anti-Human IL3RA antibody for Immunohistochemistry (Formalin-fixed Sections)

Recommended IL3RA Antibody (supplied by: Log in to see )

Antigen
Interleukin 3 Receptor, alpha (Low Affinity) (IL3RA) Antibodies
  • Cyrl
  • CD123
  • IL3R
  • IL3RAY
  • IL3RX
  • IL3RY
  • hIL-3Ra
  • CDw123
  • SUT-1
  • interleukin 3 receptor, alpha
  • interleukin 3 receptor, alpha (low affinity)
  • interleukin 3 receptor, alpha chain
  • Il3ra
  • IL3RA
Reactivity
Human
284
55
4
Host
Mouse
192
99
40
3
Clonality
Monoclonal
Conjugate
This IL3RA antibody is un-conjugated
26
25
22
13
12
10
9
7
7
6
5
5
5
5
5
5
5
5
5
4
4
3
3
3
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunoprecipitation (IP), Western Blotting (WB)
198
177
77
75
41
40
38
30
29
13
9
8
8
6
4
4
3
3
2
1
1
1
1
1
1
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Catalog No. ABIN1302506
$ 231.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1981851 IHC (f) FACS IP WB Biotin Mouse IgG1 Log in to see 6H6 3
1 ABIN1558083 FACS IHC (f) IP WB APC Mouse IgG1 Log in to see 6H6 3
1 ABIN1176839 FACS IHC (f) IP WB PE Mouse IgG1 Log in to see 6H6 3
1 ABIN2749104 IHC (f) FACS IP WB FITC Mouse IgG1 Log in to see 6H6 3

General

Antigen Interleukin 3 Receptor, alpha (Low Affinity) (IL3RA) Antibodies
Reactivity Human
(284), (55), (4)
Host Mouse
(192), (99), (40), (3)
Clonality (Clone)
Monoclonal   ( )
Conjugate This IL3RA antibody is un-conjugated
(26), (25), (22), (13), (12), (10), (9), (7), (7), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (4), (4), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunoprecipitation (IP), Western Blotting (WB)
(198), (177), (77), (75), (41), (40), (38), (30), (29), (13), (9), (8), (8), (6), (4), (4), (3), (3), (2), (1), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-IL3RA Antibody

Target Details IL3RA Application Details Handling ProductDetails: References for anti-IL3RA antibody (ABIN1302506) Images
Specificity The mouse monoclonal antibody 6H6 recognizes CD123 (interleukin 3 receptor alpha), a 60-70 kDa transmembrane protein expressed by myeloid precursors, megakaryocytes, macrophages, dendritic cells, mast cells, basophils, and some B cells. This antibody does not inhibit IL-3 binding to its receptor.
Purification Purified from cell culture supernatant by protein-A affinity chromatography.
Purity > 95 % (by SDS-PAGE)
Immunogen IL3 receptor alpha chain expressed on the surface of transiently transfected COS cells
Clone 6H6
Isotype IgG1

Target Details IL3RA

Product Details anti-IL3RA Antibody Application Details Handling ProductDetails: References for anti-IL3RA antibody (ABIN1302506) Images back to top
Antigen
Alternative Name IL3RA (IL3RA Antibody Abstract)
Background CD123 is the alpha chain of interleukin 3 receptor (IL-3R alpha). This subunit heterodimerizes with the interleukin 3 receptor beta chain (CD131), which is shared with other receptors. CD123 interacts with IL-3 specifically, but with low affinity, and association with the beta subunit confers high affinity binding to the receptor heterodimer. Both chains are required for signaling, but receptor activation and signal transduction depend on IL-3 binding to CD123 as the initial step.
Gene ID 3563
Research Area Surface Receptors of Immune Cells, CD Antigens
Pathways JAK-STAT Signaling

Application Details

Product Details anti-IL3RA Antibody Target Details IL3RA Handling ProductDetails: References for anti-IL3RA antibody (ABIN1302506) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-IL3RA Antibody Target Details IL3RA Application Details ProductDetails: References for anti-IL3RA antibody (ABIN1302506) Images back to top
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.

ProductDetails: References for anti-IL3RA antibody (ABIN1302506)

Product Details anti-IL3RA Antibody Target Details IL3RA Application Details Handling Images back to top
Product cited in:

Martín-Gayo, Sierra-Filardi, Corbí, Toribio: "Plasmacytoid dendritic cells resident in human thymus drive natural Treg cell development." in: Blood, Vol. 115, Issue 26, pp. 5366-75, 2010

Herling, Teitell, Shen, Medeiros, Jones: "TCL1 expression in plasmacytoid dendritic cells (DC2s) and the related CD4+ CD56+ blastic tumors of skin." in: Blood, Vol. 101, Issue 12, pp. 5007-9, 2003