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anti-Mouse (Murine) IL2 antibody for Neutralization

Recommended IL2 Antibody (supplied by: Log in to see )

Antigen
Interleukin 2 (IL2) Antibodies
  • Il-2
  • IL-2
  • IL2
  • il2
  • lymphokine
  • POIL2
  • TCGF
Reactivity
Mouse (Murine)
372
218
89
18
13
7
6
4
4
3
2
2
1
1
Host
Rat
256
207
192
17
7
4
Clonality
Monoclonal
Conjugate
This IL2 antibody is un-conjugated
87
45
43
21
21
12
7
6
6
5
4
4
4
4
4
4
3
3
2
2
2
2
1
1
1
1
Application
ELISA (Capture), ELISpot, Immunoprecipitation (IP), Neutralization (Neut)
362
324
153
67
60
43
43
38
33
30
20
17
15
11
8
8
7
6
5
4
4
3
2
2
2
1
1
1
1
1
1
Supplier
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Catalog No. ABIN282505
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008704084 ABIN1177301 Neut WB Rat IgG2a Log in to see S4B6 3
0.0008704084 ABIN465129 Neut ELISA WB Rat IgG2a Log in to see
0.0008704084 ABIN411517 Neut WB Rabbit IgG Log in to see Polyclonal
0.0008704084 ABIN465593 Neut ELISA WB Rabbit Log in to see Polyclonal
0.0008704084 ABIN2689749 FACS IP Neut ELISA WB Rat IgG2a, kappa Log in to see JES6 4
0.0008704084 ABIN2288287 Neut ELISA Biotin Rat IgG2b kappa Log in to see 5K300
0.0008704084 ABIN2288359 Neut ELISA WB PE Rabbit IgG Log in to see Polyclonal
0.0008704084 ABIN2288457 Neut ELISA WB Alkaline Phosphatase (AP) Rabbit IgG Log in to see Polyclonal
0.0008704084 ABIN2288459 Neut ELISA WB APC Rabbit IgG Log in to see Polyclonal
0.0008704084 ABIN2288460 Neut ELISA WB Biotin Rabbit IgG Log in to see Polyclonal
0.0008704084 ABIN2288462 Neut ELISA WB FITC Rabbit IgG Log in to see Polyclonal
0.0008704084 ABIN2288464 Neut ELISA WB HRP Rabbit IgG Log in to see Polyclonal
0.0008704084 ABIN2748973 ICS IP Neut ELISA Rat IgG2a Log in to see JES6-1A12
0.0008704084 ABIN2748974 FACS ICS IP Neut IHC WB Rat IgG2a Log in to see S4B6-1
0.0008704084 ABIN2748975 FACS IP Neut IHC ELISA WB Rat IgG2b Log in to see JES6-5H4
0.0008704084 ABIN2288299 Neut ELISA WB Rabbit IgG Log in to see Polyclonal
0.0008704084 ABIN2288499 Neut ELISA WB Rabbit IgG Log in to see Polyclonal

Top referenced anti-IL2 antibody for Neutralization

Similar anti-IL2 Antibodies

Application / Reactivity Mouse (Murine)
Blocking Antibody (Inhibition) 5 Antibodies
Blocking Peptide (BP) 1 Antibodies
Blocking Reagent (BR) 1 Antibodies
Cellular Assay (CA) 1 Antibodies
Dot Blot (DB) 1 Antibodies
ELISA 93 Antibodies
ELISA (Capture) 2 Antibodies
ELISA (Detection) 1 Antibodies
ELISpot 3 Antibodies
ELISpot (Capture) (ELISPOT (Capture)) 1 Antibodies
ELISpot (Detection) (ELISPOT (Detection)) 1 Antibodies
Enzyme Immunoassay (EIA) 8 Antibodies
FLISA 2 Antibodies
Flow Cytometry (FACS) 76 Antibodies
Functional Studies (Func) 7 Antibodies
Immunoassay (IA) 1 Antibodies
Immunocytochemistry (ICC) 4 Antibodies
Immunofluorescence (IF) 3 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies
Immunohistochemistry (IHC) 15 Antibodies

General

Antigen Interleukin 2 (IL2) Antibodies
Reactivity Mouse (Murine)
(372), (218), (89), (18), (13), (7), (6), (4), (4), (3), (2), (2), (1), (1)
Host Rat
(256), (207), (192), (17), (7), (4)
Clonality (Clone)
Monoclonal   ( )
Conjugate This IL2 antibody is un-conjugated
(87), (45), (43), (21), (21), (12), (7), (6), (6), (5), (4), (4), (4), (4), (4), (4), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1)
Application ELISA (Capture), ELISpot, Immunoprecipitation (IP), Neutralization (Neut)
(362), (324), (153), (67), (60), (43), (43), (38), (33), (30), (20), (17), (15), (11), (8), (8), (7), (6), (5), (4), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-IL2 Antibody

