IL-4 ELISA Kit

Catalog No. ABIN411300

The Mouse IL-4 ELISA Kit (ABIN411300) is a Colorimetric ELISA Kit designed to quantify Mouse IL-4. This ELISA Kit has been cited in 31 publications.

Quick Overview for IL-4 ELISA Kit (ABIN411300)

Target: IL-4 (IL4) (Interleukin 4 (IL4))

Binding Specificity: AA 21-140

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 7.8 pg/mL - 500 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Serum

Product Details IL-4 ELISA Kit

Minimum Detection Limit: 7.8 pg/mL

Purpose: Sandwich Mouse IL-4/Interleukin-4 ELISA Kit to quantitate Mouse Il4 in cell culture supernatants and serum.

Analytical Method: Quantitative

Specificity: Expression system for standard: E.coli
Immunogen sequence: H21-S140

Capture antibody and Detection antibody: monoclonal antibody from rat, polyclonal antibody from goat

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 1 pg/mL

Components:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Material not included: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Sample Volume: 100 μL

Plate: Pre-coated

Protocol: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Assay Precision: Intra-Assay Precision (CV%): 7.4%, 6.9%, 7.3%
Inter-Assay Precision (CV%): 9.3%, 7.2%, 7.6%

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Expiry Date: 12 months

References for IL-4 ELISA Kit (ABIN411300)

Li, Jin, Wang, Li, Liu, Chang, Wang, Liu: "Live attenuated Salmonella displaying HIV-1 10E8 epitope on fimbriae: systemic and mucosal immune responses in BALB/c mice by mucosal administration." in: Scientific reports, Vol. 6, pp. 29556, (2018) (PubMed).

Dong, Han, Wang, Qin, Yang, Cao, Chen: "Role of serotonin on the intestinal mucosal immune response to stress-induced diarrhea in weaning mice." in: BMC gastroenterology, Vol. 17, Issue 1, pp. 82, (2018) (PubMed).

Han, Zhang, Tan, Liu, Ma, Ou-Yang, Wu: "EPO modified MSCs can inhibit asthmatic airway remodeling in an animal model." in: Journal of cellular biochemistry, Vol. 119, Issue 1, pp. 1008-1016, (2018) (PubMed).

Yang, Zhang, Cai, Shang: "Epigallocatechin-3-gallate inhibits inflammation and epithelial‑mesenchymal transition through the PI3K/AKT pathway via upregulation of PTEN in asthma." in: International journal of molecular medicine, Vol. 41, Issue 2, pp. 818-828, (2018) (PubMed).

Hu, Feng, Wang, Song, Ni: "5-BDBD ameliorates an OVA-induced allergic asthma by the reduction of Th2 cytokines production." in: Iranian journal of basic medical sciences, Vol. 21, Issue 4, pp. 364-369, (2018) (PubMed).

Zhang, Li, Wang, Xie, Li, Zuo, Kong, Wang, Wang: "Immune Protection of Rhoptry Protein 21 (ROP21) of Toxoplasma gondii as a DNA Vaccine Against Toxoplasmosis." in: Frontiers in microbiology, Vol. 9, pp. 909, (2018) (PubMed).

Wang, Zhang, Wang, Gadahi, Xu, Yan, Song, Li: "Toxoplasma gondii Elongation Factor 1-Alpha (TgEF-1α) Is a Novel Vaccine Candidate Antigen against Toxoplasmosis." in: Frontiers in microbiology, Vol. 8, pp. 168, (2017) (PubMed).

Liu, Huang, Yao, Yang: "The Immunotherapeutic Role of Bacterial Lysates in a Mouse Model of Asthma." in: Lung, (2017) (PubMed).

Wang, Su, Dong, Shi, Gao, Wang: "Hybrid phage displaying SLAQVKYTSASSI induces protection against Candida albicans challenge in BALB/c mice." in: Human vaccines & immunotherapeutics, Vol. 10, Issue 4, pp. 1057-63, (2016) (PubMed).

Wang, Wang, Sun, Zhang, Liu, Gadahi, Hassan, Xu, Yan, Song, Li: "Protective immunity against acute toxoplasmosis in BALB/c mice induced by a DNA vaccine encoding Toxoplasma gondii elongation factor 1-alpha." in: BMC infectious diseases, Vol. 15, pp. 448, (2016) (PubMed).

