anti-Human IL-4 antibody for Western Blotting

Recommended IL-4 Antibody (supplied by: Log in to see )

Antigen
Interleukin 4 (IL4) Antibodies
  • IL4
  • IL-4
  • BCGF-1
  • BCGF1
  • BSF-1
  • BSF1
  • Il-4
  • Il4e12
  • IL2
  • interleukin 4
  • IL4
  • Il4
  • Il-4
Epitope
Middle Region
25
21
13
11
9
9
7
6
6
5
4
4
4
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Human
390
208
50
34
24
20
19
14
11
11
9
9
6
6
6
6
5
2
2
1
1
Host
Rabbit
262
218
205
7
6
4
Clonality
Polyclonal
Conjugate
This IL-4 antibody is un-conjugated
86
52
32
22
21
12
12
8
8
8
8
8
6
6
6
6
6
4
4
4
4
4
3
2
2
2
2
1
1
Application
Western Blotting (WB)
388
370
255
126
56
46
42
39
39
23
22
22
15
10
9
8
4
3
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
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Catalog No. ABIN2785656
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.3484411 ABIN2785657 WB Rabbit Middle Region Log in to see Polyclonal 1
3.3484411 ABIN119373 EIA FACS IHC (fro) WB Rat IgG1 Log in to see MP4-25D2 6
3.3484411 ABIN651434 FACS IHC (p) WB Rabbit Ig AA 122-150, C-Term Log in to see Polyclonal 1
3.3484411 ABIN3042695 WB Rabbit IgG AA 72-98, Middle Region Log in to see Polyclonal 5
3.3484411 ABIN3044141 IHC (p) WB Rabbit IgG AA 72-88, Middle Region Log in to see Polyclonal 4
3.3484411 ABIN964769 ELISA WB HRP Rabbit IgG Log in to see Polyclonal
3.3484411 ABIN964778 ELISA WB Biotin Rabbit IgG Log in to see Polyclonal
3.3484411 ABIN3187690 ELISA IHC (p) WB Rabbit IgG Internal Region Log in to see Polyclonal
3.3484411 ABIN255816 FACS IHC (fro) IHC ELISA WB Rat IgG1 Log in to see MP4-25D2
3.3484411 ABIN2706390 WB Rabbit Center Log in to see Polyclonal
3.3484411 ABIN2706391 IP WB Rabbit Center Log in to see Polyclonal
3.3484411 ABIN2704922 WB Rabbit C-Term Log in to see Polyclonal
3.3484411 ABIN964756 IHC Neut RIA ELISA WB Rabbit IgG Log in to see Polyclonal
3.3484411 ABIN498521 WB Rabbit Log in to see Polyclonal
3.3484411 ABIN439189 IHC (p) WB Rabbit Log in to see Polyclonal
3.3484411 ABIN1859431 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 66-120 Log in to see Polyclonal
3.3484411 ABIN3031478 FACS IHC ELISA WB Rabbit Ig Fraction AA 122-150 Log in to see Polyclonal
3.3484411 ABIN634805 WB Rabbit Middle Region Log in to see Polyclonal
3.3484411 ABIN634804 WB Rabbit Middle Region Log in to see Polyclonal
3.3484411 ABIN517020 WB Mouse AA 1-153, full length Log in to see Polyclonal

Top referenced anti-IL-4 antibody for Western Blotting

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Application / Reactivity Human
Biochemical Assay (BCA) 2 Antibodies
Blocking Antibody (Inhibition) 6 Antibodies
Blocking Peptide (BP) 2 Antibodies
Cell Culture (CC) 1 Antibodies
Dot Blot (DB) 3 Antibodies
ELISA 190 Antibodies
ELISA (Capture) 2 Antibodies
ELISA (Detection) 3 Antibodies
ELISpot 7 Antibodies
ELISpot (Capture) (ELISPOT (Capture)) 1 Antibodies
ELISpot (Detection) (ELISPOT (Detection)) 1 Antibodies
Enzyme Immunoassay (EIA) 10 Antibodies
Flow Cytometry (FACS) 125 Antibodies
Functional Studies (Func) 11 Antibodies
Immunoassay (IA) 2 Antibodies
Immunocytochemistry (ICC) 26 Antibodies
Immunofluorescence (IF) 31 Antibodies

