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Human IL2 ELISA Kit

Recommended IL2 Product (supplied by: Log in to see )

Antigen
Interleukin 2 (IL2) ELISA Kits
  • Il-2
  • IL-2
  • IL2
  • il2
  • lymphokine
  • POIL2
  • TCGF
Epitope
AA 21-153
Reactivity
Human
Alternatives
Kits with alternative reactivity to:
76
71
35
12
11
11
9
9
8
8
5
5
3
2
2
2
1
Methode Type
Sandwich ELISA
Detection Range
15.6-1000 pg/mL
Minimum Detection Limit
15.6 pg/mL
Application
ELISA
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Catalog No. ABIN411295
$ 250.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
0.32693866 ABIN2472116 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant 7.81-500 pg/mL 7.81 pg/mL Log in to see
0.32693866 ABIN414882 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 15.63-1000 pg/mL 15.63 pg/mL Log in to see 3
0.32693866 ABIN924777 Sandwich ELISA Cell Lysate, Serum, Plasma 63-4000 pg/mL 63 pg/mL Log in to see
0.32693866 ABIN1115643 Sandwich ELISA Serum, Plasma, Biological Fluids 31.25-2000 pg/mL 31.25 pg/mL Log in to see
0.32693866 ABIN1888529 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant, Urine 4-1500 pg/mL 4 pg/mL Log in to see
0.32693866 ABIN2708276 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma, Tissue Homogenate 7.8-500 pg/mL 7.8 pg/mL Log in to see
0.32693866 ABIN2708277 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma, Tissue Homogenate 15.6-1000 pg/mL 15.6 pg/mL Log in to see
0.32693866 ABIN2064474 Cell Culture Supernatant, Serum, Plasma Log in to see
0.32693866 ABIN2762272 Cell Culture Supernatant, Serum, Plasma (EDTA), Plasma (citrate), Plasma (heparin) 7.8-500 pg/mL 7.8 pg/mL Log in to see
0.32693866 ABIN2114293 Cell Culture Supernatant, Serum, Plasma (EDTA - heparin - citrate) Log in to see
0.0012117105 ABIN365161 Sandwich ELISA Serum, Plasma, Tissue Homogenate 3.12-200 pg/mL 3.12 pg/mL Log in to see 1
0.0012117105 ABIN1873505 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 1.56-100 pg/mL 1.56 pg/mL Log in to see
0.0012117105 ABIN3032945 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 2.1-1500 pg/mL 2.1 pg/mL Log in to see
0.0012117105 ABIN1446048 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma, Biological Samples Log in to see 1
0.0012117105 ABIN509578 Sandwich ELISA Serum, Cell Culture Supernatant, Tissue Homogenate, Plasma, Body Fluids 50-1000 pg/mL 50 pg/mL Log in to see
0.0012117105 ABIN625022 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant, Urine Log in to see 7
0.0012117105 ABIN455560 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma 31.2-2000 pg/mL 31.2 pg/mL Log in to see
0.0012117105 ABIN1028987 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 15.625-1000 pg/mL 15.625 pg/mL Log in to see
0.0012117105 ABIN1112643 Sandwich ELISA Serum, Plasma, Body Fluids, Tissue Lysate, Cell Culture Supernatant 15.6-1000 pg/mL 15.6 pg/mL Log in to see
0.0012117105 ABIN2703209 Sandwich ELISA Tissue Lysate, Cell Lysate 4-1500 pg/mL 4 pg/mL Log in to see

Top referenced IL2 ELISA Kit for ELISA

  • Human IL2 ELISA Kit for Sandwich ELISA - ABIN411295 (21 Publications): Chen, Ni, Ye, Sun, Li, Xu: Stephanthraniline A inhibits the proliferation and activation of T cells in vitro and in vivo. in European journal of pharmacology 2012 (PubMed)

