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anti-Human IL-4 antibody for Immunofluorescence

Recommended IL-4 Antibody (supplied by: Log in to see )

Antigen
Interleukin 4 (IL4) Antibodies
  • BCGF-1
  • BCGF1
  • BSF-1
  • BSF1
  • IL-4
  • Il-4
  • IL2
  • IL4
  • Il4e12
Reactivity
Human
373
183
50
25
19
15
7
7
7
6
6
5
4
3
3
3
2
2
1
Host
Rat
247
199
195
8
7
2
Clonality
Monoclonal
Conjugate
This IL-4 antibody is conjugated to PE/Dazzle™ 594
93
50
34
23
22
8
8
7
6
4
4
4
3
2
2
2
2
2
2
2
2
2
2
1
1
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC)
347
344
192
83
56
37
36
31
28
27
24
23
20
20
10
6
6
5
3
3
3
2
2
2
2
1
1
1
1
1
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Catalog No. ABIN2659777
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00108149 ABIN2665169 IF IHC WB Rat IgG1 kappa Log in to see MP4-25D2 10
0.00108149 ABIN2659778 FACS IF IHC PE/Dazzle™ 594 Rat IgG1 kappa Log in to see MP4-25D2 11
0.00108149 ABIN218108 FACS IF ELISA WB Biotin Rat IgG1, kappa Log in to see
0.00108149 ABIN957657 FACS IF WB Rat IgG1, kappa Log in to see MP4-25D2
0.00108149 ABIN218107 FACS IF ELISA WB Rat IgG1, kappa Log in to see
0.00108149 ABIN2289123 FACS IF ELISA WB Biotin Rat IgG1 kappa Log in to see 5K302
0.00108149 ABIN4225956 ICC IF WB Alexa Fluor 647 Mouse IgG1 Log in to see 4D9
0.00108149 ABIN4225957 ICC IF WB Alexa Fluor 700 Mouse IgG1 Log in to see 4D9
0.00108149 ABIN4225958 ICC IF WB Allophycocyanin Mouse IgG1 Log in to see 4D9
0.00108149 ABIN4225959 ICC IF WB Biotin Mouse IgG1 Log in to see 4D9
0.00108149 ABIN4225960 ICC IF WB DyLight 650 Mouse IgG1 Log in to see 4D9
0.00108149 ABIN4225961 ICC IF WB FITC Mouse IgG1 Log in to see 4D9
0.00108149 ABIN4225962 ICC IF WB DyLight 680 Mouse IgG1 Log in to see 4D9
0.00108149 ABIN4225963 ICC IF WB DyLight 488 Mouse IgG1 Log in to see 4D9
0.00108149 ABIN4225965 ICC IF WB DyLight 755 Mouse IgG1 Log in to see 4D9
0.00108149 ABIN4225966 ICC IF WB PerCP Mouse IgG1 Log in to see 4D9
0.00108149 ABIN4225967 ICC IF WB PE Mouse IgG1 Log in to see 4D9
0.00108149 ABIN4225968 ICC IF WB DyLight 550 Mouse IgG1 Log in to see 4D9
0.00108149 ABIN4225969 ICC IF WB DyLight 350 Mouse IgG1 Log in to see 4D9
0.00108149 ABIN4225970 ICC IF WB DyLight 405 Mouse IgG1 Log in to see 4D9

Top referenced anti-IL-4 antibody for Immunofluorescence

Similar anti-IL-4 Antibodies

Application / Reactivity Human
Biochemical Assay (BCA) 2 Antibodies
Blocking Antibody (Inhibition) 7 Antibodies
Dot Blot (DB) 5 Antibodies
ELISA 181 Antibodies
ELISA (Capture) 3 Antibodies
ELISA (Detection) 4 Antibodies
ELISpot 7 Antibodies
ELISpot (Capture) (ELISPOT (Capture)) 2 Antibodies
ELISpot (Detection) (ELISPOT (Detection)) 2 Antibodies
Enzyme Immunoassay (EIA) 8 Antibodies
Flow Cytometry (FACS) 95 Antibodies
Functional Studies (Func) 11 Antibodies
Immunoassay (IA) 2 Antibodies
Immunocytochemistry (ICC) 22 Antibodies
Immunofluorescence (IF) 27 Antibodies
Immunohistochemistry (IHC) 61 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 10 Antibodies

General

Antigen Interleukin 4 (IL4) Antibodies
Reactivity Human
(373), (183), (50), (25), (19), (15), (7), (7), (7), (6), (6), (5), (4), (3), (3), (3), (2), (2), (1)
Host Rat
(247), (199), (195), (8), (7), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This IL-4 antibody is conjugated to PE/Dazzle™ 594
(93), (50), (34), (23), (22), (8), (8), (7), (6), (4), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC)
(347), (344), (192), (83), (56), (37), (36), (31), (28), (27), (24), (23), (20), (20), (10), (6), (6), (5), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 10 references available
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Product Details anti-IL-4 Antibody

