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|Application / Reactivity||Human|
|Blocking Antibody (Inhibition)||4 Antibodies|
|Blocking Peptide (BP)||1 Antibodies|
|Enzyme Immunoassay (EIA)||6 Antibodies|
|Flow Cytometry (FACS)||9 Antibodies|
|Functional Studies (Func)||4 Antibodies|
|Immunocytochemistry (ICC)||1 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||11 Antibodies|
|Immunohistochemistry (IHC)||26 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||1 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||6 Antibodies|
|Neutralization (Neut)||21 Antibodies|
|Western Blotting (WB)||64 Antibodies|
|Antigen||Interleukin 11 (IL11) Antibodies|
|Conjugate||This IL11 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Functional Studies (Func), Western Blotting (WB)
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Product Details anti-IL11 AntibodyTarget Details IL11 Application Details Handling Images
|Specificity||This antibody detects Human Interleukin-11. Other species not tested.|
|Characteristics||Synonyms: IL-11, Adipogenesis inhibitory factor, AGIF|
|Purification||Affinity Chromatography on Protein A|
|Immunogen||Highly pure (>98%) recombinant Human IL-11|
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|Alternative Name||Interleukin-11 / IL11 (IL11 Antibody Abstract)|
|Background||Interleukin 11 is a pleiotropic cytokine produced by mesenchymal-derived adherent cells. IL11 shares many functions of IL6 and LIF, including potentiation of megakaryocyte activity, enhancement of human myeloma cell proliferation, and enhancement of hepatic acute phase protein production. Interleukin 11 directly stimulates the proliferation of hematopoietic stem cells and megakaryocyte progenitor cells, and induces megakaryocyte maturation resulting in increased platelet production. Clinically it is used to prevent severe thrombocytopenia and the reduction of the need for platelet transfusion following myelosuppressive chemotherapy.Synonyms: AGIF, Adipogenesis inhibitory factor, IL-11|
Application DetailsProduct Details anti-IL11 Antibody Target Details IL11 Handling Images back to top
Neutralisation: To yield one-half maximal inhibition [ND50] of the biological activity ofhIL-11 (5.0 ng/mL), a concentration of 2.4-3.6 μg/mL is required. ELISA: In a sandwich ELISA (assuming 100 μL/well), a concentration of 8-9 μg/mL will detectat least 0.2 ng/well of recombinant Human IL-11 when used with Biotin anti-Human IL-11antibody (cat. PP1033B) as the detection antibody at a concentration of at least 2 μg/mL. Western Blot: To detect hIL-11 by Western Blot analysis this antibody can be used at aconcentration of 1.0-2.0 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hIL-11 is 0.25-0.50 ng/lane, under non-reducingconditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-IL11 Antibody Target Details IL11 Application Details Images back to top
|Reconstitution||Restore in sterile water to a concentration of 1.0 mg/mL.|
|Buffer||PBS without preservatives|
|Storage||4 °C/-20 °C|
The lyophilized antibody is stable at room temperature for one month or at -20 °C forlonger. Following reconstitution it is stable at 2 - 8 °C for six weeks. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
|Expiry Date||12 months|
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