  • Human IL2 ELISA Kit for Sandwich ELISA - ABIN625022 (7 Publications): Schoof, Terashima, Batter, Douville, Richie, Eberlein: Survival characteristics of metastatic renal cell carcinoma patients treated with lymphokine-activated killer cells plus interleukin-2. in Urology 1993 (PubMed)

  • Human IL2 ELISA Kit for Sandwich ELISA - ABIN2690564 (5 Publications): Stern, Pan, Urdal, Mochizuki, DeChiara, Blacher, Wideman, Gillis: Purification to homogeneity and partial characterization of interleukin 2 from a human T-cell leukemia. in Proceedings of the National Academy of Sciences of the United States of America 1984 (PubMed)

  • Human IL2 ELISA Kit for Sandwich ELISA - ABIN414882 (3 Publications): Alders, Costa-Font, de Klerk, Frank: What is the impact of policy differences on nursing home utilization? The cases of Germany and the Netherlands. in Health policy (Amsterdam, Netherlands) 2015 (PubMed)

  • Human IL2 ELISA Kit for Sandwich ELISA - ABIN1446048 (1 Publications): Bellanger, Pallandre, Gbaguidi-Haore, Knapp, Malézieux, Lignon, Borg, Millon: Investigating the impact of Echinococcus multilocularis vesicular fluid on human cells from healthy blood donors. in Journal of immunological methods 2015 (PubMed)

  • Human IL2 ELISA Kit for Sandwich ELISA - ABIN365161 (1 Publications): Assareh, Haybar, Yoosefi, Bozorgmanesh: Bedside-Friendly Prediction for Presence of Post-Myocardial lnfarction Systolic Dysfunction Using Multimarker Panel: Integrating Salivary Diagnostics into Clinical Practice. in Korean circulation journal 2013 (PubMed)

Similar IL2 ELISA Kits

Application / Reactivity Human
ELISA 55 ELISA Kits

General

Antigen Interleukin 2 (IL2) ELISA Kits
Epitope AA 21-153
Reactivity Human
Kits with alternative reactivity to:
(76), (71), (35), (12), (11), (11), (9), (9), (8), (8), (5), (5), (3), (2), (2), (2), (1)
Methode Type Sandwich ELISA
Detection Range 15.6-1000 pg/mL
Minimum Detection Limit 15.6 pg/mL
Application ELISA
Pubmed 20 references available
Supplier Log in to see

Product Details IL2 ELISA Kit

Target details Application Details Handling References for IL2 Kit (ABIN411295) Images
Purpose For quantitative detection of human IL-2 in cell culture supernates, serum and plasma( heparin, EDTA, citrate).
Brand PicoKine™
Sample Type Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate)
Detection Method Colorimetric
Specificity Natural and recombinant human IL-2
Cross-Reactivity (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivity <1pg/mL
Components
  • 96-well plate precoated with anti- human IL-2 antibod - 1
  • Lyophilized recombinant human IL-2 standar - 10ng/tubex2
  • Biotinylated anti- human IL-2 antibod - 130ul(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC - 130ul(dilution 1:100)
  • Sample diluent buffe - 30ml
  • Antibody diluent buffe - 12ml
  • ABC diluent buffe - 12ml
  • TMB color developing agen - 10ml
  • TMB stop solutio - 10ml
Material not included Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: E.coli
Immunogen sequence: A21-T153

Target details

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Antigen
Alternative Name IL2 (IL2 ELISA Kit Abstract)
Background Interleukin-2(IL2), formerly referred to as T-cell growth factor, is a powerfully immunoregulatory lymphokine that is produced by lectin- or antigen-activated T cells. It is produced not only by mature T lymphocytes on stimulation but also constitutively by certain T-cell lymphoma cell lines. The lymphokine interleukin-2(IL-2) is responsible for autocrine cell cycle progression and regulation of immune responses. IL-2 expression in mature thymocytes and T cells has been found to be tightly controlled by monoallelic expression. IL-2 can act as a growth hormone for both B and T lymphocytes. The human gene for interleukin 2(IL2) is assigned to chromosome 4. Human IL-2 is a 15.5KDa glycoprotein, consisting of 153 amino acids in precursor form and 133 amino acids in mature form.
Gene ID 3558
UniProt P60568
Research Area Immunology, Innate Immunity, Cytokines
Pathways JAK-STAT Signaling

