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Human IL-6 Receptor ELISA Kit

Recommended IL-6 Receptor Product (supplied by: Log in to see )

Antigen
Interleukin-6 Receptor (IL6R) ELISA Kits
  • CD126
  • gp80
  • IL-6R
  • IL-6R-1
  • IL-6RA
  • il-6ra
  • IL-6Ralpha
  • IL6Q
  • Il6r
  • IL6R
  • IL6R1
  • IL6RA
  • Il6ra
  • IL6RQ
Epitope
AA 20-358
Reactivity
Human
Alternatives
Kits with alternative reactivity to:
21
9
7
3
2
2
2
2
1
1
1
1
1
Methode Type
Sandwich ELISA
Detection Range
31.2-2000 pg/mL
Minimum Detection Limit
31.2 pg/mL
Application
ELISA
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Catalog No. ABIN1672798
$ 369.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
0.32693866 ABIN417288 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Urine, Biological Fluids 1.88-120 ng/mL 1.88 ng/mL Log in to see 1
0.32693866 ABIN1379979 Sandwich ELISA Cell Lysate, Serum, Plasma 16-1000 pg/mL 16 pg/mL Log in to see
0.32693866 ABIN2708117 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma, Urine 31.2-2000 pg/mL 31.2 pg/mL Log in to see
0.0012117105 ABIN857562 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant, Tissue Homogenate, Urine 37.5-2400 pg/mL 37.5 pg/mL Log in to see 1
0.0012117105 ABIN3198462 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 15.63-1000 pg/mL 15.63 pg/mL Log in to see
0.0012117105 ABIN1055311 Serum, Cell Culture Supernatant, Body Fluids, Tissue Homogenate, Plasma Log in to see 1
0.0012117105 ABIN2684723 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 31.2-2000 pg/mL 31.2 pg/mL Log in to see 3
0.0012117105 ABIN1446007 Cell Culture Supernatant, Serum, Plasma, Biological Samples < 5.0pg/mL Log in to see
0.0012117105 ABIN2748263 Sandwich ELISA Plasma, Serum, Cell Culture Supernatant, Cell and Tissue Culture Lysate, Body Fluids 12-3000 pg/mL Log in to see
0.0012117105 ABIN2684722 Sandwich ELISA Plasma (EDTA), Plasma (heparin), Amniotic Fluid, Cell Culture Supernatant, Serum, Urine 0.08-5 ng/mL 0.08 ng/mL Log in to see
0.0012117105 ABIN2684724 Sandwich ELISA Plasma (EDTA), Plasma (heparin), Cell Culture Supernatant, Saliva, Serum, Urine 31.2-2000 pg/mL 31.2 pg/mL Log in to see
0.0012117105 ABIN2042560 Sandwich ELISA 31.2-2000 pg/mL 31.2 pg/mL Log in to see
0.0012117105 ABIN2114331 Cell Lysate, Serum, Plasma 16-1000 pg/mL 16 pg/mL Log in to see
0.0012117105 ABIN2851285 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 1.875-120 ng/mL 1.875 ng/mL Log in to see
0.0012117105 ABIN585293 62.5-4000 pg/mL 62.5 pg/mL Log in to see
0.0012117105 ABIN1033788 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma (EDTA), Plasma (heparin), Amniotic Fluid, Urine Log in to see
0.0012117105 ABIN2869886 Log in to see
0.0012117105 ABIN2535634 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Urine, Biological Fluids 1.88-120 ng/mL 1.88 ng/mL Log in to see
0.0012117105 ABIN2088997 Sandwich ELISA Log in to see
0.0012117105 ABIN2948915 62.5-4000 pg/mL 62.5 pg/mL Log in to see

Top referenced IL-6 Receptor ELISA Kit for ELISA

Similar IL-6 Receptor ELISA Kits

Application / Reactivity Human
ELISA 22 ELISA Kits

General

Antigen Interleukin-6 Receptor (IL6R) ELISA Kits
Epitope AA 20-358
Reactivity Human
Kits with alternative reactivity to:
(21), (9), (7), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Methode Type Sandwich ELISA
Detection Range 31.2-2000 pg/mL
Minimum Detection Limit 31.2 pg/mL
Application ELISA
Pubmed 1 reference available
Supplier Log in to see

