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anti-Human IL3RA antibody for Immunohistochemistry

Recommended IL3RA Antibody (supplied by: Log in to see )

Antigen
Interleukin 3 Receptor, alpha (Low Affinity) (IL3RA) Antibodies
  • Cyrl
  • CD123
  • IL3R
  • IL3RAY
  • IL3RX
  • IL3RY
  • hIL-3Ra
  • CDw123
  • SUT-1
  • interleukin 3 receptor, alpha
  • interleukin 3 receptor, alpha (low affinity)
  • interleukin 3 receptor, alpha chain
  • Il3ra
  • IL3RA
Epitope
AA 20-305
17
13
13
11
11
10
8
8
5
5
5
5
5
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Human
243
52
3
Host
Mouse
152
99
37
2
Clonality
Monoclonal
Conjugate
This IL3RA antibody is un-conjugated
25
22
19
11
10
7
5
5
5
4
4
4
4
3
3
3
3
3
3
3
3
2
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Neutralization (Neut), Western Blotting (WB)
172
143
57
41
31
29
28
28
16
11
10
8
5
5
3
3
1
1
1
1
1
1
1
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Catalog No. ABIN4899495
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
8.229858 ABIN4899494 FACS ICC IHC Neut WB Mouse IgG1 AA 20-305 Log in to see 32703 2
8.229858 ABIN2490718 IHC ELISA WB Rabbit IgG Tyr593 Log in to see Polyclonal
8.229858 ABIN4325125 ELISA FACS ICC IF IHC IHC (p) WB Mouse IgG1 Log in to see 8E11C5
8.229858 ABIN1845738 ICC FACS IF IHC ELISA WB Mouse IgG1 AA 200-305 Log in to see
1 ABIN1724855 ICC FACS IHC ELISA WB Mouse IgG1 AA 200-305 Log in to see 8E11C5 2
1 ABIN269380 FACS IHC (fro) IHC WB Mouse IgG1 kappa Log in to see 6H6 2
1 ABIN4325126 IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal 1
1 ABIN4880790 ELISA FACS ICC IHC WB Mouse IgG1 AA 200-305 Log in to see 8E11C5
1 ABIN4325132 IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal
1 ABIN4325135 FACS IHC IHC (fro) WB DyLight 405 Mouse IgG1 kappa Log in to see 6H6
1 ABIN4325142 FACS IHC IHC (fro) WB DyLight 488 Mouse IgG1 kappa Log in to see 6H6
1 ABIN2291917 IHC ELISA Rabbit IgG pTyr593 Log in to see Polyclonal
1 ABIN2291928 IHC ELISA HRP Rabbit IgG pTyr593 Log in to see Polyclonal
1 ABIN2291930 IHC ELISA PE Rabbit IgG pTyr593 Log in to see Polyclonal
1 ABIN2291921 IHC ELISA APC Rabbit IgG pTyr593 Log in to see Polyclonal
1 ABIN2291926 IHC ELISA FITC Rabbit IgG pTyr593 Log in to see Polyclonal
1 ABIN2288935 IC FACS IHC ELISA WB Mouse IgG1 AA 200-305 Log in to see
1 ABIN4325131 BP IHC IHC (fro) Neut WB Mouse IgG1 Log in to see MM0403-11A23
1 ABIN4225847 FACS IHC IHC (fro) WB Alexa Fluor 405 Mouse IgG1 kappa Log in to see 6H6
1 ABIN4225848 FACS IHC IHC (fro) WB Alexa Fluor 488 Mouse IgG1 kappa Log in to see 6H6

Top referenced anti-IL3RA antibody for Immunohistochemistry

Similar anti-IL3RA Antibodies

Application / Reactivity Human
Blocking Peptide (BP) 1 Antibodies
Blocking Reagent (BR) 3 Antibodies
Dot Blot (DB) 10 Antibodies
ELISA 52 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies
Flow Cytometry (FACS) 138 Antibodies
Fluorescence Microscopy (FM) 1 Antibodies
Functional Studies (Func) 1 Antibodies
Immunochromatography (IC) 3 Antibodies
Immunocytochemistry (ICC) 18 Antibodies
Immunofluorescence (IF) 16 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 42 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 1 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 5 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 26 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 14 Antibodies
Immunoprecipitation (IP) 28 Antibodies

