anti-Rabbit EPO antibody for Western Blotting

Recommended EPO Antibody (supplied by: Log in to see )

Antigen
Erythropoietin (EPO) Antibodies
  • EPO
  • EP
  • MVCD2
  • erythropoietin
  • EPO
  • epo
  • Epo
Epitope
Middle Region
19
15
15
14
13
13
8
6
6
5
5
3
3
3
3
3
2
2
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Sheep (Ovine)
337
75
42
7
4
2
2
2
1
1
1
1
Host
Rabbit
189
172
15
5
3
1
Clonality
Polyclonal
Conjugate
This EPO antibody is un-conjugated
28
21
16
10
6
6
6
5
5
5
5
5
5
5
5
4
4
4
4
3
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
250
216
67
60
57
52
31
13
11
11
7
6
4
3
2
2
2
2
2
1
1
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Catalog No. ABIN2781825
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN574986 WB Rabbit C-Term Log in to see Polyclonal
1 ABIN379921 WB Chicken Log in to see Polyclonal

General

Antigen Erythropoietin (EPO) Antibodies
Epitope Middle Region
(19), (15), (15), (14), (13), (13), (8), (6), (6), (5), (5), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Sheep (Ovine)
(337), (75), (42), (7), (4), (2), (2), (2), (1), (1), (1), (1)
Host Rabbit
(189), (172), (15), (5), (3), (1)
Clonality
Polyclonal
Conjugate This EPO antibody is un-conjugated
(28), (21), (16), (10), (6), (6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (4), (4), (4), (4), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Western Blotting (WB)
(250), (216), (67), (60), (57), (52), (31), (13), (11), (11), (7), (6), (4), (3), (2), (2), (2), (2), (2), (1), (1)
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Product Details anti-EPO Antibody

Target Details EPO Application Details Handling Images
Blocking Peptide Blocking peptide for this product available: ABIN976390
Sequence KEAISPPDAA SAAPLRTITA DTFRKLFRVY SNFLRGKLKL YTGEACRTGD
Predicted Reactivity Cow: 93%, Dog: 93%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Sheep: 93%
Characteristics This is a rabbit polyclonal antibody against EPO. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the middle region of human EPO

Target Details EPO

Product Details anti-EPO Antibody Application Details Handling Images back to top
Antigen
Alternative Name EPO (EPO Antibody Abstract)
Background This gene is a member of the EPO/TPO family and encodes a secreted, glycosylated cytokine composed of four alpha helical bundles. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. This protein also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types.
Alias Symbols: EP, MGC138142, MVCD2
Protein Interaction Partner: EP300, EPOR, SIM2, RB1, HIF1A,
Protein Size: 193
Molecular Weight 18 kDa
Gene ID 2056
NCBI Accession NM_000799, NP_000790
UniProt P01588
Research Area Cancer, Organogenesis, Hematopoietic Progenitors, Angiogenesis
Pathways JAK-STAT Signaling, Hormone Activity, Negative Regulation of intrinsic apoptotic Signaling, Negative Regulation of Transporter Activity

Application Details

Product Details anti-EPO Antibody Target Details EPO Handling Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 193 AA

Restrictions For Research Use only

Handling

Product Details anti-EPO Antibody Target Details EPO Application Details Images back to top
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Images

Product Details anti-EPO Antibody Target Details EPO Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-Erythropoietin (EPO) (Middle Region) antibody (ABIN2781825) WB Suggested Anti-EPO Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1:312500 Positive ...