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anti-Human IL12B antibody for Enzyme Immunoassay

Recommended IL12B Antibody (supplied by: Log in to see )

Antigen
Interleukin 12b (IL12B) Antibodies
  • CLMF
  • CLMF2
  • IL-12
  • IL-12.p40
  • IL-12B
  • Il-12b
  • IL-12p40
  • Il-12p40
  • Il12
  • IL12B
  • IL12p40
  • Il12p40
  • NKSF
  • NKSF2
  • p40
Epitope
Internal Region
28
15
13
12
11
7
5
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Human
126
58
27
19
6
4
2
2
1
1
Host
Goat
127
41
33
11
6
1
1
1
Clonality
Polyclonal
Conjugate
This IL12B antibody is un-conjugated
21
16
14
7
6
6
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
153
140
38
23
14
9
6
5
4
4
4
3
2
1
1
1
1
Supplier
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Catalog No. ABIN374765
$ 379.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008833851 ABIN952920 EIA FACS IHC (p) WB Rabbit Ig AA 271-298, C-Term Log in to see Polyclonal 2
0.0008833851 ABIN118938 EIA IHC (fro) Mouse IgG1 Log in to see 1-1A4
0.0008833851 ABIN317391 EIA Func FACS Mouse IgG2b Log in to see CC326
0.0008833851 ABIN1107794 ELISpot Func EIA FACS WB Mouse IgG2a Log in to see CC301

Top referenced anti-IL12B antibody for Enzyme Immunoassay

Similar anti-IL12B Antibodies

Application / Reactivity Human
Dot Blot (DB) 5 Antibodies
ELISA 82 Antibodies
ELISA (Capture) 1 Antibodies
ELISpot 1 Antibodies
Enzyme Immunoassay (EIA) 5 Antibodies
Flow Cytometry (FACS) 22 Antibodies
Functional Studies (Func) 3 Antibodies
Immunoassay (IA) 2 Antibodies
Immunofluorescence (IF) 1 Antibodies
Immunohistochemistry (IHC) 36 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 4 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 8 Antibodies
Neutralization (Neut) 2 Antibodies
SDS-PAGE (SDS) 1 Antibodies
Western Blotting (WB) 92 Antibodies

General

Antigen Interleukin 12b (IL12B) Antibodies
Epitope Internal Region
(28), (15), (13), (12), (11), (7), (5), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(126), (58), (27), (19), (6), (4), (2), (2), (1), (1)
Host Goat
(127), (41), (33), (11), (6), (1), (1), (1)
Clonality
Polyclonal
Conjugate This IL12B antibody is un-conjugated
(21), (16), (14), (7), (6), (6)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(153), (140), (38), (23), (14), (9), (6), (5), (4), (4), (4), (3), (2), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-IL12B Antibody

Target Details IL12B Application Details Handling References for anti-IL12B Antibody (ABIN374765) Images
Sequence C-QGKSKREKKD RVFTD
Specificity This antibody recognizes Interleukin-12B (IL12B).
Cross-Reactivity (Details) Species reactivity (tested):Human.
Purification Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.
Immunogen Peptide with sequence from the internal region of the protein sequence according to NP_002178.2.

Target Details IL12B

Product Details anti-IL12B Antibody Application Details Handling References for anti-IL12B Antibody (ABIN374765) Images back to top
Antigen
Alternative Name Interleukin-12 beta / IL12B (IL12B Antibody Abstract)
Background Interleukin 12 (IL12) can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine activated killer cells, and stimulate the production of IFN gamma by resting PBMC. IL12 induces IFN gamma, TNF alpha production in T and NK cells, costimulation of PBL proliferation, proliferation/differentiation of TH1 T lymphocytes. It is downregulated by IL10, homodimeric p40 antagonistic to functional p70 heterodimer. IL12R beta 1 binds p40 but when dimeric with IL12R beta 2 binds p35.Synonyms: CLMF p40, Cytotoxic lymphocyte maturation factor 40 kDa subunit, IL-12 subunit p40, IL-12B, Interleukin-12B, NK cell stimulatory factor chain 2, NKSF2
Gene ID 3593
NCBI Accession NP_002178
UniProt P29460
Pathways JAK-STAT Signaling

Application Details

Product Details anti-IL12B Antibody Target Details IL12B Handling References for anti-IL12B Antibody (ABIN374765) Images back to top
Application Notes Peptide ELISA: Detection Limit: 1/16000. Western Blot: 0.1-0.3 μg/mL. Approx 37 kDa band observed in Human Liver lysates(calculated MW of 37.7 kDa according to NP_002178.2). Immunohistochemistry on Paraffin Embedded Sections: 4-6 μg/mL. In paraffin embedded Human Colon shows cytoplasm staining of individual cells betweencolumns. In paraffin embedded Human Kidney shows cytoplasm staining of epithelial cells of someDCT.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-IL12B Antibody Target Details IL12B Application Details References for anti-IL12B Antibody (ABIN374765) Images back to top
Concentration 0.5 mg/mL
Buffer Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-IL12B Antibody (ABIN374765)

Product Details anti-IL12B Antibody Target Details IL12B Application Details Handling Images back to top
Background publications

Sheth, De, Nolan, Friel, Duffy, Ricciardi, Miller-Graziano, Bankey: "Heat shock protein 27 inhibits apoptosis in human neutrophils." in: The Journal of surgical research, Vol. 99, Issue 1, pp. 129-33, 2001

Images

Product Details anti-IL12B Antibody Target Details IL12B Application Details Handling References for anti-IL12B Antibody (ABIN374765) back to top
Supplier Images
 image for anti-IL12B antibody (Interleukin 12b) (Internal Region) (ABIN374765) anti-Interleukin 12b (IL12B) (Internal Region) antibody
 image for anti-IL12B antibody (Interleukin 12b) (Internal Region) (ABIN374765) anti-Interleukin 12b (IL12B) (Internal Region) antibody (Image 2)