antibody (Inhibition), Immunoprecipitation (IP), Western Blotting (WB)
285
271
69
66
56
35
34
25
20
19
18
12
9
6
6
5
4
3
3
2
2
2
1
1
1
1
1
1
1
1
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Catalog No. ABIN967465
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00089235755 ABIN2689522 FACS IP ELISA WB Biotin Rat IgG2a Log in to see BVD2 4
0.00089235755 ABIN2689523 IP Neut ELISA WB Rat IgG2a Log in to see BVD2 3
0.00089235755 ABIN2275848 FACS IF IP IHC WB Rat IgG2a kappa Log in to see BVD2-21C11
0.00089235755 ABIN375506 IP ELISA WB Rat IgG2a Log in to see BVD2-23B6
0.00089235755 ABIN375620 IP ELISA WB Rat IgG2a Log in to see BVD2-23B6
0.00089235755 ABIN3024890 FACS IF IP Neut IHC WB Rat IgG2a, kappa Log in to see BVD2-21C11
0.00089235755 ABIN1002369 IP ELISA WB Mouse IgG Log in to see r-Human GM-CSF
0.00089235755 ABIN957627 IP ELISA WB Rat IgG2a, kappa Log in to see BVD2-23B6
0.00089235755 ABIN2276756 IP Neut ELISA WB Rat IgG2a kappa Log in to see 5K117
0.00089235755 ABIN2276755 IP ELISA WB Rat IgG2a kappa Log in to see 5K117
0.00089235755 ABIN2164948 IP ELISA WB Rat IgG2a Log in to see
0.00089235755 ABIN2276754 IP ELISA WB Rat IgG2a Log in to see 10B2332
0.00089235755 ABIN3024891 FACS IF IP Neut IHC WB Rat IgG2a, kappa Log in to see BVD2-21C11
0.00089235755 ABIN2142610 IP ELISA WB Mouse IgG Log in to see r-Human GM-CSF
0.00089235755 ABIN3024892 IHC (fro) FACS IF IP Neut WB Rat IgG2a, kappa Log in to see SPM469
0.00089235755 ABIN217534 IP ELISA WB Rat IgG2a Log in to see
0.00089235755 ABIN2437714 IP ELISA WB Mouse IgG Log in to see NYRhGMCSF-
0.00089235755 ABIN3024893 IHC (fro) FACS IF IP Neut WB Rat IgG2a, kappa Log in to see SPM469
0.00089235755 ABIN2473857 IP ELISA WB Rat IgG2a Log in to see BVD2-23B6
0.00089235755 ABIN808128 IP TCC ELISA Mouse Log in to see NYRhGMCSF

Top referenced anti-CSF2 antibody for Immunoprecipitation

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Application / Reactivity Human
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ELISA 183 Antibodies
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ELISA (Detection) 3 Antibodies
ELISpot 1 Antibodies
Enzyme Immunoassay (EIA) 24 Antibodies
Flow Cytometry (FACS) 51 Antibodies
Functional Studies (Func) 11 Antibodies
Immunoassay (IA) 3 Antibodies
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Immunohistochemistry (Frozen Sections) (IHC (fro)) 10 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 12 Antibodies

General

Antigen Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) Antibodies
Reactivity Human
(312), (115), (20), (14), (10), (4), (4), (2), (2)
Host Rat
(190), (131), (121), (10), (3)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CSF2 antibody is un-conjugated
(57), (14), (13), (11), (7), (5), (4), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Blocking Antibody (Inhibition), Immunoprecipitation (IP), Western Blotting (WB)
(285), (271), (69), (66), (56), (35), (34), (25), (20), (19), (18), (12), (9), (6), (6), (5), (4), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 6 references available
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Product Details anti-CSF2 Antibody

Target Details CSF2 Application Details Handling References for anti-CSF2 Antibody (ABIN967465) Images
Brand BD Pharmingen™
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Ficoll-Paque is a trademark of Amersham Biosciences Limited.
5. Cy is a trademark of Amersham Biosciences Limited.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Recombinant human GM-CSF
Clone BVD2-21C11
Isotype IgG2a

