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|Application / Reactivity||Sheep (Ovine)||Human|
|ELISA||17 Antibodies||82 Antibodies|
|ELISpot||1 Antibodies||1 Antibodies|
|Enzyme Immunoassay (EIA)||2 Antibodies||5 Antibodies|
|Flow Cytometry (FACS)||2 Antibodies||22 Antibodies|
|Functional Studies (Func)||2 Antibodies||3 Antibodies|
|Immunohistochemistry (IHC)||1 Antibodies||36 Antibodies|
|Western Blotting (WB)||18 Antibodies|
|Dot Blot (DB)||5 Antibodies|
|ELISA (Capture)||1 Antibodies|
|Immunoassay (IA)||2 Antibodies|
|Immunofluorescence (IF)||1 Antibodies|
|Immunohistochemistry (Acetone-fixed) (IHC (af))||1 Antibodies|
|Antigen||Interleukin 12b (IL12B) Antibodies|
|Reactivity||Cow (Bovine), Human, Sheep (Ovine) Alternatives|
|Conjugate||This IL12B antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Functional Studies (Func), Flow Cytometry (FACS)
|Supplier||Log in to see|
Product Details anti-IL12B AntibodyTarget Details IL12B Application Details Handling Images
|Specificity||This antibody recognizes Interleukin-12. It has been shown to block the biological activity of IL-12.|
|Characteristics||Synonyms: Interleukin-12B, IL-12B, IL-12 subunit p40, CLMF p40, NKSF2, Cytotoxic lymphocytematuration factor 40 kDa subunit, NK cell stimulatory factor chain 2|
|Purification||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant|
|Immunogen||Recombinant bovine IL-12|
Target Details IL12BProduct Details anti-IL12B Antibody Application Details Handling Images back to top
|Alternative Name||Interleukin-12 beta / IL12B (IL12B Antibody Abstract)|
|Background||Interleukin 12 (IL12) can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine activated killer cells, and stimulate the production of IFN gamma by resting PBMC. IL12 induces IFN gamma, TNF alpha production in T and NK cells, costimulation of PBL proliferation, proliferation/differentiation of TH1 T lymphocytes. It is downregulated by IL10, homodimeric p40 antagonistic to functional p70 heterodimer. IL12R beta 1 binds p40 but when dimeric with IL12R beta 2 binds p35.Synonyms: CLMF p40, Cytotoxic lymphocyte maturation factor 40 kDa subunit, IL-12 subunit p40, IL-12B, Interleukin-12B, NK cell stimulatory factor chain 2, NKSF2|
Application DetailsProduct Details anti-IL12B Antibody Target Details IL12B Handling Images back to top
ELISA: 1/100 - 1/1000. Flow cytometry: 1/1000, use 10 μL to label 10^6 cells of 100 l. Functional assays: 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-IL12B Antibody Target Details IL12B Application Details Images back to top
|Buffer||PBS, pH 7.4, None, None|
Store the antibody undiluted at -20 °C. Avoid repeated freezing and thawing.
Shelf life: One year from despatch.
|Expiry Date||12 months|