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|Antigen||Interleukin-6 Receptor (IL6R) Antibodies|
|Epitope||Extracellular Domain Alternatives|
|Reactivity||Mouse (Murine) Alternatives|
|Conjugate||This IL-6 Receptor antibody is un-conjugated Alternatives|
Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
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Product Details anti-IL-6 Receptor AntibodyTarget Details IL-6 Receptor Application Details Handling Images
|Specificity||This antibody detects Mouse IL-6R. Other species not tested.|
|Purification||Protein A/G affinity chromatography|
|Immunogen||Recombinant protein of IL-6R extracellular domain.|
Target Details IL-6 ReceptorProduct Details anti-IL-6 Receptor Antibody Application Details Handling Images back to top
|Alternative Name||CD126 / IL6R (IL6R Antibody Abstract)|
|Background||IL6 exerts its activity through binding to a high affinity receptor complex consisting of two membrane glycoproteins: an 80 kDa IL6 binding receptor protein (IL6R) and a 130 kDa signal transducing protein consisting of a 19 amino acid residue signal peptide (gp130). Human IL6R is a 468 amino acid residue type I membrane protein consisting of a 19 amino acid residue signal peptide, a 339 residue extracellular region, a 28 residue transmembrane region and an 82 residue cytoplasmic region. The ectodomain of IL6R is made up of an immunoglobulin like domain and a cytokine receptor superfamily domain. A naturally occurring soluble form of the IL6R, which binds IL6 and which mediates IL6 signaling through interaction with gp130, is identified in the urine of healthy adult humans. It has also been detected in human and mouse serum as well as in the conditioned medium of various cells including human peripheral blood mononuclear cells, and several T cell or granulocyte/macrophage cell lines. Increased expression of the c-fos and c-myc protooncogenes have been linked to IL6 receptor activation.Synonyms: IL-6R 1, IL-6R-alpha, IL6 Receptor alpha, Interleukin-6 receptor subunit alpha, Membrane glycoprotein 80|
|Research Area||CD Antigens, Surface Receptors of Immune Cells|
|Pathways||JAK-STAT Signaling, Autophagy, Growth Factor Binding|
Application DetailsProduct Details anti-IL-6 Receptor Antibody Target Details IL-6 Receptor Handling Images back to top
Western Blot: 1/500 - 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-IL-6 Receptor Antibody Target Details IL-6 Receptor Application Details Images back to top
|Reconstitution||Restore with 0.2 mL sterile PBS and the final concentration is 0.5 mg/mL.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|