Target Details IL-4 Application Details Handling References for anti-IL-4 Antibody (ABIN2659777) Images
Cross-Reactivity Pig (Porcine), Rhesus Monkey
Purification The antibody was purified by affinity chromatography and conjugated with PE/Dazzle™ 594 under optimal conditions. The solution is free of unconjugated PE/Dazzle™ 594 and unconjugated antibody.
Clone MP4-25D2
Isotype IgG1 kappa

Target Details IL-4

Product Details anti-IL-4 Antibody Application Details Handling References for anti-IL-4 Antibody (ABIN2659777) Images back to top
Antigen
Alternative Name IL-4 (IL4 Antibody Abstract)
Background IL-4 is a pleiotropic cytokine that is produced by activated T cells, mast cells, and basophils. IL-4 elicits many different biological responses but has two dominant functions. The first is regulating differentiation of naive CD4+ T cell to the Th2 type. Th2 cells produce IL-4, IL-5, IL-10, and IL-13, which tend to favor a humoral immune response while suppressing a cell-mediated immune response controlled by Th1 cells. The second is regulating IgE and IgG1 production by B cells.
Research Area Cytokines, Immunology, Virology, Inflammation, Cancer
Pathways JAK-STAT Signaling

Application Details

Product Details anti-IL-4 Antibody Target Details IL-4 Handling References for anti-IL-4 Antibody (ABIN2659777) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-IL-4 Antibody Target Details IL-4 Application Details References for anti-IL-4 Antibody (ABIN2659777) Images back to top
Buffer Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide and 0.2 % (w/v) BSA .
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Protect from prolonged exposure to light. Do not freeze.
Storage 4 °C
Storage Comment The antibody solution should be stored undiluted between 2°C and 8°C.

References for anti-IL-4 Antibody (ABIN2659777)

Product Details anti-IL-4 Antibody Target Details IL-4 Application Details Handling Images back to top
Background publications

Iwamoto, Iwai, Tsujiyama, Kurahashi, Takeshita, Naoe, Masunaga, Ogawa, Oguchi, Miyazaki: "TNF-alpha drives human CD14+ monocytes to differentiate into CD70+ dendritic cells evoking Th1 and Th17 responses." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 179, Issue 3, pp. 1449-57, 2007

Prussin, Metcalfe: "Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies." in: Journal of immunological methods, Vol. 188, Issue 1, pp. 117-28, 1996

Andersson, Abrams, Björk, Funa, Litton, Agren, Andersson: "Concomitant in vivo production of 19 different cytokines in human tonsils." in: Immunology, Vol. 83, Issue 1, pp. 16-24, 1995

Ramanathan, Ingram, Sullivan, Greenberg, Reim, Trotta, Le: "Immunochemical mapping of domains in human interleukin 4 recognized by neutralizing monoclonal antibodies." in: Biochemistry, Vol. 32, Issue 14, pp. 3549-56, 1993

Boulay, Paul: "The interleukin-4 family of lymphokines." in: Current opinion in immunology, Vol. 4, Issue 3, pp. 294-8, 1992

Dullens, De Wit: "Cancer treatment with interleukins 1, 4 and 6 and combinations of cytokines: a review." in: In vivo (Athens, Greece), Vol. 5, Issue 6, pp. 567-70, 1992

Abrams, Roncarolo, Yssel, Andersson, Gleich, Silver: "Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples." in: Immunological reviews, Vol. 127, Issue 9-10, pp. 5-24, 1992

Mahanty, Abrams, King, Limaye, Nutman: "Parallel regulation of IL-4 and IL-5 in human helminth infections." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 148, Issue 11, pp. 3567-71, 1992

Paul: "Interleukin-4: a prototypic immunoregulatory lymphokine." in: Blood, Vol. 77, Issue 9, pp. 1859-70, 1991

Chrétien, Van Kimmenade, Pearce, Banchereau, Abrams: "Development of polyclonal and monoclonal antibodies for immunoassay and neutralization of human interleukin-4." in: Journal of immunological methods, Vol. 117, Issue 1, pp. 67-81, 1989

Images

Product Details anti-IL-4 Antibody Target Details IL-4 Application Details Handling References for anti-IL-4 Antibody (ABIN2659777) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-IL-4 antibody (Interleukin 4)  (PE/Dazzle™ 594) (ABIN2659777) anti-Interleukin 4 (IL4) antibody (PE/Dazzle™ 594)
Flow Cytometry (FACS) image for anti-IL-4 antibody (Interleukin 4)  (PE/Dazzle™ 594) (ABIN2659777) anti-Interleukin 4 (IL4) antibody (PE/Dazzle™ 594) (Image 2)