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|Application / Reactivity||Human|
|Biochemical Assay (BCA)||2 Antibodies|
|Blocking Antibody (Inhibition)||7 Antibodies|
|Dot Blot (DB)||5 Antibodies|
|ELISA (Capture)||3 Antibodies|
|ELISA (Detection)||4 Antibodies|
|ELISpot (Capture) (ELISPOT (Capture))||2 Antibodies|
|ELISpot (Detection) (ELISPOT (Detection))||2 Antibodies|
|Enzyme Immunoassay (EIA)||8 Antibodies|
|Flow Cytometry (FACS)||90 Antibodies|
|Functional Studies (Func)||11 Antibodies|
|Immunoassay (IA)||2 Antibodies|
|Immunocytochemistry (ICC)||4 Antibodies|
|Immunofluorescence (IF)||9 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||1 Antibodies|
|Immunohistochemistry (IHC)||61 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||10 Antibodies|
|Antigen||Interleukin 4 (IL4) Antibodies|
|Conjugate||This IL-4 antibody is un-conjugated Alternatives|
Intracellular Staining (ICS), Neutralization (Neut), ELISA
|4 references available|
|Supplier||Log in to see|
Product Details anti-IL-4 AntibodyTarget Details IL-4 Application Details Handling References for anti-IL-4 Antibody (ABIN1177267) Images
Neutralization Activity: This antibody has been reported to be useful for the neutralization of recombinant human IL-4. Neutralization may be measured with a proliferation assay using 2 ng/mL recombinant human IL-4 (Cat. No. 554605) to stimulate TF-1 cells at 1x10^5 cells/mL as indicator cells (i.e. preincubation of the antibody with recombinant human IL-4 for 60 minutes).
50% Neutralization (ND50) at 4 - 20 ng/mL
>95% Neutralization at 20 - 100 ng/mL
|Purification||The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.|
|Sterility||0.2 μm filtered|
|Endotoxin Level||Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.|
|Immunogen||Purified Recombinant Human IL-4|
Target Details IL-4Product Details anti-IL-4 Antibody Application Details Handling References for anti-IL-4 Antibody (ABIN1177267) Images back to top
|Alternative Name||IL-4 (IL4 Antibody Abstract)|
|Background||The MP4-25D2 antibody reacts with human interleukin-4 (IL-4). The immunogen used to generate the MP4-25D2 hybridoma was purified recombinant human IL-4. This is a neutralizing antibody. The MP4-25D2 antibody has been reported to cross react with IL-4 from rhesus monkeys. The use of the MP4-25D2 antibody for epitope mapping of human IL-4 has been described.|
Application DetailsProduct Details anti-IL-4 Antibody Target Details IL-4 Handling References for anti-IL-4 Antibody (ABIN1177267) Images back to top
|Application Notes||ELISA: Biotinylated MP4-25D2 antibody (Cat. No. 554483) has been reported to be useful as a detection antibody for sandwich ELISA measuring human IL-4 protein levels. Biotinylated MP4-25D2 antibody can be paired with the purified 8D4-8 antibody (Cat. No. 554515) as the capture antibody, with recombinant human IL-4 (Cat. No. 554605) as the standard. Investigators are encouraged to use the BD OptEIA™ Human IL-4 ELISA (Cat. No. 555194 or 550614) for measuring human IL-4 in serum or plasma samples.|
|Restrictions||For Research Use only|
HandlingProduct Details anti-IL-4 Antibody Target Details IL-4 Application Details References for anti-IL-4 Antibody (ABIN1177267) Images back to top
|Buffer||No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.|
|Storage Comment||Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.|
References for anti-IL-4 Antibody (ABIN1177267)Product Details anti-IL-4 Antibody Target Details IL-4 Application Details Handling Images back to top
|Product cited in:||
Prussin, Metcalfe: "Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies." in: Journal of immunological methods, Vol. 188, Issue 1, pp. 117-28, 1996 (PubMed).
Ramanathan, Ingram, Sullivan et al.: "Immunochemical mapping of domains in human interleukin 4 recognized by neutralizing monoclonal antibodies." in: Biochemistry, Vol. 32, Issue 14, pp. 3549-56, 1993 (PubMed).
Kitamura, Tange, Terasawa et al.: "Establishment and characterization of a unique human cell line that proliferates dependently on GM-CSF, IL-3, or erythropoietin." in: Journal of cellular physiology, Vol. 140, Issue 2, pp. 323-34, 1989 (PubMed).
Chrétien, Van Kimmenade, Pearce et al.: "Development of polyclonal and monoclonal antibodies for immunoassay and neutralization of human interleukin-4." in: Journal of immunological methods, Vol. 117, Issue 1, pp. 67-81, 1989 (PubMed).