Application Details

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Application Notes Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Plate Pre-coated,Strips (12 x 8)
Protocol human IL-2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-2 has been precoated onto 96-well plates. Standards (E.coli,A21-T153) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL-2 amount of sample captured in plate.
Assay Procedure Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human IL-2 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma( heparin, EDTA, citrate) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human IL-2 standard solution and each sample be measured in duplicate.
Assay Precision
  • Sample 1: n=16, Mean(pg/ml): 75, Standard deviation: 3.45, CV(%): 4.6
  • Sample 2: n=16, Mean(pg/ml): 272, Standard deviation: 9.52, CV(%): 3.5
  • Sample 3: n=16, Mean(pg/ml): 537, Standard deviation: 16.65, CV(%): 3.1,
  • Sample 1: n=24, Mean(pg/ml): 96, Standard deviation: 6.048, CV(%): 6.3
  • Sample 2: n=24, Mean(pg/ml): 310, Standard deviation: 16.12, CV(%): 5.2
  • Sample 3: n=24, Mean(pg/ml): 643, Standard deviation: 39.2, CV(%): 6.1
Restrictions For Research Use only

Handling

Product Details IL2 ELISA Kit Target details Application Details References for IL2 Kit (ABIN411295) Images back to top
Storage -20 °C,4 °C
Storage Comment Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date 12 months

References for IL2 Kit (ABIN411295)

Product Details IL2 ELISA Kit Target details Application Details Handling Images back to top
Product cited in:

Tong, Zhang, Zhao, Liu, Han: "Traffic-related PM2.5 induces cytosolic [Ca²⁺] increase regulated by Orai1, alters the CaN-NFAT signaling pathway, and affects IL-2 and TNF-α cytoplasmic levels in Jurkat T-cells." in: Archives of environmental contamination and toxicology, Vol. 68, Issue 1, pp. 31-7, 2015

Hu, Jing, Wang, Zhang, Yong, Wang: "The positive effects of Ginsenoside Rg1 upon the hematopoietic microenvironment in a D-Galactose-induced aged rat model." in: BMC complementary and alternative medicine, Vol. 15, pp. 119, 2015

Cheng, Wu, Gao, Xu: "Analysis of influence of baicalin joint resveratrol retention enema on the TNF-α, SIgA, IL-2, IFN-γ of rats with respiratory syncytial virus infection." in: Cell biochemistry and biophysics, Vol. 70, Issue 2, pp. 1305-9, 2014

Yu, Liu, Chen, Zeng: "Anti-inflammatory effect of Qingwen Baidu Decoction (清瘟败毒饮) in sepsis rats." in: Chinese journal of integrative medicine, Vol. 20, Issue 12, pp. 934-43, 2014

Eker, Yavasci, Cangur, Kirli, Sarandol: "Can BDNF and IL-2 be indicators for the diagnosis in schizophrenic patients with depressive symptoms?" in: Acta neuropsychiatrica, Vol. 26, Issue 5, pp. 291-7, 2014

Liang, Liu, Chen, Sun: "Antitumor activity of the protein and small molecule component fractions from Agrocybe aegerita through enhancement of cytokine production." in: Journal of medicinal food, Vol. 17, Issue 4, pp. 439-46, 2014

Li, He, Wang, Shu, Liu: "USP2a positively regulates TCR-induced NF-κB activation by bridging MALT1-TRAF6." in: Protein & cell, Vol. 4, Issue 1, pp. 62-70, 2013

