You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Mouse (Murine) IL2 antibody for Neutralization

Recommended IL2 Antibody (supplied by: Log in to see )

Antigen
Interleukin 2 (IL2) Antibodies
  • IL2
  • IL-2
  • il2
  • TCGF
  • lymphokine
  • Il-2
  • POIL2
  • interleukin 2
  • IL2
  • il2
  • Il2
Reactivity
Mouse (Murine)
466
240
98
31
17
6
6
6
4
3
3
3
1
1
Host
Rat
314
259
211
17
5
4
Clonality
Monoclonal
Conjugate
This IL2 antibody is un-conjugated
85
49
49
27
19
17
17
14
12
11
8
8
7
7
7
7
7
7
7
7
6
6
6
4
4
4
4
4
4
4
3
3
2
2
2
1
1
Application
Neutralization (Neut), Western Blotting (WB)
451
385
217
86
71
49
48
48
38
35
26
23
15
10
8
7
7
6
4
4
3
3
2
2
2
1
1
1
1
1
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Catalog No. ABIN1177301
Contact our Customer Service for availability and price in your country.
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN465129 Neut ELISA WB Rat IgG2a Log in to see
1 ABIN465593 Neut ELISA WB Rabbit Log in to see Polyclonal
1 ABIN2689749 FACS IP Neut ELISA WB Rat IgG2a, kappa Log in to see JES6 4
1 ABIN465712 ELISA Neut WB Biotin Rabbit Log in to see Polyclonal
1 ABIN218079 IHC (fro) Neut WB Mouse IgG2a, kappa Log in to see
1 ABIN2288287 Neut ELISA Biotin Rat IgG2b kappa Log in to see 5K300
1 ABIN4324799 BP CyTOF FACS IHC (fro) IP Neut Rat IgG2 Log in to see MAB0844
1 ABIN2288359 Neut ELISA WB PE Rabbit IgG Log in to see Polyclonal
1 ABIN2288464 Neut ELISA WB HRP Rabbit IgG Log in to see Polyclonal
1 ABIN2288462 Neut ELISA WB FITC Rabbit IgG Log in to see Polyclonal
1 ABIN2288457 Neut ELISA WB Alkaline Phosphatase (AP) Rabbit IgG Log in to see Polyclonal
1 ABIN2288460 Neut ELISA WB Biotin Rabbit IgG Log in to see Polyclonal
1 ABIN2288459 Neut ELISA WB APC Rabbit IgG Log in to see Polyclonal
1 ABIN2748973 ICS IP Neut ELISA Rat IgG2a Log in to see JES6-1A12
1 ABIN2748975 FACS IP Neut IHC ELISA WB Rat IgG2b Log in to see JES6-5H4
1 ABIN2748974 FACS ICS IP Neut IHC WB Rat IgG2a Log in to see S4B6-1
1 ABIN2288299 Neut ELISA WB Rabbit IgG Log in to see Polyclonal
1 ABIN2288499 Neut ELISA WB Rabbit IgG Log in to see Polyclonal

Top referenced anti-IL2 antibody for Neutralization

Similar anti-IL2 Antibodies

Application / Reactivity Mouse (Murine)
Blocking Antibody (Inhibition) 5 Antibodies
Blocking Peptide (BP) 2 Antibodies
Blocking Reagent (BR) 1 Antibodies
Cellular Assay (CA) 1 Antibodies
Cytometry by Time of Flight (CyTOF) 2 Antibodies
Dot Blot (DB) 1 Antibodies
ELISA 118 Antibodies
ELISpot 2 Antibodies
Enzyme Immunoassay (EIA) 8 Antibodies
FLISA 2 Antibodies
Flow Cytometry (FACS) 81 Antibodies
Functional Studies (Func) 5 Antibodies
Immunoassay (IA) 1 Antibodies
Immunocytochemistry (ICC) 3 Antibodies
Immunofluorescence (IF) 3 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 1 Antibodies