General

Antigen Interleukin 4 (IL4) Antibodies
Epitope Middle Region
(25), (21), (13), (11), (9), (9), (7), (6), (6), (5), (4), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(390), (208), (50), (34), (24), (20), (19), (14), (11), (11), (9), (9), (6), (6), (6), (6), (5), (2), (2), (1), (1)
Host Rabbit
(262), (218), (205), (7), (6), (4)
Clonality
Polyclonal
Conjugate This IL-4 antibody is un-conjugated
(86), (52), (32), (22), (21), (12), (12), (8), (8), (8), (8), (8), (6), (6), (6), (6), (6), (4), (4), (4), (4), (4), (3), (2), (2), (2), (2), (1), (1)
Application Western Blotting (WB)
(388), (370), (255), (126), (56), (46), (42), (39), (39), (23), (22), (22), (15), (10), (9), (8), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-IL-4 Antibody

Target Details IL-4 Application Details Handling ProductDetails: References for anti-IL-4 antibody (ABIN2785656) Images
Blocking Peptide Blocking peptide for this product available: ABIN978510
Sequence TVLRQFYSHH EKDTRCLGAT AQQFHRHKQL IRFLKRLDRN LWGLAGLNSC
Predicted Reactivity Human: 100%
Characteristics This is a rabbit polyclonal antibody against IL4. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the middle region of human IL4

Target Details IL-4

Product Details anti-IL-4 Antibody Application Details Handling ProductDetails: References for anti-IL-4 antibody (ABIN2785656) Images back to top
Antigen
Alternative Name IL4 (IL4 Antibody Abstract)
Background IL4 is a pleiotropic cytokine produced by activated T cells. This cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. The protein encoded by this gene is a pleiotropic cytokine produced by activated T cells. This cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. This gene, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.
Alias Symbols: BSF1, IL-4, MGC79402, BCGF1, BSF-1, BCGF-1
Protein Interaction Partner: IL13, IL4, PIK3CD, IL2RG, IL13RA2, CD53, IL4R, A2M,
Protein Size: 153
Molecular Weight 15 kDa
Gene ID 3565
NCBI Accession NM_000589, NP_000580
UniProt P05112
Research Area Immunology, Adaptive Immunity, Cytokines, Innate Immunity
Pathways JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation

Application Details

Product Details anti-IL-4 Antibody Target Details IL-4 Handling ProductDetails: References for anti-IL-4 antibody (ABIN2785656) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 153 AA

Restrictions For Research Use only

Handling

Product Details anti-IL-4 Antibody Target Details IL-4 Application Details ProductDetails: References for anti-IL-4 antibody (ABIN2785656) Images back to top
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

ProductDetails: References for anti-IL-4 antibody (ABIN2785656)

Product Details anti-IL-4 Antibody Target Details IL-4 Application Details Handling Images back to top
Background publications

Pachkoria, Lucena, Crespo, Ruiz-Cabello, Lopez-Ortega, Fernandez, Romero-Gomez, Madrazo, Durán, de Dios, Borraz, Navarro, Andrade: "Analysis of IL-10, IL-4 and TNF-alpha polymorphisms in drug-induced liver injury (DILI) and its outcome." in: Journal of hepatology, Vol. 49, Issue 1, pp. 107-14, 2008

Images

Product Details anti-IL-4 Antibody Target Details IL-4 Application Details Handling ProductDetails: References for anti-IL-4 antibody (ABIN2785656) back to top
Supplier Images
Western Blotting (WB) image for anti-IL-4 antibody (Interleukin 4) (Middle Region) (ABIN2785656) WB Suggested Anti-IL4 Antibody Titration: 0.2-1 ug/ml
Positive Control: ...