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anti-Sheep (Ovine) IL12B antibody for Flow Cytometry

Recommended IL12B Antibody (supplied by: Log in to see )

Antigen
Interleukin 12b (IL12B) Antibodies
  • CLMF
  • CLMF2
  • IL-12
  • IL-12.p40
  • IL-12B
  • Il-12b
  • IL-12p40
  • Il-12p40
  • Il12
  • IL12B
  • IL12p40
  • Il12p40
  • NKSF
  • NKSF2
  • p40
Reactivity
Cow (Bovine), Human, Sheep (Ovine)
149
59
26
23
20
5
2
2
1
1
Host
Mouse
129
60
36
11
6
1
1
1
Clonality
Monoclonal
Conjugate
This IL12B antibody is un-conjugated
22
17
15
8
6
6
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Functional Studies (Func), Flow Cytometry (FACS)
161
159
41
28
22
17
7
5
4
4
4
2
2
2
1
1
1
Supplier
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Catalog No. ABIN317391
$ 753.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0010482287 ABIN1107794 ELISpot Func EIA FACS WB Mouse IgG2a Log in to see CC301

Similar anti-IL12B Antibodies

Application / Reactivity Sheep (Ovine) Human
ELISA 17 Antibodies 101 Antibodies
ELISpot 1 Antibodies 1 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies 5 Antibodies
Flow Cytometry (FACS) 2 Antibodies 22 Antibodies
Functional Studies (Func) 2 Antibodies 3 Antibodies
Immunohistochemistry (IHC) 1 Antibodies 38 Antibodies
Western Blotting (WB) 18 Antibodies
Dot Blot (DB) 5 Antibodies
ELISA (Capture) 1 Antibodies
Immunoassay (IA) 2 Antibodies
Immunocytochemistry (ICC) 1 Antibodies
Immunofluorescence (IF) 2 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 1 Antibodies

General

Antigen Interleukin 12b (IL12B) Antibodies
Reactivity Cow (Bovine), Human, Sheep (Ovine)
(149), (59), (26), (23), (20), (5), (2), (2), (1), (1)
Host Mouse
(129), (60), (36), (11), (6), (1), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This IL12B antibody is un-conjugated
(22), (17), (15), (8), (6), (6), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Functional Studies (Func), Flow Cytometry (FACS)
(161), (159), (41), (28), (22), (17), (7), (5), (4), (4), (4), (2), (2), (2), (1), (1), (1)
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Product Details anti-IL12B Antibody

Target Details IL12B Application Details Handling Images
Specificity This antibody recognizes Interleukin-12. It has been shown to block the biological activity of IL-12.
Characteristics Synonyms: Interleukin-12B, IL-12B, IL-12 subunit p40, CLMF p40, NKSF2, Cytotoxic lymphocytematuration factor 40 kDa subunit, NK cell stimulatory factor chain 2
Purification Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Immunogen Recombinant bovine IL-12
Clone CC326
Isotype IgG2b

Target Details IL12B

Product Details anti-IL12B Antibody Application Details Handling Images back to top
Antigen
Alternative Name Interleukin-12 beta / IL12B (IL12B Antibody Abstract)
Background Interleukin 12 (IL12) can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine activated killer cells, and stimulate the production of IFN gamma by resting PBMC. IL12 induces IFN gamma, TNF alpha production in T and NK cells, costimulation of PBL proliferation, proliferation/differentiation of TH1 T lymphocytes. It is downregulated by IL10, homodimeric p40 antagonistic to functional p70 heterodimer. IL12R beta 1 binds p40 but when dimeric with IL12R beta 2 binds p35.Synonyms: CLMF p40, Cytotoxic lymphocyte maturation factor 40 kDa subunit, IL-12 subunit p40, IL-12B, Interleukin-12B, NK cell stimulatory factor chain 2, NKSF2
Gene ID 281857
UniProt P46282
Pathways JAK-STAT Signaling

Application Details

Product Details anti-IL12B Antibody Target Details IL12B Handling Images back to top
Application Notes ELISA: 1/100 - 1/1000. Flow cytometry: 1/1000, use 10 μL to label 10^6 cells of 100 l. Functional assays: 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-IL12B Antibody Target Details IL12B Application Details Images back to top
Concentration 1.0 mg/mL
Buffer PBS, pH 7.4, None, None
Preservative Azide free
Storage -20 °C
Storage Comment Store the antibody undiluted at -20 °C. Avoid repeated freezing and thawing.
Shelf life: One year from despatch.
Expiry Date 12 months