General

Antigen Interleukin 2 (IL2) Antibodies
Reactivity Mouse (Murine)
(466), (240), (98), (31), (17), (6), (6), (6), (4), (3), (3), (3), (1), (1)
Host Rat
(314), (259), (211), (17), (5), (4)
Clonality (Clone)
Monoclonal   ( )
Conjugate This IL2 antibody is un-conjugated
(85), (49), (49), (27), (19), (17), (17), (14), (12), (11), (8), (8), (7), (7), (7), (7), (7), (7), (7), (7), (6), (6), (6), (4), (4), (4), (4), (4), (4), (4), (3), (3), (2), (2), (2), (1), (1)
Application Neutralization (Neut), Western Blotting (WB)
(451), (385), (217), (86), (71), (49), (48), (48), (38), (35), (26), (23), (15), (10), (8), (7), (7), (6), (4), (4), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 3 references available
Supplier Log in to see

Product Details anti-IL2 Antibody

Target Details IL2 Application Details Handling References for anti-IL2 Antibody (ABIN1177301) Images
Brand BD Pharmingen™
Characteristics Neutralization Activity: This antibody has been reported to be useful for the neutralization of recombinant mouse IL-2. Neutralization activity may be measured with a proliferation assay using 80 pg/mL recombinant mouse IL-2 (Cat. No. 550069) to stimulate CTLL-2 cells at 1x10^5 cells/mL as indicator cells (i.e. preincubation of the antibody with recombinant mouse IL-2 for 60 minutes).
50% Neutralization (ND50) at 5 - 30 ng/mL
>95% Neutralization at 30 - 80 ng/mL
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Sterility 0.2 μm filtered
Endotoxin Level Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.
Immunogen T cell clone supernatant
Clone S4B6
Isotype IgG2a

Target Details IL2

Product Details anti-IL2 Antibody Application Details Handling References for anti-IL2 Antibody (ABIN1177301) Images back to top
Antigen
Alternative Name IL-2 (IL2 Antibody Abstract)
Background The S4B6 antibody reacts with mouse interleukin-2 (IL-2). The immunogen used to generate the S4B6 hyridoma was a T cell clone supernatant. This is a neutralizing antibody.
Research Area Cancer
Pathways JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation

Application Details

Product Details anti-IL2 Antibody Target Details IL2 Handling References for anti-IL2 Antibody (ABIN1177301) Images back to top
Application Notes Western Blot: The S4B6 antibody (purified format) has been reported to be useful for Western blotting. A concentration of 1-5 µg/mL (in conjunction with an AKP-conjugated goat anti-rat Ig secondary antibody) has been reported to enable detection of ≤ 100 ng/lane of recombinant mouse IL-2 (Cat. No. 550069) under reducing conditions.
Restrictions For Research Use only

Handling

Product Details anti-IL2 Antibody Target Details IL2 Application Details References for anti-IL2 Antibody (ABIN1177301) Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer No azide/low endotoxin: Aqueous buffered solution containing protein stabilizer, no preservative, 0.2μm sterile filtered.
Preservative Azide free
Storage 4 °C
Storage Comment Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

References for anti-IL2 Antibody (ABIN1177301)

Product Details anti-IL2 Antibody Target Details IL2 Application Details Handling Images back to top
Product cited in:

Svetić, Jian, Lu, Finkelman, Gause: "Brucella abortus induces a novel cytokine gene expression pattern characterized by elevated IL-10 and IFN-gamma in CD4+ T cells." in: International immunology, Vol. 5, Issue 8, pp. 877-83, 1993

Kubo, Cinader: "Polymorphism of age-related changes in interleukin (IL) production: differential changes of T helper subpopulations, synthesizing IL 2, IL 3 and IL 4." in: European journal of immunology, Vol. 20, Issue 6, pp. 1289-96, 1990

Gillis, Ferm, Ou, Smith: "T cell growth factor: parameters of production and a quantitative microassay for activity." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 120, Issue 6, pp. 2027-32, 1978