We are preparing the requested document. Please wait, this may take a while...!
|Application / Reactivity||Human|
|Biochemical Assay (BCA)||2 Antibodies|
|Blocking Antibody (Inhibition)||7 Antibodies|
|Dot Blot (DB)||5 Antibodies|
|ELISA (Capture)||3 Antibodies|
|ELISA (Detection)||4 Antibodies|
|ELISpot (Capture) (ELISPOT (Capture))||2 Antibodies|
|ELISpot (Detection) (ELISPOT (Detection))||2 Antibodies|
|Enzyme Immunoassay (EIA)||8 Antibodies|
|Flow Cytometry (FACS)||90 Antibodies|
|Functional Studies (Func)||11 Antibodies|
|Immunoassay (IA)||2 Antibodies|
|Immunocytochemistry (ICC)||4 Antibodies|
|Immunofluorescence (IF)||9 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||1 Antibodies|
|Immunohistochemistry (IHC)||61 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||10 Antibodies|
|Antigen||Interleukin 4 (IL4) Antibodies|
|Conjugate||This IL-4 antibody is conjugated to PE/Dazzle™ 594 Alternatives|
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC)
|10 references available|
|Supplier||Log in to see|
Product Details anti-IL-4 AntibodyTarget Details IL-4 Application Details Handling References for anti-IL-4 Antibody (ABIN2659777) Images
|Cross-Reactivity||Pig (Porcine), Rhesus Monkey|
|Purification||The antibody was purified by affinity chromatography and conjugated with PE/Dazzle™ 594 under optimal conditions. The solution is free of unconjugated PE/Dazzle™ 594 and unconjugated antibody.|
Target Details IL-4Product Details anti-IL-4 Antibody Application Details Handling References for anti-IL-4 Antibody (ABIN2659777) Images back to top
|Alternative Name||IL-4 (IL4 Antibody Abstract)|
|Background||IL-4 is a pleiotropic cytokine that is produced by activated T cells, mast cells, and basophils. IL-4 elicits many different biological responses but has two dominant functions. The first is regulating differentiation of naive CD4+ T cell to the Th2 type. Th2 cells produce IL-4, IL-5, IL-10, and IL-13, which tend to favor a humoral immune response while suppressing a cell-mediated immune response controlled by Th1 cells. The second is regulating IgE and IgG1 production by B cells.|
|Research Area||Cytokines, Immunology, Virology, Inflammation, Cancer|
Application DetailsProduct Details anti-IL-4 Antibody Target Details IL-4 Handling References for anti-IL-4 Antibody (ABIN2659777) Images back to top
|Application Notes||Optimal working dilution should be determined by the investigator.|
|Restrictions||For Research Use only|
HandlingProduct Details anti-IL-4 Antibody Target Details IL-4 Application Details References for anti-IL-4 Antibody (ABIN2659777) Images back to top
|Buffer||Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide and 0.2 % (w/v) BSA .|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Protect from prolonged exposure to light. Do not freeze.|
|Storage Comment||The antibody solution should be stored undiluted between 2°C and 8°C.|
References for anti-IL-4 Antibody (ABIN2659777)Product Details anti-IL-4 Antibody Target Details IL-4 Application Details Handling Images back to top
Iwamoto, Iwai, Tsujiyama et al.: "TNF-alpha drives human CD14+ monocytes to differentiate into CD70+ dendritic cells evoking Th1 and Th17 responses." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 179, Issue 3, pp. 1449-57, 2007 (PubMed).
Prussin, Metcalfe: "Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies." in: Journal of immunological methods, Vol. 188, Issue 1, pp. 117-28, 1996 (PubMed).
Andersson, Abrams, Björk et al.: "Concomitant in vivo production of 19 different cytokines in human tonsils." in: Immunology, Vol. 83, Issue 1, pp. 16-24, 1995 (PubMed).
Ramanathan, Ingram, Sullivan et al.: "Immunochemical mapping of domains in human interleukin 4 recognized by neutralizing monoclonal antibodies." in: Biochemistry, Vol. 32, Issue 14, pp. 3549-56, 1993 (PubMed).
Chrétien, Van Kimmenade, Pearce et al.: "Development of polyclonal and monoclonal antibodies for immunoassay and neutralization of human interleukin-4." in: Journal of immunological methods, Vol. 117, Issue 1, pp. 67-81, 1989 (PubMed).
Paul: "Interleukin-4: a prototypic immunoregulatory lymphokine." in: Blood, Vol. 77, Issue 9, pp. 1859-70, 1991 (PubMed).
Dullens, De Wit: "Cancer treatment with interleukins 1, 4 and 6 and combinations of cytokines: a review." in: In vivo (Athens, Greece), Vol. 5, Issue 6, pp. 567-70, 1992 (PubMed).
Boulay, Paul: "The interleukin-4 family of lymphokines." in: Current opinion in immunology, Vol. 4, Issue 3, pp. 294-8, 1992 (PubMed).
Abrams, Roncarolo, Yssel et al.: "Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples." in: Immunological reviews, Vol. 127, Issue 9-10, pp. 5-24, 1992 (PubMed).
Mahanty, Abrams, King et al.: "Parallel regulation of IL-4 and IL-5 in human helminth infections." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 148, Issue 11, pp. 3567-71, 1992 (PubMed).
ImagesProduct Details anti-IL-4 Antibody Target Details IL-4 Application Details Handling References for anti-IL-4 Antibody (ABIN2659777) back to top