Product Details IL-6 Receptor ELISA Kit

Target details Application Details Handling References for IL-6 Receptor Kit (ABIN1672798) Images
Purpose For quantitative detection of human IL-6R alpha in cell culture supernates, serum, plasma(heparin, EDTA, citrate) and urine.
Brand PicoKine™
Sample Type Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate), Urine
Detection Method Colorimetric
Specificity Natural and recombinant human IL-6R alpha
Cross-Reactivity (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivity <10pg/mL
Components
  • 96-well plate precoated with anti- human IL-6R alpha antibod - 1
  • Lyophilized recombinant human IL-6R alpha standar - 10ng/tubex2
  • Biotinylated anti- human IL-6R alpha antibod - 130μl(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex (ABC - 130μl(dilution 1:100)
  • Sample diluent buffe - 30 ml
  • Antibody diluent buffe - 12ml
  • ABC diluent buffe - 12ml
  • TMB color developing agen - 10ml
  • TMB stop solutio - 10ml
Material not included Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: sf21
Immunogen sequence: L20-D358

Target details

Product Details IL-6 Receptor ELISA Kit Application Details Handling References for IL-6 Receptor Kit (ABIN1672798) Images back to top
Antigen
Alternative Name IL6R (IL6R ELISA Kit Abstract)
Background IL6R alpha(IL6RA) also known as CD126 or IL6R, is a type I cytokine receptor. The IL6 receptor is a protein complex consisting of a IL-6 receptor subunit(IL6R) and interleukin 6 signal transducer Glycoprotein 130. IL6R also denotes the human gene encoding this subunit. Alternatively spliced transcript variants encoding distinct isoforms have been reported. IL6R subunit is also shared by many other cytokines. Interleukin-6 receptor has been shown to interact with Interleukin 6 and Ciliary neurotrophic factor. Ligand binding did not appear to affect IL6R dimerization status. IL6R dimerization occurs both on the cell surface and in solution.
Gene ID 3570
UniProt A0N0L5
Research Area Stem Cells, Hematopoietic Progenitors, Innate Immunity, Cytokines, Immunology
Pathways JAK-STAT Signaling

Application Details

Product Details IL-6 Receptor ELISA Kit Target details Handling References for IL-6 Receptor Kit (ABIN1672798) Images back to top
Application Notes Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Plate Pre-coated,Strips (12 x 8)
Protocol human IL-6R alpha ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-6R alpha has been precoated onto 96-well plates. Standards(sf21, L20-D358) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-6R alpha is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL-6R alpha amount of sample captured in plate.
Assay Procedure Aliquot 0.1 mL per well of the 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human IL-6R alpha standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA, citrate) or urine to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human IL-6R alpha standard solution and each sample be measured in duplicate.
Assay Precision
  • Sample 1: n=16, Mean(pg/ml): 115, Standard deviation: 9.2, CV(%): 8
  • Sample 2: n=16, Mean(pg/ml): 768, Standard deviation: 26.11, CV(%): 3.4
  • Sample 3: n=16, Mean(pg/ml): 1558, Standard deviation: 60.76, CV(%): 3.9,
  • Sample 1: n=24, Mean(pg/ml): 124, Standard deviation: 10.54, CV(%): 8.5
  • Sample 2: n=24, Mean(pg/ml): 845, Standard deviation: 47.32, CV(%): 5.6
  • Sample 3: n=24, Mean(pg/ml): 1334, Standard deviation: 81.37, CV(%): 6.1
Restrictions For Research Use only

Handling

Product Details IL-6 Receptor ELISA Kit Target details Application Details References for IL-6 Receptor Kit (ABIN1672798) Images back to top
Handling Advice Avoid multiple freeze-thaw cycles.
Storage -20 °C,4 °C
Storage Comment Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date 12 months

References for IL-6 Receptor Kit (ABIN1672798)

Product Details IL-6 Receptor ELISA Kit Target details Application Details Handling Images back to top
Background publications

Schuster, Meinert, Rose-John, Kallen: "The human interleukin-6 (IL-6) receptor exists as a preformed dimer in the plasma membrane." in: FEBS letters, Vol. 538, Issue 1-3, pp. 113-6, 2003

Images

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Supplier Images
ELISA image for IL-6 Receptor ELISA Kit (Interleukin-6 Receptor) (ABIN1672798) Human IL-6R alpha PicoKine ELISA Kit standard curve