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anti-Human IL-4 antibody for Western Blotting

Recommended IL-4 Antibody (supplied by: Log in to see )

Antigen
Interleukin 4 (IL4) Antibodies
  • BCGF-1
  • BCGF1
  • BSF-1
  • BSF1
  • IL-4
  • Il-4
  • IL2
  • IL4
  • Il4e12
Epitope
Middle Region
23
14
13
12
11
9
7
6
5
4
4
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
Reactivity
Human
399
206
49
25
23
19
14
7
7
7
6
6
5
4
3
3
3
2
2
1
Host
Rabbit
265
215
215
8
7
2
Clonality
Polyclonal
Conjugate
This IL-4 antibody is un-conjugated
92
53
33
22
21
11
11
8
8
7
7
7
6
6
6
6
6
4
4
4
4
4
3
2
1
1
1
1
Application
Western Blotting (WB)
408
370
251
114
57
55
40
37
34
30
22
20
18
10
8
6
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
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Catalog No. ABIN2785657
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.12326975 ABIN2785656 WB Rabbit Middle Region Log in to see Polyclonal 1
0.12326975 ABIN119373 EIA FACS IHC (fro) WB Rat IgG1 Log in to see MP4-25D2 6
0.12326975 ABIN651434 FACS IHC (p) WB Rabbit Ig AA 122-150, C-Term Log in to see Polyclonal 1
0.12326975 ABIN964769 ELISA WB HRP Rabbit IgG Log in to see Polyclonal
0.12326975 ABIN964778 ELISA WB Biotin Rabbit IgG Log in to see Polyclonal
0.12326975 ABIN3044141 IHC (p) WB Rabbit IgG AA 72-88, Middle Region Log in to see Polyclonal
0.12326975 ABIN3042695 WB Rabbit IgG AA 72-98, Middle Region Log in to see Polyclonal
0.12326975 ABIN255816 FACS IHC (fro) IHC ELISA WB Rat IgG1 Log in to see MP4-25D2
0.12326975 ABIN964756 IHC Neut RIA ELISA WB Rabbit IgG Log in to see Polyclonal
0.12326975 ABIN2706390 WB Rabbit Center Log in to see Polyclonal
0.12326975 ABIN2706391 IP WB Rabbit Center Log in to see Polyclonal
0.12326975 ABIN2704922 WB Rabbit C-Term Log in to see Polyclonal
0.12326975 ABIN498521 WB Rabbit Log in to see Polyclonal
0.12326975 ABIN439189 IHC (p) WB Rabbit Log in to see Polyclonal
0.12326975 ABIN2488029 WB Rat IgG1 Log in to see
0.12326975 ABIN3187690 ELISA IHC (p) WB Rabbit IgG Internal Region Log in to see Polyclonal
0.12326975 ABIN517020 WB Mouse AA 1-153, full length Log in to see Polyclonal
0.12326975 ABIN1859431 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 66-120 Log in to see Polyclonal
0.12326975 ABIN634804 WB Rabbit Middle Region Log in to see Polyclonal
0.12326975 ABIN634805 WB Rabbit Middle Region Log in to see Polyclonal

Top referenced anti-IL-4 antibody for Western Blotting

Similar anti-IL-4 Antibodies

Application / Reactivity Human
Biochemical Assay (BCA) 2 Antibodies
Blocking Antibody (Inhibition) 7 Antibodies
Blocking Peptide (BP) 2 Antibodies
Dot Blot (DB) 5 Antibodies
ELISA 208 Antibodies
ELISA (Capture) 2 Antibodies
ELISA (Detection) 2 Antibodies
ELISpot 7 Antibodies
ELISpot (Capture) (ELISPOT (Capture)) 1 Antibodies
ELISpot (Detection) (ELISPOT (Detection)) 1 Antibodies
Enzyme Immunoassay (EIA) 8 Antibodies
Flow Cytometry (FACS) 121 Antibodies
Functional Studies (Func) 11 Antibodies
Immunoassay (IA) 2 Antibodies
Immunocytochemistry (ICC) 23 Antibodies
Immunofluorescence (IF) 29 Antibodies
Immunohistochemistry (IHC) 75 Antibodies

General

Antigen Interleukin 4 (IL4) Antibodies
Epitope Middle Region
(23), (14), (13), (12), (11), (9), (7), (6), (5), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity Human
(399), (206), (49), (25), (23), (19), (14), (7), (7), (7), (6), (6), (5), (4), (3), (3), (3), (2), (2), (1)
Host Rabbit
(265), (215), (215), (8), (7), (2)
Clonality
Polyclonal
Conjugate This IL-4 antibody is un-conjugated
(92), (53), (33), (22), (21), (11), (11), (8), (8), (7), (7), (7), (6), (6), (6), (6), (6), (4), (4), (4), (4), (4), (3), (2), (1), (1), (1), (1)
Application Western Blotting (WB)
(408), (370), (251), (114), (57), (55), (40), (37), (34), (30), (22), (20), (18), (10), (8), (6), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-IL-4 Antibody

Target Details IL-4 Application Details Handling References for anti-IL-4 Antibody (ABIN2785657) Images
Blocking Peptide Blocking peptide for this product available: ABIN978511
Sequence LIRFLKRLDR NLWGLAGLNS CPVKEANQST LENFLERLKT IMREKYSKCS
Predicted Reactivity Human : 100 %
Characteristics This is a rabbit polyclonal antibody against IL4. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the middle region of human IL4

Target Details IL-4

Product Details anti-IL-4 Antibody Application Details Handling References for anti-IL-4 Antibody (ABIN2785657) Images back to top
Antigen
Alternative Name IL4 (IL4 Antibody Abstract)
Background IL4 participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes.The protein encoded by this gene is a pleiotropic cytokine produced by activated T cells. This cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. This gene, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.
Molecular Weight 15 kDa
Gene ID 3565
NCBI Accession NM_000589, NP_000580
UniProt P05112
Research Area Immunology, Adaptive Immunity, Cytokines, Innate Immunity
Pathways JAK-STAT Signaling

Application Details

Product Details anti-IL-4 Antibody Target Details IL-4 Handling References for anti-IL-4 Antibody (ABIN2785657) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 153 AA

Restrictions For Research Use only

Handling

Product Details anti-IL-4 Antibody Target Details IL-4 Application Details References for anti-IL-4 Antibody (ABIN2785657) Images back to top
Format Liquid
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C,-20 °C
Storage Comment For short term use, store at 2-8 °C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-IL-4 Antibody (ABIN2785657)

Product Details anti-IL-4 Antibody Target Details IL-4 Application Details Handling Images back to top
Background publications

Pachkoria, Lucena, Crespo, Ruiz-Cabello, Lopez-Ortega, Fernandez, Romero-Gomez, Madrazo, Durán, de Dios, Borraz, Navarro, Andrade: "Analysis of IL-10, IL-4 and TNF-alpha polymorphisms in drug-induced liver injury (DILI) and its outcome." in: Journal of hepatology, Vol. 49, Issue 1, pp. 107-14, 2008

Images

Product Details anti-IL-4 Antibody Target Details IL-4 Application Details Handling References for anti-IL-4 Antibody (ABIN2785657) back to top
Supplier Images
Western Blotting (WB) image for anti-IL-4 antibody (Interleukin 4) (Middle Region) (ABIN2785657) WB Suggested Anti-IL4 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1...