anti-Human IL-4 antibody for Neutralization

Recommended IL-4 Antibody (supplied by: Log in to see )

Antigen
Interleukin 4 (IL4) Antibodies
  • IL4
  • IL-4
  • BCGF-1
  • BCGF1
  • BSF-1
  • BSF1
  • Il-4
  • Il4e12
  • IL2
  • interleukin 4
  • IL4
  • Il4
  • Il-4
Reactivity
Human
392
208
50
34
24
20
19
14
11
11
9
9
6
6
6
6
5
2
2
1
1
Host
Rat
266
218
203
7
6
4
Clonality
Monoclonal
Conjugate
This IL-4 antibody is un-conjugated
85
52
32
22
21
12
12
8
8
8
8
8
6
6
6
6
6
4
4
4
4
4
3
2
2
2
2
1
1
Application
Intracellular Staining (ICS), Neutralization (Neut), ELISA
386
374
255
126
55
46
43
39
39
23
22
22
15
10
9
7
4
3
3
3
3
3
3
2
2
2
1
1
1
1
1
1
1
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Catalog No. ABIN1177267
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN964756 IHC Neut RIA ELISA WB Rabbit IgG Log in to see Polyclonal
1 ABIN1102500 IHC (p) Neut ELISA WB Rabbit IgG Log in to see Polyclonal
1 ABIN2451297 IP IHC (p) Neut ELISA WB Rabbit IgG AA 25-153 Log in to see Polyclonal
1 ABIN1889568 Neut IHC WB Mouse IgG1 AA 25-153 Log in to see MM0404-4Z33
1 ABIN4325241 BP IHC IHC (fro) Neut WB Mouse IgG1 Log in to see MM0404-4Z33
1 ABIN962202 Neut Rat IgG1, kappa Log in to see MP4-25D2
1 ABIN2290325 IP Neut IHC ELISA WB Rabbit IgG Log in to see Polyclonal
1 ABIN2568260 Func Neut Rat IgG1 kappa Log in to see MP4-25D2
1 ABIN2568261 Func Neut Rat IgG1 kappa Log in to see MP4-25D2
1 ABIN2290130 Neut Rabbit IgG Log in to see 13G43
1 ABIN4325267 BP ICC IF Neut WB Mouse IgG1 Log in to see 4D9
1 ABIN346565 Neut ELISA WB Rabbit Log in to see Polyclonal
1 ABIN3031481 IP Neut IHC (p) ELISA WB Rabbit IgG AA 25-153 Log in to see Polyclonal
1 ABIN2289255 FACS IF Neut ELISA WB Rat IgG1 kappa Log in to see 5K302
1 ABIN2289216 Neut IHC ELISA WB Alkaline Phosphatase (AP) Rabbit IgG Log in to see Polyclonal
1 ABIN2289109 Neut IHC ELISA WB APC Rabbit IgG Log in to see Polyclonal
1 ABIN2289112 Neut IHC ELISA WB Biotin Rabbit IgG Log in to see Polyclonal
1 ABIN2289113 Neut IHC ELISA WB FITC Rabbit IgG Log in to see Polyclonal
1 ABIN2289117 Neut IHC ELISA WB PE Rabbit IgG Log in to see Polyclonal
1 ABIN2289115 Neut IHC ELISA WB HRP Rabbit IgG Log in to see Polyclonal

General

Antigen Interleukin 4 (IL4) Antibodies
Reactivity Human
(392), (208), (50), (34), (24), (20), (19), (14), (11), (11), (9), (9), (6), (6), (6), (6), (5), (2), (2), (1), (1)
Host Rat
(266), (218), (203), (7), (6), (4)
Clonality (Clone)
Monoclonal   ( )
Conjugate This IL-4 antibody is un-conjugated
(85), (52), (32), (22), (21), (12), (12), (8), (8), (8), (8), (8), (6), (6), (6), (6), (6), (4), (4), (4), (4), (4), (3), (2), (2), (2), (2), (1), (1)
Application Intracellular Staining (ICS), Neutralization (Neut), ELISA
(386), (374), (255), (126), (55), (46), (43), (39), (39), (23), (22), (22), (15), (10), (9), (7), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-IL-4 Antibody

