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anti-Mouse (Murine) IL-6 Receptor antibody for Blocking Reagent

Recommended IL-6 Receptor Antibody (supplied by: Log in to see )

Antigen
Interleukin-6 Receptor (IL6R) Antibodies
  • CD126
  • gp80
  • IL-6R
  • IL-6R-1
  • IL-6RA
  • il-6ra
  • IL-6Ralpha
  • IL6Q
  • Il6r
  • IL6R
  • IL6R1
  • IL6RA
  • Il6ra
  • IL6RQ
Reactivity
Mouse (Murine)
153
64
44
1
Host
Rat
105
88
29
15
1
Clonality
Monoclonal
Conjugate
This IL-6 Receptor antibody is un-conjugated
22
14
12
10
5
4
3
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Blocking Reagent (BR), Functional Studies (Func)
100
100
80
42
36
26
25
23
18
12
11
9
8
6
4
2
2
1
1
1
1
Supplier
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Catalog No. ABIN1177015
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008711731 ABIN438583 BR FACS IP Neut Rat IgG2 Log in to see AP-MAB0848

Top referenced anti-IL-6 Receptor antibody for Blocking Reagent

Similar anti-IL-6 Receptor Antibodies

Application / Reactivity Mouse (Murine)
Blocking Reagent (BR) 2 Antibodies
ELISA 16 Antibodies
ELISA (Capture) 4 Antibodies
ELISpot (Capture) (ELISPOT (Capture)) 2 Antibodies
Flow Cytometry (FACS) 22 Antibodies
Functional Studies (Func) 2 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 2 Antibodies
Immunofluorescence (IF) 3 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 7 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 5 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 5 Antibodies
Immunoprecipitation (IP) 2 Antibodies
Intracellular Flow Cytometry (ICFC) 4 Antibodies
Neutralization (Neut) 3 Antibodies
Western Blotting (WB) 19 Antibodies

General

Antigen Interleukin-6 Receptor (IL6R) Antibodies
Reactivity Mouse (Murine)
(153), (64), (44), (1)
Host Rat
(105), (88), (29), (15), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This IL-6 Receptor antibody is un-conjugated
(22), (14), (12), (10), (5), (4), (3), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Blocking Reagent (BR), Functional Studies (Func)
(100), (100), (80), (42), (36), (26), (25), (23), (18), (12), (11), (9), (8), (6), (4), (2), (2), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-IL-6 Receptor Antibody

Target Details IL-6 Receptor Application Details Handling References for anti-IL-6 Receptor Antibody (ABIN1177015) Images
Characteristics Neutralization Activity: 50% Neutralization (ND50) at 1.0 - 6.0 myg/mL
>95% Neutralization at 10 - 50 myg/ml
Purification Purified from tissue culture supernatant or ascites by affinity chromatography
Sterility 0.2 μm filtered
Endotoxin Level ≤0.01 ng/µg
Immunogen OKT4 hybridoma cells
Clone D7715A7
Isotype IgG2b kappa

Target Details IL-6 Receptor

Product Details anti-IL-6 Receptor Antibody Application Details Handling References for anti-IL-6 Receptor Antibody (ABIN1177015) Images back to top
Antigen
Alternative Name CD126 (IL6R Antibody Abstract)
Background The D7715A7 antibody reacts with the mouse IL-6 receptor. The immunogen used to generate the D7715A7 hybridoma was OKT4 hybridoma cells. The D7715A7 hybridoma was selected based on its capacity to produce antibody that inhibited IL-6 binding to 2F4 cells (mouse B-cell hybridoma expressing high levels of IL-6 receptors). The binding of purified D7715A7 to B9 cells (mouse B-cell hybridoma expressing high levels of IL-6 receptors) is inhibited by recombinant mouse IL-6. This antibody has been reported to inhibit the in vitro and in vivo growth of the IL-6-dependent plasmacytoma line, T1033C2.
Synonyms: IL-6 Receptor alpha chain
Pathways JAK-STAT Signaling

Application Details

Product Details anti-IL-6 Receptor Antibody Target Details IL-6 Receptor Handling References for anti-IL-6 Receptor Antibody (ABIN1177015) Images back to top
Application Notes In vitro neutralization: The D7715A7 antibody is useful in neutralization of mouse IL-6 bioactivity Indicator cell: NFS-60 at 4 x 10^4 cells/ml Assay Type: Proliferation at 1 ng/ml mouse IL-6 (Cat No. 554582) Preincubation of antibody and ligand = 60 minutes
Immunofluorescent Staining: For optimal staining the D7715A7 antibody (≤1 µg/million cells) should be used in conjunction with biotinylated goat anti-rat Ig with no cross-reactivity to mouse Ig (Cat. No. 554014) and streptavidin-phycoerythrin (Cat. No. 554061) in a three-step staining procedure to amplify immunofluorescent signals for the flow cytometric analysis of cytokine receptor-expressing cells.
Restrictions For Research Use only

Handling

Product Details anti-IL-6 Receptor Antibody Target Details IL-6 Receptor Application Details References for anti-IL-6 Receptor Antibody (ABIN1177015) Images back to top
Format Liquid
Concentration 1.0 mg/ml
Buffer Aqueous buffered solution
Preservative Azide free
Storage 4 °C

References for anti-IL-6 Receptor Antibody (ABIN1177015)

Product Details anti-IL-6 Receptor Antibody Target Details IL-6 Receptor Application Details Handling Images back to top
Product cited in:

Coulie, Vink, Van Snick: "A monoclonal antibody specific for the murine IL-6-receptor inhibits the growth of a mouse plasmacytoma in vivo." in: Current topics in microbiology and immunology, Vol. 166, pp. 43-6, 1991

Vink, Coulie, Warnier, Renauld, Stevens, Donckers, Van Snick: "Mouse plasmacytoma growth in vivo: enhancement by interleukin 6 (IL-6) and inhibition by antibodies directed against IL-6 or its receptor." in: The Journal of experimental medicine, Vol. 172, Issue 3, pp. 997-1000, 1990