Rat (Rattus) IL-4 ELISA Kit

Recommended IL-4 Product (supplied by: Log in to see )

Antigen
Interleukin 4 (IL4) ELISA Kits
  • IL4
  • IL-4
  • BCGF-1
  • BCGF1
  • BSF-1
  • BSF1
  • Il-4
  • Il4e12
  • IL2
  • interleukin 4
  • IL4
  • Il4
  • Il-4
Reactivity
Rat (Rattus)
Alternatives
Kits with alternative reactivity to:
75
43
41
20
14
12
11
10
10
10
9
6
5
4
3
2
2
1
1
Method Type
Sandwich ELISA
Detection Range
1.5-160 pg/mL
Minimum Detection Limit
1.5 pg/mL
Application
ELISA
Options
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Catalog No. ABIN625203
$ 476.30
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
12.498861 ABIN416248 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 15.6-1000 pg/mL 15.6 pg/mL Log in to see 3
12.498861 ABIN365190 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Culture Supernatant 15.6-1000 pg/mL 15.6 pg/mL Log in to see 2
12.498861 ABIN411301 Sandwich ELISA Cell Culture Supernatant, Serum 15.6-1000 pg/mL 15.6 pg/mL Log in to see 19
12.498861 ABIN2472110 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant 1.63-100 pg/mL 1.63 pg/mL Log in to see
12.498861 ABIN625204 Sandwich ELISA Cell Lysate, Tissue Lysate Log in to see
12.498861 ABIN1115666 Sandwich ELISA Serum, Plasma, Biological Fluids 15.625-1000 pg/mL 15.625 pg/mL Log in to see 2
12.498861 ABIN1029016 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 78.125-5000 pg/mL 78.125 pg/mL Log in to see
12.498861 ABIN455594 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma, Biological Fluids 15.6-1000 pg/mL 15.6 pg/mL Log in to see
12.498861 ABIN491780 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 1.37-1.000 pg/mL 1.37 pg/mL Log in to see
12.498861 ABIN2708661 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma, Tissue Homogenate 15.625-1000 pg/mL 15.625 pg/mL Log in to see
12.498861 ABIN2708662 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma, Tissue Homogenate 15.6-1000 pg/mL 15.6 pg/mL Log in to see
12.498861 ABIN2114326 Cell Culture Supernatant, Serum Log in to see
1 ABIN4993139 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 0.82-200 pg/mL 0.82 pg/mL Log in to see
1 ABIN627180 Sandwich ELISA Serum, Cell Culture Supernatant, Tissue Homogenate, Plasma, Body Fluids 50-1000 pg/mL 50 pg/mL Log in to see
1 ABIN5592265 Sandwich ELISA Biological Fluids, Plasma, Serum, Tissue Homogenate 15.6-1000 pg/mL 15.6 pg/mL Log in to see
1 ABIN1446155 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma, Biological Samples 15 pg/mL Log in to see
1 ABIN5520978 Sandwich ELISA Plasma, Serum, Biological Fluids, Tissue Homogenate 15.625-1000 pg/mL 15.625 pg/mL Log in to see
1 ABIN1112650 Sandwich ELISA Serum, Body Fluids, Tissue Lysate, Cell Culture Supernatant 7.8-500 pg/mL 7.8 pg/mL Log in to see
1 ABIN955910 Sandwich ELISA 7.8-500 pg/mL 7.8 pg/mL Log in to see
1 ABIN2042554 Sandwich ELISA 15.6-1000 pg/mL 15.6 pg/mL Log in to see

General

Antigen Interleukin 4 (IL4) ELISA Kits
Reactivity Rat (Rattus)
Kits with alternative reactivity to:
(75), (43), (41), (20), (14), (12), (11), (10), (10), (10), (9), (6), (5), (4), (3), (2), (2), (1), (1)
Method Type Sandwich ELISA
Detection Range 1.5-160 pg/mL
Minimum Detection Limit 1.5 pg/mL
Application ELISA
Pubmed 9 references available
Supplier Log in to see

Product Details IL-4 ELISA Kit

Target details Application Details Handling ProductDetails: References for IL-4 Kit (ABIN625203) Images
Purpose Rat IL-4 ELISA Kit for cell culture supernatants, plasma, and serum samples.
Sample Type Plasma, Cell Culture Supernatant, Serum
Detection Method Colorimetric
Analytical Method Quantitative
Specificity This ELISA kit shows no cross-reactivity with any of the cytokines tested: rat CINC-2, CINC-3, CNTF, IL-1 alpha, IL-1 beta, IL-6, IL-10, GM-CSF, IFN-gamma, Leptin, Lix, MCP-1, MIP-3 alpha, beta-NGF, TIMP-1, TNF-alpha, VEGF.
Cross-Reactivity (Details) This ELISA kit shows no cross-reactivity with any of the cytokines tested (e.g., rat CINC-2, CINC-3, CNTF, IL-1alpha, IL-1beta, IL-6, IL- 10, GM-CSF, IFN-gamma, Leptin, Lix, MCP-1, MIP-3alpha, beta-NGF, TIMP-1, TNF-alpha, VEGF)
Sensitivity < 1.5 pg/mL
Characteristics
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data
Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Material not included
  • Distilled or deionized water
  • Precision pipettes to deliver 2 μL to 1 μL volumes
  • Adjustable 1-25 μL pipettes for reagent preparation
  • 100 μL and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis

Target details

Product Details IL-4 ELISA Kit Application Details Handling ProductDetails: References for IL-4 Kit (ABIN625203) Images back to top
Antigen
Alternative Name IL-4 (IL4 ELISA Kit Abstract)
Background Interleukin-4 (IL-4) (B-cell IGG differentiation factor) (B-cell growth factor 1) (B-cell stimulatory factor 1) (BSF-1) (Lymphocyte stimulatory factor 1)
Gene ID 287287
UniProt P20096
Research Area Cytokines, Immunology, Virology, Inflammation, Cancer
Pathways JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation

Application Details

Product Details IL-4 ELISA Kit Target details Handling ProductDetails: References for IL-4 Kit (ABIN625203) Images back to top
Application Notes Recommended Dilution for serum and plasma samples2 fold
Sample Volume 100 μL
Plate Pre-coated
Protocol
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 μL of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4 °C.
  4. Add 100 μL of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 μL of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 μL of Stop Solution to each well.
  11. Read at 450 nm immediately.
Reagent Preparation 1. Bring all reagents and samples to room temperature (18 - 25 °C) before use.
2. Sample dilution: If your samples need to be diluted, Assay Diluent A (Item D) should be used for dilution of serum/plasma samples. 1x Assay Diluent B (Item E) should be used for dilution of culture supernatants. Suggested dilution for normal serum/plasma: 2 fold*. * Please note that levels of the target protein may vary between different specimens. Optimal dilution factors for each sample must be determined by the investigator.
3. Assay Diluent B should be diluted 5-fold with deionized or distilled water.
4. Preparation of standard: Briefly spin the vial of Item C. Add 700 µL Assay Diluent A (for serum/plasma samples) or 1x Assay Diluent B (for cell culture medium) into Item C vial to prepare a 50 ng/mL standard. Dissolve the powder thoroughly by a gentle mix. Add 2 µL IL-4 standard from the vial of Item C, into a tube with 623 µL Assay Diluent A or 1x Assay Diluent B to prepare a 160 pg/mL stock standard solution. Pipette 450 µL Assay Diluent A or 1x Assay Diluent B into each tube. Use the stock standard solution to produce a dilution series . Mix each tube thoroughly before the next transfer. Assay Diluent A or 1x Assay Diluent B serves as the zero standard (0 pg/mL). 300 µL 300myl 300 µL 300 µL 300 µL 300 µL 2 µL standard + 623 µL 160 64 25 10.24 4.10 1.64 0.66 0 pg/mL pg/mL pg/mL pg/mL pg/mL pg/mL pg/mL pg/mL
5. If the Wash Concentrate (20x) (Item B) contains visible crystals, warm to room temperature and mix gently until dissolved. Dilute 20 ml of Wash Buffer Concentrate into deionized or distilled water to yield 400 ml of 1x Wash Buffer.
6. Briefly spin the Detection Antibody vial (Item F) before use. Add 100 µL of 1x Assay Diluent B into the vial to prepare a detection antibody concentrate. Pipette up and down to mix gently (the concentrate can be stored at 4 °C for 5 days). The detection antibody concentrate should be diluted 65-fold with 1x Assay Diluent B and used in step 4 of Part VI Assay Procedure.
7. Briefly spin the HRP-Streptavidin concentrate vial (Item G) and pipette up and down to mix gently before use. HRP-Streptavidin concentrate should be diluted 600-fold with 1x Assay Diluent B. For example: Briefly spin the vial (Item G) and pipette up and down to mix gently . Add 20 µL of HRP-Streptavidin concentrate into a tube with 12 ml 1x Assay Diluent B to prepare a 600-fold diluted HRP-Streptavidin solution (don't store the diluted solution for next day use). Mix well.
Assay Procedure 1. Bring all reagents and samples to room temperature (18 - 25 °C) before use. It is recommended that all standards and samples be run at least in duplicate.
2. Add 100 µL of each standard (see Reagent Preparation step 2) and sample into appropriate wells. Cover well and incubate for 2.5 hours at room temperature or over night at 4 °C with gentle shaking.
3. Discard the solution and wash 4 times with 1x Wash Solution. Wash by filling each well with Wash Buffer (300 myl) using a multi-channel Pipette or autowasher. Complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining Wash Buffer by aspirating or decanting. Invert the plate and blot it against clean paper towels.
4. Add 100 µL of 1x prepared biotinylated antibody (Reagent Preparation step 6) to each well. Incubate for 1 hour at room temperature with gentle shaking.
5. Discard the solution. Repeat the wash as in step
6. Add 100 µL of prepared Streptavidin solution (see Reagent Preparation step 7) to each well. Incubate for 45 minutes at room temperature with gentle shaking.
7. Discard the solution. Repeat the wash as in step
8. Add 100 µL of TMB One-Step Substrate Reagent (Item H) to each well. Incubate for 30 minutes at room temperature in the dark with gentle shaking.
9. Add 50 µL of Stop Solution (Item I) to each well. Read at 450 nm immediately.
Calculation of Results Calculate the mean absorbance for each set of duplicate standards, controls and samples, and subtract the average zero standard optical density. Plot the standard curve on log-log graph paper or using Sigma plot software, with standard concentration on the x-axis and absorbance on the y-axis. Draw the best-fit straight line through the standard points.
Typical Data: These standard curves are for demonstration only. A standard curve must be run with each assay. Assay Diluent A Rat IL-4 concentration (pg/mL) 0.1 1 10 100 1000 O D =4 50 n m 0.01 0.1 1 10 Assay Diluent B Rat IL-4 concentration (pg/mL) 0.1 1 10 100 1000 O D =4 50 n m 0.01 0.1 1 10
Sensitivity: The minimum detectable dose of IL-4 is typically less than 1.5 pg/mL.
Recovery: Recovery was determined by spiking various levels of rat IL-4 into rat serum, plasma and cell culture media. Mean recoveries are as follows: Sample Type Average % Recovery Range ( %) Serum 96.79 84-103 Plasma 94.86 82-102 Cell culture media 97.69 85-104
Linearity: Sample Type Serum Plasma Cell Culture Media 1:2 Average % of Expected 93 94 96 Range ( %) 82-102 84-103 85-105 1:4 Average % of Expected 97 98 97 Range ( %) 85-105 86-105 86-106
Reproducibility: Intra-Assay: CV<10 % Inter-Assay: CV<12 %
Assay Precision Intra-Assay: CV< 10 % Inter-Assay: CV< 12 %
Restrictions For Research Use only

