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anti-Mouse (Murine) IL2 antibody for Blocking Antibody

Recommended IL2 Antibody (supplied by: Log in to see )

Antigen
Interleukin 2 (IL2) Antibodies
  • Il-2
  • IL-2
  • IL2
  • il2
  • lymphokine
  • POIL2
  • TCGF
Reactivity
Mouse (Murine)
472
257
89
20
18
7
6
4
4
3
2
2
1
1
Host
Rat
297
260
243
17
7
4
Clonality
Monoclonal
Conjugate
This IL2 antibody is un-conjugated
95
51
50
28
21
16
15
12
12
9
7
7
7
7
7
7
7
7
6
6
6
6
5
5
4
4
4
4
4
2
2
2
2
2
1
1
1
1
Application
Blocking Antibody (Inhibition), Immunoprecipitation (IP), ELISA
469
384
219
70
65
55
45
43
38
36
30
27
20
14
13
9
7
6
6
4
4
4
3
2
2
1
1
1
1
1
1
Supplier
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Catalog No. ABIN2689752
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008696448 ABIN1002611 Inhibition ELISA WB Rabbit IgG Log in to see Polyclonal
0.0008696448 ABIN1002615 Inhibition Rat IgG2a Log in to see NYRmIL-2
0.0008696448 ABIN933952 Inhibition ELISA WB Rat IgG2a kappa Log in to see M912297
0.0008696448 ABIN808086 Inhibition IP IHC TCC ELISA Rat Log in to see NYRmIL-2

Top referenced anti-IL2 antibody for Blocking Antibody

Similar anti-IL2 Antibodies

Application / Reactivity Mouse (Murine)
Blocking Antibody (Inhibition) 5 Antibodies
Blocking Peptide (BP) 2 Antibodies
Blocking Reagent (BR) 1 Antibodies
Cellular Assay (CA) 1 Antibodies
Dot Blot (DB) 1 Antibodies
ELISA 129 Antibodies
ELISA (Capture) 2 Antibodies
ELISA (Detection) 1 Antibodies
ELISpot 3 Antibodies
ELISpot (Capture) (ELISPOT (Capture)) 1 Antibodies
ELISpot (Detection) (ELISPOT (Detection)) 1 Antibodies
Enzyme Immunoassay (EIA) 8 Antibodies
FLISA 2 Antibodies
Flow Cytometry (FACS) 96 Antibodies
Functional Studies (Func) 7 Antibodies
Immunoassay (IA) 1 Antibodies
Immunocytochemistry (ICC) 4 Antibodies
Immunofluorescence (IF) 3 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies
Immunohistochemistry (IHC) 16 Antibodies

General

Antigen Interleukin 2 (IL2) Antibodies
Reactivity Mouse (Murine)
(472), (257), (89), (20), (18), (7), (6), (4), (4), (3), (2), (2), (1), (1)
Host Rat
(297), (260), (243), (17), (7), (4)
Clonality (Clone)
Monoclonal   ( )
Conjugate This IL2 antibody is un-conjugated
(95), (51), (50), (28), (21), (16), (15), (12), (12), (9), (7), (7), (7), (7), (7), (7), (7), (7), (6), (6), (6), (6), (5), (5), (4), (4), (4), (4), (4), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Application Blocking Antibody (Inhibition), Immunoprecipitation (IP), ELISA
(469), (384), (219), (70), (65), (55), (45), (43), (38), (36), (30), (27), (20), (14), (13), (9), (7), (6), (6), (4), (4), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-IL2 Antibody

Target Details IL2 Application Details Handling References for anti-IL2 Antibody (ABIN2689752) Images
Brand BD Pharmingen™
Characteristics The JES6-5H4 antibody reacts with mouse interleukin-2 (IL-2) and has been reported to be neutralizing. Expression of IL-2 by stimulated CD4+ and CD4- BALB/c spleen cells. Splenocytes from 6 month old BALB/c mice were stimulated for 5 hours with hamster anti-mouse CD3 (2 μg/mL final concentration, clone 145-2C11, Cat. No. 553057) and hamster anti-mouse CD28 (2 μg/mL final concentration, clone 37.51, Cat. No. 553294) antibodies in the presence of BD GolgiStop™ (3 μM final concentration, Cat. No. 554724). The cells were harvested, stained with 0.06 μg of PE-conjugated rat anti-mouse CD4 (PE-RM4-5, Cat. No. 553049), fixed, permeabilized, and subsequently stained with 0.06 μg of FITC-conjugated rat anti-mouse IL-2 antibody (FITC-JES6-5H4, Cat. No. 554427, left panel). To demonstrate specificity of staining, the binding by the FITC-JES6-5H4 antibody was blocked by preincubation of the conjugated antibody with recombinant mouse IL-2 (0.12 μg, Cat. No. 550069, middle panel), and by preincubation of the fixed/permeabilized cells with unlabeled JES6-5H4 antibody (2.5 μg, Cat. No. 554425, right panel) prior to staining. The quadrant markers for the bivariate dot plots were set based on the autofluorescence controls and verified using the recombinant cytokine blocking and unlabelled antibody blocking specificity controls.

BD Pharmingen™ Purified Rat Anti-Mouse IL-2 - Purified - Clone JES6-5H4 - Isotype Rat IgG2b - Reactivity Ms - 0.1 mg
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Mouse IL-2 Recombinant Protein
Clone JES6
Isotype IgG2b

Target Details IL2

Product Details anti-IL2 Antibody Application Details Handling References for anti-IL2 Antibody (ABIN2689752) Images back to top
Antigen
Alternative Name IL-2 (IL2 Antibody Abstract)
Research Area Cytokines, Immunology, Virology, Inflammation, Cancer
Pathways JAK-STAT Signaling

Application Details

Product Details anti-IL2 Antibody Target Details IL2 Handling References for anti-IL2 Antibody (ABIN2689752) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-IL2 Antibody Target Details IL2 Application Details References for anti-IL2 Antibody (ABIN2689752) Images back to top
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C.

References for anti-IL2 Antibody (ABIN2689752)

Product Details anti-IL2 Antibody Target Details IL2 Application Details Handling Images back to top
Product cited in:

Abrams: "Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies." in: Current protocols in immunology / edited by John E. Coligan ... [et al.], Vol. Chapter 6, pp. Unit 6.20, 2008

Prussin, Metcalfe: "Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies." in: Journal of immunological methods, Vol. 188, Issue 1, pp. 117-28, 1996

Abrams, Roncarolo, Yssel, Andersson, Gleich, Silver: "Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples." in: Immunological reviews, Vol. 127, Issue 9-10, pp. 5-24, 1992