Target Details CSF2

Product Details anti-CSF2 Antibody Application Details Handling References for anti-CSF2 Antibody (ABIN967465) Images back to top
Antigen
Alternative Name GM-CSF (CSF2 Antibody Abstract)
Background The BVD2-21C11 monoclonal antibody specifically binds to human Granulocyte/Macrophage - Colony Stimulating Factor (GM-CSF). Human GM-CSF is encoded by the CSF2 gene and is also known as Colony Stimulating Factor 2. GM-CSF is produced by activated T lymphocytes, macrophages, endothelial cells, fibroblasts, stromal cells and other cell types including B lymphocytes, mast cells, eosinophils, and osteoblasts. GM-CSF stimulates the survival, proliferation and/or differentiation of various cell types including neutrophils, eosinophils, macrophages, dendritic cells, megakaryocytes, erythroid cells, endothelial cells and their precursors. The immunogen used to generate the BVD2-21C11 hybridoma was recombinant human GM-CSF. The BVD2-21C11 antibody has been reported to crossreact with GM-CSF from the rhesus monkey. BVD2-21C11 is a neutralizing antibody.
Synonyms: CSF2, Colony stimulating factor 2 (granulocyte-macrophage), CSF, GMCSF
Research Area Immunology, Innate Immunity, Cytokines
Pathways JAK-STAT Signaling

Application Details

Product Details anti-CSF2 Antibody Target Details CSF2 Handling References for anti-CSF2 Antibody (ABIN967465) Images back to top
Application Notes Blocking Control for Intracellular Staining:
The purified BVD2-21C11 antibody (ABIN967465) can be used as a blocking control to demonstrate specificity of human GM-CSF staining by the PE-BVD2-21C11. To perform this control, the fixed/permeabilized cells (~ 1 million) can be incubated with 1 - 10 µg of unlabeled BVD2-21C11 antibody (ABIN967465) for 20 minutes at 4°C, prior to staining with PE-BVD2-21C11 antibody (eg, 0.1 - 0.5 µg mAb/ 1 million cells). The intracellular cytokine staining technique and use of blocking controls are described in detail by C. Prussin and D. Metcalfe.

ELISA Detection:
The biotinylated BVD2-21C11 antibody, is useful as a detection antibody for a sandwich ELISA for measuring human GM-CSF protein levels. Biotinylated BVD2-21C11 antibody can be paired with the purified BVD2-23B6 antibody as the capture antibody, with recombinant human GM-CSF as the standard. This ELISA pair is recommended primarily for measuring cytokine from experimental cell culture systems.
Restrictions For Research Use only

Handling

Product Details anti-CSF2 Antibody Target Details CSF2 Application Details References for anti-CSF2 Antibody (ABIN967465) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C.

References for anti-CSF2 Antibody (ABIN967465)

Product Details anti-CSF2 Antibody Target Details CSF2 Application Details Handling Images back to top
Product cited in:

Abrams: "Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies." in: Current protocols in immunology / edited by John E. Coligan ... [et al.], Vol. Chapter 6, pp. Unit 6.20, 2008

Prussin, Metcalfe: "Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies." in: Journal of immunological methods, Vol. 188, Issue 1, pp. 117-28, 1996

Abrams, Roncarolo, Yssel, Andersson, Gleich, Silver: "Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples." in: Immunological reviews, Vol. 127, Issue 9-10, pp. 5-24, 1992

Kita, Ohnishi, Okubo, Weiler, Abrams, Gleich: "Granulocyte/macrophage colony-stimulating factor and interleukin 3 release from human peripheral blood eosinophils and neutrophils." in: The Journal of experimental medicine, Vol. 174, Issue 3, pp. 745-8, 1991

Bacchetta, de Waal Malefijt, Yssel, Abrams, de Vries, Spits, Roncarolo: "Host-reactive CD4+ and CD8+ T cell clones isolated from a human chimera produce IL-5, IL-2, IFN-gamma and granulocyte/macrophage-colony-stimulating factor but not IL-4." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 144, Issue 3, pp. 902-8, 1990

Background publications

Masaki, Kano, Merrick, Milgrom: "Studies on Paul-Bunnell (P-B) antigen-antibody system. II. P-B antigens in extracts of lymphoma-leukemia spleens and pathologic sera." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 127, Issue 1, pp. 5-8, 1981

Images

Product Details anti-CSF2 Antibody Target Details CSF2 Application Details Handling References for anti-CSF2 Antibody (ABIN967465) back to top
Supplier Images
 image for anti-CSF2 antibody (Colony Stimulating Factor 2 (Granulocyte-Macrophage)) (ABIN967465) Expression of GM-CSF by stimulated human peripheral blood mononuclear cells (PBMC). F...
 image for anti-CSF2 antibody (Colony Stimulating Factor 2 (Granulocyte-Macrophage)) (ABIN967465) Preincubation of the antibody conjugate with recombinant human GM-CSF
 image for anti-CSF2 antibody (Colony Stimulating Factor 2 (Granulocyte-Macrophage)) (ABIN967465) Preincubation of the fixed/permeabilized cells with unlabeled BVD2-21C11 antibody