Zhang, Liang, Li, Wang, Zhang, Xu, Huo, Cao, Zhong, Zhong: "Mutual enhancement of IL-2 and IL-7 on DNA vaccine immunogenicity mainly involves regulations on their receptor expression and receptor-expressing lymphocyte generation." in: Vaccine, Vol. 33, Issue 30, pp. 3480-7, (2015) (PubMed).

Presti, DOrazio, Labra, La Ferla, Mezzasalma, Bizzaro, Giardina, Michelotti, Tursi, Vassallo, Di Gennaro: "Evaluation of the probiotic properties of new Lactobacillus and Bifidobacterium strains and their in vitro effect." in: Applied microbiology and biotechnology, Vol. 99, Issue 13, pp. 5613-26, (2015) (PubMed).

Huang, Wang, Li, Ge, Chen, Chen: "Trichostatin A reduces cisplatin-induced ototoxicity through the STAT6 signaling pathway." in: International journal of molecular medicine, Vol. 36, Issue 2, pp. 493-500, (2015) (PubMed).

Li, Hu, Wang, Wu, Zhang: "Different profiles of notch signaling in cigarette smoke-induced pulmonary emphysema and bleomycin-induced pulmonary fibrosis." in: Inflammation research : official journal of the European Histamine Research Society ... [et al.], Vol. 64, Issue 5, pp. 363-71, (2015) (PubMed).

Qiu, Zhu, Lu, Bai, Liu, Zhao, Sun, Zhang, Li, Liu: "The selective mGluR5 agonist CHPG attenuates SO2-induced oxidative stress and inflammation through TSG-6/NF-?B pathway in BV2 microglial cells." in: Neurochemistry international, Vol. 85-86, pp. 46-52, (2015) (PubMed).

Hassan, Wang, Xu, Yan, Song, Li: "DNA vaccination with a gene encoding Toxoplasma gondii Deoxyribose Phosphate Aldolase (TgDPA) induces partial protective immunity against lethal challenge in mice." in: Parasites & vectors, Vol. 7, pp. 431, (2015) (PubMed).

Li, Han, Liang, Zhong, Li, Wen, Lin, Wang, Li, Zhong, Zhong: "Molecular mechanism for the effects of E. coli heat-labile enterotoxin on mouse embryo survival." in: Reproductive toxicology (Elmsford, N.Y.), Vol. 45, pp. 31-8, (2014) (PubMed).

Ozkan, Deveci, Kumbak, Simsek, Ilhan, Sekercioglu, Sapmaz: "What is the impact of Th1/Th2 ratio, SOCS3, IL17, and IL35 levels in unexplained infertility?" in: Journal of reproductive immunology, Vol. 103, pp. 53-8, (2014) (PubMed).

Ou, Shi, Xu, Tao: "Intranasal immunization with DNA vaccine coexpressing Der p 1 and ubiquitin in an allergic rhinitis mouse model." in: Annals of allergy, asthma & immunology : official publication of the American College of Allergy, Asthma, & Immunology, Vol. 113, Issue 6, pp. 658-665.e1, (2014) (PubMed).

Jin, Cheng, Liu, Wu, Wang, Wei, Zhou, Qin, Jia, Zhen et al.: "Improvement of functional recovery by chronic metformin treatment is associated with enhanced alternative activation of microglia/macrophages and increased angiogenesis and neurogenesis following ..." in: Brain, behavior, and immunity, Vol. 40, pp. 131-42, (2014) (PubMed).

Target Details for IL-4

Target: IL-4 (IL4) (Interleukin 4 (IL4))

Alternative Name: IL4

Background:

Background: Interleukin-4 (IL-4), also knowns as a B-cell stimulatory factor1 (BSF1), is an immunomodulatory cytokine, which can inhibit the growth of tumour cells. The human cDNA contains a single open reading frame encoding a protein of 153 amino acids, including a putative signal peptide. IL-4 may act as an autocrine growth factor in pancreatic cancer cells and also give rise to the possibility that cancer-derived IL-4 may suppress cancer-ed immunosurveillance in vivo in addition to its growth-promoting effects, thereby facilitating pancreatic tumor growth and metastasis.1 The mouse and human genes and their protein products show structural and functional similarities. The human IL-4 gene, which occurs as a single copy in the haploid genome, is mapped on chromosome 5.

Gene Full Name: interleukin 4

Gene ID: 16189

UniProt: P07750

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation