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anti-Human IL-4 antibody for ELISpot (Capture)

Recommended IL-4 Antibody (supplied by: Log in to see )

Antigen
Interleukin 4 (IL4) Antibodies
  • BCGF-1
  • BCGF1
  • BSF-1
  • BSF1
  • IL-4
  • Il-4
  • IL2
  • IL4
  • Il4e12
Reactivity
Human
328
182
50
25
19
15
7
7
7
6
6
5
4
3
3
3
2
2
1
Host
Mouse
244
200
151
8
7
2
Clonality
Monoclonal
Conjugate
This IL-4 antibody is un-conjugated
90
47
31
23
18
8
7
6
4
4
4
2
1
1
1
1
Application
ELISA (Capture), ELISpot (Capture) (ELISPOT (Capture))
341
304
187
84
56
31
28
27
24
23
20
20
20
18
10
6
5
5
3
3
2
2
2
2
2
1
1
1
1
1
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Catalog No. ABIN281621
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008676689 ABIN1722427 ELISA (Capture) ELISPOT (Capture) Mouse IgG1 Log in to see B-R14

Top referenced anti-IL-4 antibody for ELISpot (Capture)

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General

Antigen Interleukin 4 (IL4) Antibodies
Reactivity Human
(328), (182), (50), (25), (19), (15), (7), (7), (7), (6), (6), (5), (4), (3), (3), (3), (2), (2), (1)
Host Mouse
(244), (200), (151), (8), (7), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This IL-4 antibody is un-conjugated
(90), (47), (31), (23), (18), (8), (7), (6), (4), (4), (4), (2), (1), (1), (1), (1)
Application ELISA (Capture), ELISpot (Capture) (ELISPOT (Capture))
(341), (304), (187), (84), (56), (31), (28), (27), (24), (23), (20), (20), (20), (18), (10), (6), (5), (5), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-IL-4 Antibody

Target Details IL-4 Application Details Handling References for anti-IL-4 Antibody (ABIN281621) Images
Specificity The 8D4-8 antibody reacts with human interleukin-4 (IL-4), a 15-19 kDa cytokine secreted by Th2 cells.
Characteristics The 8D4-8 antibody reacts with human interleukin-4 (IL-4), a 15-19 kDa cytokine secreted by Th2 cells.
Synonyms: Synonyms: Interleukin-4
Purification Affinity Purified
Clone 8D4-8
Isotype IgG1 kappa

Target Details IL-4

Product Details anti-IL-4 Antibody Application Details Handling References for anti-IL-4 Antibody (ABIN281621) Images back to top
Antigen
Alternative Name IL-4 (IL4 Antibody Abstract)
Research Area Cytokines, Immunology, Virology, Inflammation, Cancer
Pathways JAK-STAT Signaling

Application Details

Product Details anti-IL-4 Antibody Target Details IL-4 Handling References for anti-IL-4 Antibody (ABIN281621) Images back to top
Application Notes Applications reported: The 8D4-8 antibody has been reported for use in capture of human IL-4 by ELISA and in intracellular staining for flow cytometric analysis.

Applications tested: The 8D4-8 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of human Interleukin-4 (IL-4) in combination with the biotinylated MP4-25D2 (13-7048) antibody for detection and recombinant human IL-4 (14-8049) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1-4 μg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 500 pg/mL - 4 pg/mL should be included in each ELISA plate. The fluorochrome-conjugated 8D4-8 antibody is offered in 2 formats: - μg size: has been tested by intracellular staining for flow cytometric analysis. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Restrictions For Research Use only

Handling

Product Details anti-IL-4 Antibody Target Details IL-4 Application Details References for anti-IL-4 Antibody (ABIN281621) Images back to top
Concentration 0.5 mg/mL
Buffer aqueous buffer, 0.09 % sodium azide, may contain carrier protein/stabilizer
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Monoclonal antibodies should not be stored at a temperature below -25 °C due to the aggregation effect of the protein.
Storage 4 °C

References for anti-IL-4 Antibody (ABIN281621)

Product Details anti-IL-4 Antibody Target Details IL-4 Application Details Handling Images back to top
Background publications

Bird, Wadhwa, Thorpe: "Development of immunoassays for human interleukin 3 and interleukin 4, some of which discriminate between different recombinant DNA-derived molecules." in: Cytokine, Vol. 3, Issue 6, pp. 562-7, 1992

Images

Product Details anti-IL-4 Antibody Target Details IL-4 Application Details Handling References for anti-IL-4 Antibody (ABIN281621) back to top
Supplier Images
 image for anti-IL-4 antibody (Interleukin 4) (ABIN281621) Human peripheral blood mononuclear cells were stimulated with PMA and ionomycin in th...