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anti-Rat (Rattus) IL-4 antibody for Enzyme Immunoassay

Recommended IL-4 Antibody (supplied by: Log in to see )

Antigen
Interleukin 4 (IL4) Antibodies
  • BCGF-1
  • BCGF1
  • BSF-1
  • BSF1
  • IL-4
  • Il-4
  • IL2
  • IL4
  • Il4e12
Reactivity
Rat (Rattus)
410
218
50
25
22
19
15
7
7
7
6
6
5
4
3
3
3
2
2
1
Host
Rabbit
268
230
220
8
7
2
Clonality
Polyclonal
Conjugate
This IL-4 antibody is conjugated to Biotin
94
53
35
23
23
13
11
8
8
7
7
7
7
6
6
6
6
6
6
4
4
4
4
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
414
376
262
118
58
57
39
37
34
31
24
23
20
19
10
6
6
5
3
3
3
2
2
2
2
2
1
1
1
1
1
Supplier
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Catalog No. ABIN115955
$ 286.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008867729 ABIN115956 EIA WB Biotin Rabbit Log in to see Polyclonal
0.0008867729 ABIN115957 EIA Func WB Rabbit Log in to see Polyclonal
0.0008867729 ABIN115958 EIA Func WB Rabbit Log in to see Polyclonal

Similar anti-IL-4 Antibodies

Application / Reactivity Rat (Rattus)
Blocking Antibody (Inhibition) 1 Antibodies
Blocking Reagent (BR) 1 Antibodies
ELISA 36 Antibodies
ELISpot 4 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies
Flow Cytometry (FACS) 4 Antibodies
Functional Studies (Func) 3 Antibodies
Immunocytochemistry (ICC) 2 Antibodies
Immunohistochemistry (IHC) 2 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 3 Antibodies
Intracellular Staining (ICS) 2 Antibodies
Neutralization (Neut) 3 Antibodies
Western Blotting (WB) 36 Antibodies

General

Antigen Interleukin 4 (IL4) Antibodies
Reactivity Rat (Rattus)
(410), (218), (50), (25), (22), (19), (15), (7), (7), (7), (6), (6), (5), (4), (3), (3), (3), (2), (2), (1)
Host Rabbit
(268), (230), (220), (8), (7), (2)
Clonality
Polyclonal
Conjugate This IL-4 antibody is conjugated to Biotin
(94), (53), (35), (23), (23), (13), (11), (8), (8), (7), (7), (7), (7), (6), (6), (6), (6), (6), (6), (4), (4), (4), (4), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Western Blotting (WB)
(414), (376), (262), (118), (58), (57), (39), (37), (34), (31), (24), (23), (20), (19), (10), (6), (6), (5), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
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Product Details anti-IL-4 Antibody

Target Details IL-4 Application Details Handling Images
Specificity This antibody recognises Rat IL-4.
Purification Affinity chromatography.
Immunogen Highly pure (>98%) recombinant rat IL-4 (rat Interleukin-4).

Target Details IL-4

Product Details anti-IL-4 Antibody Application Details Handling Images back to top
Antigen
Alternative Name Interleukin-4 / IL4 (IL4 Antibody Abstract)
Background IL4 is a pleiotropic cytokine produced by activated T cells, mast cells and basophils. It is a ligand for Interleukin 4 receptor. The Interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. IL4 elicits many different biological responses, but has two dominant functions. The first is regulating differentiation of naive CD4+ T cell to the Th2 type. Th2 cells produce IL4, IL5, IL10 and IL13, which tend to favor a humoral immune response while suppressing a cell mediated immune response controlled by Th1 cells. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. The second is regulating IgE and IgG1 production by B cells. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.Synonyms: B-cell stimulatory factor 1, BSF1, Binetrakin, IL-4, Lymphocyte stimulatory factor 1, Pitrakinra
Gene ID 287287
NCBI Accession NP_958427
UniProt P20096
Pathways JAK-STAT Signaling

Application Details

Product Details anti-IL-4 Antibody Target Details IL-4 Handling Images back to top
Application Notes ELISA: To detect rat IL-4 by direct ELISA (using 100 ml/well antibody solution) this antibody can beused at a concentration of 0.15-0.30 μg/mL. Used in conjunction with compatiblesecondary reagents, allows the detection of at least 0.2 ng/well of recombinant rat IL-4. Western Blot: To detect rat IL-4 by Western Blot analysis this antibody can be used at a concentration of0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limitfor recombinant rat IL-4 is 1.5-3.0 ng/lane, under either reducing or non-reducingconditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-IL-4 Antibody Target Details IL-4 Application Details Images back to top
Reconstitution Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.
Buffer PBS, pH 7.2 without preservatives.
Preservative Without preservative
Handling Advice Avoid repeated freezing and thawing. Centrifuge vial prior to opening!
Storage 4 °C/-20 °C
Storage Comment Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.