General

Antigen Interleukin 3 Receptor, alpha (Low Affinity) (IL3RA) Antibodies
Epitope AA 20-305
(17), (13), (13), (11), (11), (10), (8), (8), (5), (5), (5), (5), (5), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(243), (52), (3)
Host Mouse
(152), (99), (37), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This IL3RA antibody is un-conjugated
(25), (22), (19), (11), (10), (7), (5), (5), (5), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Neutralization (Neut), Western Blotting (WB)
(172), (143), (57), (41), (31), (29), (28), (28), (16), (11), (10), (8), (5), (5), (3), (3), (1), (1), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-IL3RA Antibody

Target Details IL3RA Application Details Handling References for anti-IL3RA Antibody (ABIN4899495) Images
Specificity Detects human IL-3 R alpha/CD123 in direct ELISAs and Western blots.
Purification Protein A or G purified from hybridoma culture supernatant
Sterility 0.2 μm filtered
Endotoxin Level <0.10 EU per 1 g of the antibody by the LAL method.
Immunogen S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL-3 R alpha/CD123, Lys20-Arg305, predicted, Accession # P26951
Clone 32703
Isotype IgG1

Target Details IL3RA

Product Details anti-IL3RA Antibody Application Details Handling References for anti-IL3RA Antibody (ABIN4899495) Images back to top
Antigen
Alternative Name IL-3 R alpha/cd123 (IL3RA Antibody Abstract)
Gene ID 3563
UniProt P26951
Pathways JAK-STAT Signaling

Application Details

Product Details anti-IL3RA Antibody Target Details IL3RA Handling References for anti-IL3RA Antibody (ABIN4899495) Images back to top
Application Notes Western Blot 1 μg/mL, Flow Cytometry 2.5 μg/10^6 cells, Immunohistochemistry 8-25 μg/mL, Neutralization 0.6-1.2 μg/mL, Immunocytochemistry 8-25 μg/mL, CyTOF-ready
Restrictions For Research Use only

Handling

Product Details anti-IL3RA Antibody Target Details IL3RA Application Details References for anti-IL3RA Antibody (ABIN4899495) Images back to top
Reconstitution Reconstitute at 0.5 mg/mL in sterile PBS.
Buffer 0.2 m filtered solution in PBS with Trehalose.
Preservative Without preservative
Storage 4 °C/-20 °C/-80 °C
Storage Comment Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 C as supplied. 1 month, 2 to 8 C under sterile conditions after reconstitution. 6 months, -20 to -70 C under sterile conditions after reconstitution.
Expiry Date 12 months

References for anti-IL3RA Antibody (ABIN4899495)

Product Details anti-IL3RA Antibody Target Details IL3RA Application Details Handling Images back to top
Product cited in:

Leskela, Serrano, de la Fuente, Rodríguez-Muñoz, Ramos-Levi, Sampedro-Nuñez, Sánchez-Madrid, González-Amaro, Marazuela: "Graves' disease is associated with a defective expression of the immune regulatory molecule galectin-9 in antigen-presenting dendritic cells." in: PLoS ONE, Vol. 10, Issue 4, pp. e0123938, 2015 Method employed by authors: Immunohistochemistry (Frozen Sections) (IHC (fro)) (Sample species: Human).

Bovolenta, Pilotti, Mauri, Turci, Ciancianaini, Fisicaro, Bertazzoni, Poli, Casoli et al.: "Human T-cell leukemia virus type 2 induces survival and proliferation of CD34(+) TF-1 cells through activation of STAT1 and STAT5 by secretion of interferon-gamma and granulocyte ..." in: Blood, Vol. 99, Issue 1, pp. 224-31, 2001 Method employed by authors: Neutralization (Neut) (Sample species: Human).

Images

Product Details anti-IL3RA Antibody Target Details IL3RA Application Details Handling References for anti-IL3RA Antibody (ABIN4899495) back to top
Supplier Images
Neutralization (Neut) image for anti-IL3RA antibody (Interleukin 3 Receptor, alpha (Low Affinity)) (AA 20-305) (ABIN4899495) Cell Proliferation Induced by IL-3 and Neutralization by Human IL-3 R alpha/CD123 Ant...
Immunocytochemistry (ICC) image for anti-IL3RA antibody (Interleukin 3 Receptor, alpha (Low Affinity)) (AA 20-305) (ABIN4899495) IL-3 R alpha/CD123 in Human Peripheral Blood Lymphocytes. IL-3 R alpha/CD123 was dete...
Immunohistochemistry (IHC) image for anti-IL3RA antibody (Interleukin 3 Receptor, alpha (Low Affinity)) (AA 20-305) (ABIN4899495) IL-3 R alpha/CD123 in Human Tonsil. IL-3 R alpha/CD123 was detected in immersion fixe...