Target Details IL-4 Application Details Handling ProductDetails: References for anti-IL-4 antibody (ABIN1177267) Images
Brand BD Pharmingen™
Characteristics Neutralization Activity: This antibody has been reported to be useful for the neutralization of recombinant human IL-4. Neutralization may be measured with a proliferation assay using 2 ng/mL recombinant human IL-4 (Cat. No. 554605) to stimulate TF-1 cells at 1x10^5 cells/mL as indicator cells (i.e. preincubation of the antibody with recombinant human IL-4 for 60 minutes).
50% Neutralization (ND50) at 4 - 20 ng/mL
>95% Neutralization at 20 - 100 ng/mL
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Sterility 0.2 μm filtered
Endotoxin Level Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.
Immunogen Purified Recombinant Human IL-4
Clone MP4-25D2
Isotype IgG1

Target Details IL-4

Product Details anti-IL-4 Antibody Application Details Handling ProductDetails: References for anti-IL-4 antibody (ABIN1177267) Images back to top
Antigen
Alternative Name IL-4 (IL4 Antibody Abstract)
Background The MP4-25D2 antibody reacts with human interleukin-4 (IL-4). The immunogen used to generate the MP4-25D2 hybridoma was purified recombinant human IL-4. This is a neutralizing antibody. The MP4-25D2 antibody has been reported to cross react with IL-4 from rhesus monkeys. The use of the MP4-25D2 antibody for epitope mapping of human IL-4 has been described.
Research Area Cancer
Pathways JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation

Application Details

Product Details anti-IL-4 Antibody Target Details IL-4 Handling ProductDetails: References for anti-IL-4 antibody (ABIN1177267) Images back to top
Application Notes ELISA: Biotinylated MP4-25D2 antibody (Cat. No. 554483) has been reported to be useful as a detection antibody for sandwich ELISA measuring human IL-4 protein levels. Biotinylated MP4-25D2 antibody can be paired with the purified 8D4-8 antibody (Cat. No. 554515) as the capture antibody, with recombinant human IL-4 (Cat. No. 554605) as the standard. Investigators are encouraged to use the BD OptEIA™ Human IL-4 ELISA (Cat. No. 555194 or 550614) for measuring human IL-4 in serum or plasma samples.
Restrictions For Research Use only

Handling

Product Details anti-IL-4 Antibody Target Details IL-4 Application Details ProductDetails: References for anti-IL-4 antibody (ABIN1177267) Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.
Preservative Azide free
Storage 4 °C
Storage Comment Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

ProductDetails: References for anti-IL-4 antibody (ABIN1177267)

Product Details anti-IL-4 Antibody Target Details IL-4 Application Details Handling Images back to top
Product cited in:

Prussin, Metcalfe: "Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies." in: Journal of immunological methods, Vol. 188, Issue 1, pp. 117-28, 1996

Ramanathan, Ingram, Sullivan, Greenberg, Reim, Trotta, Le: "Immunochemical mapping of domains in human interleukin 4 recognized by neutralizing monoclonal antibodies." in: Biochemistry, Vol. 32, Issue 14, pp. 3549-56, 1993

Chrétien, Van Kimmenade, Pearce, Banchereau, Abrams: "Development of polyclonal and monoclonal antibodies for immunoassay and neutralization of human interleukin-4." in: Journal of immunological methods, Vol. 117, Issue 1, pp. 67-81, 1989

Kitamura, Tange, Terasawa, Chiba, Kuwaki, Miyagawa, Piao, Miyazono, Urabe, Takaku: "Establishment and characterization of a unique human cell line that proliferates dependently on GM-CSF, IL-3, or erythropoietin." in: Journal of cellular physiology, Vol. 140, Issue 2, pp. 323-34, 1989