Sofian, Aghakhani, Farazi, Banifazl, Eslamifar, Rashidi, Khadem Sadegh, Ramezani: "Serum profile of T helper 1 and T helper 2 cytokines in hepatitis C virus infected patients." in: Hepatitis monthly, Vol. 12, Issue 12, pp. e6156, 2013

Liu, Shan, Dong, Liu, Ma, Liu: "Combined early fluid resuscitation and hydrogen inhalation attenuates lung and intestine injury." in: World journal of gastroenterology, Vol. 19, Issue 4, pp. 492-502, 2013

Zhong, Cheng, Long, He, Qi, Xiang, Zhao, Zhu: "Dynamically expressed microRNA-15b modulates the activities of CD8+ T lymphocytes in mice with Lewis lung carcinoma." in: Journal of translational medicine, Vol. 11, pp. 71, 2013

Zhu, Qiu, Liu, Dong, Weng, Hu, Kuang, Gao, Dan, Tao: "Tolerogenic dendritic cells generated by RelB silencing using shRNA prevent acute rejection." in: Cellular immunology, Vol. 274, Issue 1-2, pp. 12-8, 2012

Guida, Luongo, Aviello, Palazzo, De Chiaro, Gatta, Boccella, Marabese, Zjawiony, Capasso, Izzo, de Novellis, Maione: "Salvinorin A reduces mechanical allodynia and spinal neuronal hyperexcitability induced by peripheral formalin injection." in: Molecular pain, Vol. 8, pp. 60, 2012

Liu, Shen, Wei, Mao, Huang: "Immunomodulatory activity of butanol extract from Solanum lyratum in tumor-bearing mice." in: Immunopharmacology and immunotoxicology, Vol. 33, Issue 1, pp. 100-6, 2011

Xu, Feng, Wang, Zhu, Lin, Lou, Xiang, He, Zheng, Tang, Zuo: "Phytoestrogen calycosin-7-O-β-D-glucopyranoside ameliorates advanced glycation end products-induced HUVEC damage." in: Journal of cellular biochemistry, Vol. 112, Issue 10, pp. 2953-65, 2011

Chen, Ni, Ye, Sun, Li, Xu: "Comparison of immunosuppressive activity of stephanoside E and its aglycone from Stephanotis mucronata in vitro." in: International immunopharmacology, Vol. 10, Issue 10, pp. 1153-60, 2010

Li, Liang, Ma, Hu, Yan, Li, Fang, Wang, Zhang, Xie, Zhang: "Calcineurin mediates the immune response of hemocytes through NF-kappaB signaling pathway in pearl oyster (Pinctada fucata)." in: Fish & shellfish immunology, Vol. 28, Issue 2, pp. 253-60, 2010

Background publications

Chen, Ni, Ye, Sun, Li, Xu: "Stephanthraniline A inhibits the proliferation and activation of T cells in vitro and in vivo." in: European journal of pharmacology, Vol. 685, Issue 1-3, pp. 186-97, 2012

Qiu, Zhu, Liu, Dong, Weng, Hu, Kuang, Gao, Dan, Tao: "Lentiviral-mediated shRNA against RelB induces the generation of tolerogenic dendritic cells." in: International immunopharmacology, Vol. 12, Issue 3, pp. 501-9, 2012

Cen, Hu, Zhou, Yang, Chen, Schmidt, Li: "Enhancement of specific cellular immune response induced by DNA vaccines encoding PML-RARalpha and hIL-2 genes." in: Hematology (Amsterdam, Netherlands), Vol. 15, Issue 2, pp. 88-95, 2010

Lowenthal, Zubler, Nabholz, MacDonald: "Similarities between interleukin-2 receptor number and affinity on activated B and T lymphocytes." in: Nature, Vol. 315, Issue 6021, pp. 669-72, 1985

Images

Product Details IL2 ELISA Kit Target details Application Details Handling References for IL2 Kit (ABIN411295) back to top
Supplier Images
ELISA image for IL2 ELISA Kit (Interleukin 2) (ABIN411295) Human IL-2 PicoKine ELISA Kit standard curve