Target Details IL2 Application Details Handling References for anti-IL2 Antibody (ABIN282505) Images
Specificity The JES6-1A12 antibody reacts with mouse interleukin-2 (IL-2). The JES6-1A12 antibody is a neutralizing antibody. Mouse IL-2 (also known as TCGF) is a 17 kDa factor produced mainly by activated CD4+ T cells. IL-2 induces cell cycle progression of resting cells in an antigen non-specific manner and allows clonal expansion of activated T cells. IL-2 also acts on activated B cells, monocytes, NK cells, LAK cells, and on oligodendroglial cells in vitro. In addition, IL-2 plays a role in hematopoiesis, tumor surveillance and anti-inflammatory reactions, and hence is a central regulator of the immune response. Non-glycosylated IL-2 is biologically active.
Characteristics The JES6-1A12 antibody reacts with mouse interleukin-2 (IL-2). The JES6-1A12 antibody is a neutralizing antibody. Mouse IL-2 (also known as TCGF) is a 17 kDa factor produced mainly by activated CD4+ T cells. IL-2 induces cell cycle progression of resting cells in an antigen non- specific manner and allows clonal expansion of activated T cells. IL-2 also acts on activated B cells, monocytes, NK cells, LAK cells, and on oligodendroglial cells in vitro. In addition, IL-2 plays a role in hematopoiesis, tumor surveillance and anti-inflammatory reactions, and hence is a central regulator of the immune response. Non-glycosylated IL-2 is biologically active.
Synonyms: Synonyms: Interleukin-2
Purification Affinity Purified
Endotoxin Level <0.001 ng endotoxin/μg
Grade Functional Grade
Clone JES6-1A12
Isotype IgG2a kappa

Target Details IL2

Product Details anti-IL2 Antibody Application Details Handling References for anti-IL2 Antibody (ABIN282505) Images back to top
Antigen
Alternative Name IL-2 (IL2 Antibody Abstract)
Research Area Cytokines, Immunology, Virology, Inflammation, Cancer
Pathways JAK-STAT Signaling

Application Details

Product Details anti-IL2 Antibody Target Details IL2 Handling References for anti-IL2 Antibody (ABIN282505) Images back to top
Application Notes Applications reported: The JES6-1A12 antibody has been reported for use in ELISA capture, ELISPOT capture, neutralization of IL-2 bioactivity, and IP.

Applications tested: The Functional Grade Purified JES6-1A12 antibody has been tested by LAL assay to verify low endotoxin levels, by ELISA, and in bioassay for neutralization of mouse IL-2 bioactivity. The JES6-1A12 antibody at 0.250 μg/mL has been found to inhibit by 50 % the biological effects of 1.0 ng/mL mouse IL-2 in a CTLL-2 cell proliferation assay. Detailed information and protocols about cytokine bioassays and in vitro cytokine neutralization using antibodies can be found in the BestProtocols® section. The JES6-1A12 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of mouse Interleukin-2 (IL-2) in combination with the biotin JES6-5H4 (13-7021) antibody for detection and recombinant mouse IL-2 (14-8021) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1-4 μg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 500 pg/mL - 4 pg/mL should be included in each ELISA plate.
Restrictions For Research Use only

Handling

Product Details anti-IL2 Antibody Target Details IL2 Application Details References for anti-IL2 Antibody (ABIN282505) Images back to top
Concentration 1 mg/mL
Buffer aqueous buffer, no sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Use in sterile environment.
Storage 4 °C

References for anti-IL2 Antibody (ABIN282505)

Product Details anti-IL2 Antibody Target Details IL2 Application Details Handling Images back to top
Background publications

Abrams, Roncarolo, Yssel, Andersson, Gleich, Silver: "Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples." in: Immunological reviews, Vol. 127, Issue 9-10, pp. 5-24, 1992

Images

Product Details anti-IL2 Antibody Target Details IL2 Application Details Handling References for anti-IL2 Antibody (ABIN282505) back to top
Supplier Images
 image for anti-IL2 antibody (Interleukin 2) (ABIN282505) Splenocytes from mice pre-immunized with PLP139-151 peptide were recall activated 4 w...
 image for anti-IL2 antibody (Interleukin 2) (ABIN282505) anti-Interleukin 2 (IL2) antibody (Image 2)