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anti-Mouse (Murine) IL-4 antibody for Functional Studies

Recommended IL-4 Antibody (supplied by: Log in to see )

Antigen
Interleukin 4 (IL4) Antibodies
  • BCGF-1
  • BCGF1
  • BSF-1
  • BSF1
  • IL-4
  • Il-4
  • IL2
  • IL4
  • Il4e12
Reactivity
Mouse (Murine)
407
214
50
25
22
19
14
7
7
7
6
6
5
4
3
3
3
2
2
1
Host
Rabbit
267
226
220
8
7
2
Clonality
Polyclonal
Conjugate
This IL-4 antibody is un-conjugated
95
53
35
23
23
13
11
8
8
7
7
7
7
6
6
6
6
6
6
4
4
4
4
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Functional Studies (Func), Western Blotting (WB)
410
373
260
116
58
55
39
37
34
30
23
23
20
19
10
6
6
5
3
3
3
2
2
2
2
2
1
1
1
1
1
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Catalog No. ABIN115882
$ 368.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00087626296 ABIN115881 EIA Func WB Rabbit Log in to see Polyclonal 2
0.00087626296 ABIN264018 Func WB Rat IgG1 Log in to see I4-F5
0.00087626296 ABIN282507 ELISA (Capture) ELISpot Func Rat IgG1 kappa Log in to see 11B11
0.00087626296 ABIN208728 Func Neut ELISA WB Rabbit IgG Log in to see Polyclonal
0.00087626296 ABIN2475015 Func ELISA WB Rabbit IgG Log in to see Polyclonal 1
0.00087626296 ABIN2568255 Func FACS IF IHC ELISA Rat IgG1 kappa Log in to see 11B11
0.00087626296 ABIN2568254 Func FACS IF IHC ELISA Rat IgG1 kappa Log in to see 11B11
0.00087626296 ABIN2144133 Func FACS Mouse IgG1 kappa Log in to see 8D4-8
0.00087626296 ABIN641027 Func ELISA WB Rabbit Log in to see

Top referenced anti-IL-4 antibody for Functional Studies

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Application / Reactivity Mouse (Murine)
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Blocking Antibody (Inhibition) 2 Antibodies
Cellular Assay (CA) 1 Antibodies
Dot Blot (DB) 1 Antibodies
ELISA 129 Antibodies
ELISA (Capture) 3 Antibodies
ELISA (Detection) 1 Antibodies
ELISpot 8 Antibodies
ELISpot (Capture) (ELISPOT (Capture)) 1 Antibodies
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Flow Cytometry (FACS) 109 Antibodies
Functional Studies (Func) 10 Antibodies
Immunoassay (IA) 1 Antibodies
Immunocytochemistry (ICC) 6 Antibodies
Immunofluorescence (IF) 10 Antibodies
Immunohistochemistry (IHC) 37 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 2 Antibodies

General

Antigen Interleukin 4 (IL4) Antibodies
Reactivity Mouse (Murine)
(407), (214), (50), (25), (22), (19), (14), (7), (7), (7), (6), (6), (5), (4), (3), (3), (3), (2), (2), (1)
Host Rabbit
(267), (226), (220), (8), (7), (2)
Clonality
Polyclonal
Conjugate This IL-4 antibody is un-conjugated
(95), (53), (35), (23), (23), (13), (11), (8), (8), (7), (7), (7), (7), (6), (6), (6), (6), (6), (6), (4), (4), (4), (4), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Functional Studies (Func), Western Blotting (WB)
(410), (373), (260), (116), (58), (55), (39), (37), (34), (30), (23), (23), (20), (19), (10), (6), (6), (5), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-IL-4 Antibody

Target Details IL-4 Application Details Handling References for anti-IL-4 Antibody (ABIN115882) Images
Specificity Recognises Mouse Interleukin-4 (IL-4).
Purification Affinity chromatography.
Immunogen Highly pure (>98%) recombinant mIL-4 (murine Interleukin-4).

Target Details IL-4

Product Details anti-IL-4 Antibody Application Details Handling References for anti-IL-4 Antibody (ABIN115882) Images back to top
Antigen
Alternative Name Interleukin-4 / IL4 (IL4 Antibody Abstract)
Background IL4 is a pleiotropic cytokine produced by activated T cells, mast cells and basophils. It is a ligand for Interleukin 4 receptor. The Interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. IL4 elicits many different biological responses, but has two dominant functions. The first is regulating differentiation of naive CD4+ T cell to the Th2 type. Th2 cells produce IL4, IL5, IL10 and IL13, which tend to favor a humoral immune response while suppressing a cell mediated immune response controlled by Th1 cells. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. The second is regulating IgE and IgG1 production by B cells. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.Synonyms: B-cell stimulatory factor 1, BSF1, Binetrakin, IL-4, Lymphocyte stimulatory factor 1, Pitrakinra
Gene ID 16189
NCBI Accession NP_067258
UniProt P07750
Pathways JAK-STAT Signaling

Application Details

Product Details anti-IL-4 Antibody Target Details IL-4 Handling References for anti-IL-4 Antibody (ABIN115882) Images back to top
Application Notes Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of mIL-4 (7.5 ng/mL), aconcentration of 0.25-0.40 μg/mL of this antibody is required. ELISA: (Indirect): To detect mIL-4 by indirect ELISA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with compatible secondary reagents, allows the detection of atleast 0.2-0.4 ng/well of recombinant mIL-4. (Sandwich): To detect mIL-4 by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with Biotinylated Anti-Murine IL-4 (PP011B) as a detectionantibody, allows the detection of at least 0.2-0.4 ng/well of recombinant mIL-4. Western Blot: To detect mIL-4 by Western Blot analysis this antibody can be used at a concentration of
Restrictions For Research Use only

Handling

Product Details anti-IL-4 Antibody Target Details IL-4 Application Details References for anti-IL-4 Antibody (ABIN115882) Images back to top
Reconstitution Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
Concentration 1.0 mg/mL
Buffer PBS, pH 7.2 without preservatives.
Preservative Without preservative
Handling Advice Avoid repeated freezing and thawing. Centrifuge vial prior to opening!
Storage 4 °C/-20 °C
Storage Comment Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

References for anti-IL-4 Antibody (ABIN115882)

Product Details anti-IL-4 Antibody Target Details IL-4 Application Details Handling Images back to top
Background publications

Gutermuth, Bewersdorff, Traidl-Hoffmann, Ring, Mueller, Behrendt, Jakob: "Immunomodulatory effects of aqueous birch pollen extracts and phytoprostanes on primary immune responses in vivo." in: The Journal of allergy and clinical immunology, Vol. 120, Issue 2, pp. 293-9, 2007

Tu, Fang, Artis, Shestova, Pross, Maillard, Pear: "Notch signaling is an important regulator of type 2 immunity." in: The Journal of experimental medicine, Vol. 202, Issue 8, pp. 1037-42, 2005