Handling

Product Details IL-4 ELISA Kit Target details Application Details ProductDetails: References for IL-4 Kit (ABIN625203) Images back to top
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Expiry Date 6 months

ProductDetails: References for IL-4 Kit (ABIN625203)

Product Details IL-4 ELISA Kit Target details Application Details Handling Images back to top
Product cited in:

Lin, Zhang, Dai, Li, Wang, Zhang, Chen, Lu: "Expression of interleukin-9 and its upstream stimulating factors in rats with ischemic stroke." in: Neurological sciences : official journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology, 2015

Zhang, Zhang, Tsui: "Mesenteric lymph duct drainage attenuates acute lung injury in rats with severe intraperitoneal infection." in: Inflammation, Vol. 38, Issue 3, pp. 1239-49, 2015

Choudhary, Sheela Devi: "Longer period of oral administration of aspartame on cytokine response in Wistar albino rats." in: Endocrinologi?a y nutricio?n : o?rgano de la Sociedad Espan?ola de Endocrinologi?a y Nutricio?n, Vol. 62, Issue 3, pp. 114-22, 2015

Ohtani, Ushiyama, Maeda, Ogasawara, Wang, Kunugita, Ishii: "The effects of radio-frequency electromagnetic fields on T cell function during development." in: Journal of radiation research, Vol. 56, Issue 3, pp. 467-74, 2015

Arafa, Mohammad, Atteia: "Fenugreek seed powder mitigates cadmium-induced testicular damage and hepatotoxicity in male rats." in: Experimental and toxicologic pathology : official journal of the Gesellschaft für Toxikologische Pathologie, Vol. 66, Issue 7, pp. 293-300, 2014

Arafa, Mohammad, Atteia: "Fenugreek seed powder mitigates cadmium-induced testicular damage and hepatotoxicity in male rats." in: Experimental and toxicologic pathology : official journal of the Gesellschaft für Toxikologische Pathologie, Vol. 66, Issue 7, pp. 293-300, 2014

Koshy, Huq, Tanner, Atik, Porter, Khan, Pennington, Hanania, Corry, Beeton: "Blocking KV1.3 channels inhibits Th2 lymphocyte function and treats a rat model of asthma." in: The Journal of biological chemistry, Vol. 289, Issue 18, pp. 12623-32, 2014

Resnick, Blum, Bose, Smith, Toogood: "Characteristics of unmarried adolescent mothers: determinants of child rearing versus adoption." in: The American journal of orthopsychiatry, Vol. 60, Issue 4, pp. 577-84, 1991

Wright: "Respiratory syncytial virus infection." in: Journal of the Iowa Medical Society, Vol. 55, Issue 6, pp. 301-9, 1970

Images

Product Details IL-4 ELISA Kit Target details Application Details Handling ProductDetails: References for IL-4 Kit (ABIN625203) back to top
Supplier Images
ELISA image for Interleukin 4 (IL4) ELISA Kit (ABIN625203) Interleukin 4 (IL4) ELISA Kit