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anti-Guinea Pig IL5 antibody for Western Blotting

Recommended IL5 Antibody (supplied by: Log in to see )

Antigen
Interleukin 5 (IL5) Antibodies
  • EDF
  • IL-5
  • Il-5
  • IL5
  • Pro-TRH
  • TRF
Reactivity
Guinea Pig, Human, Mouse (Murine)
138
108
19
3
2
2
1
1
1
1
Host
Rat
106
94
12
2
Clonality
Monoclonal
Conjugate
This IL5 antibody is un-conjugated
42
11
4
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Western Blotting (WB)
110
105
63
34
16
14
11
11
10
9
9
8
8
6
5
4
4
4
4
1
1
Supplier
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Catalog No. ABIN118519
$ 731.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0011773661 ABIN2475029 FACS ELISA WB Rat IgG1 Log in to see TRFK5 4
0.0011773661 ABIN1868679 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG Log in to see Polyclonal

Top referenced anti-IL5 antibody for Western Blotting

Similar anti-IL5 Antibodies

Application / Reactivity Mouse (Murine)
Blocking Antibody (Inhibition) 2 Antibodies
ELISA 46 Antibodies
ELISA (Capture) 2 Antibodies
ELISA (Detection) 1 Antibodies
ELISpot 3 Antibodies
ELISpot (Capture) (ELISPOT (Capture)) 1 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies
Flow Cytometry (FACS) 44 Antibodies
Functional Studies (Func) 4 Antibodies
Immunocytochemistry (ICC) 1 Antibodies
Immunofluorescence (IF) 6 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 5 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 5 Antibodies
Immunoprecipitation (IP) 2 Antibodies
Intracellular Staining (ICS) 6 Antibodies

General

Antigen Interleukin 5 (IL5) Antibodies
Reactivity Guinea Pig, Human, Mouse (Murine)
(138), (108), (19), (3), (2), (2), (1), (1), (1), (1)
Host Rat
(106), (94), (12), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This IL5 antibody is un-conjugated
(42), (11), (4), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Western Blotting (WB)
(110), (105), (63), (34), (16), (14), (11), (11), (10), (9), (9), (8), (8), (6), (5), (4), (4), (4), (4), (1), (1)
Pubmed 4 references available
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Product Details anti-IL5 Antibody

Target Details IL5 Application Details Handling References for anti-IL5 Antibody (ABIN118519) Images
Specificity This antibody is specific for Interleukin 5 (IL-5). Clone TRFK5 may be used to neutralise IL-5 bioactivity in a functional assay (1,2).
Purification Affinity chromatography on Protein G
Immunogen Purified natural murine IL-5.
Clone TRFK5
Isotype IgG1

Target Details IL5

Product Details anti-IL5 Antibody Application Details Handling References for anti-IL5 Antibody (ABIN118519) Images back to top
Antigen
Alternative Name Interleukin-5 / IL5 (IL5 Antibody Abstract)
Background IL-5 is a T cell derived haematopoietic growth factor, which stimulates eosinophil differentiation and activation.Synonyms: B-cell differentiation factor I, Eosinophil differentiation factor, IL-5, T-cell replacing factor, TRF
Gene ID 3567
NCBI Accession NP_000870
UniProt P05113
Pathways JAK-STAT Signaling

Application Details

Product Details anti-IL5 Antibody Target Details IL5 Handling References for anti-IL5 Antibody (ABIN118519) Images back to top
Application Notes Flow Cytometry: Membrane permeabilisation is required for this application. ELISA: This antibody may be used in combination with clone TRFK4 (ABIN181863) in ELISA and ELISPOT assays. Western Blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-IL5 Antibody Target Details IL5 Application Details References for anti-IL5 Antibody (ABIN118519) Images back to top
Concentration 1.0 mg/mL
Buffer PBS containing 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-IL5 Antibody (ABIN118519)

Product Details anti-IL5 Antibody Target Details IL5 Application Details Handling Images back to top
Background publications

Akdis, Akdis, Blesken, Wymann, Alkan, Mueller, Blaser: "Epitope-specific T cell tolerance to phospholipase A2 in bee venom immunotherapy and recovery by IL-2 and IL-15 in vitro." in: The Journal of clinical investigation, Vol. 98, Issue 7, pp. 1676-83, 1996

Abrams, Roncarolo, Yssel, Andersson, Gleich, Silver: "Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples." in: Immunological reviews, Vol. 127, Issue 9-10, pp. 5-24, 1992

Betz, Fox: "Regulation and development of cytochrome c-specific IL-4-producing T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 145, Issue 4, pp. 1046-52, 1990

Schumacher, OGarra, Shrader, van Kimmenade, Bond, Mosmann, Coffman: "The characterization of four monoclonal antibodies specific for mouse IL-5 and development of mouse and human IL-5 enzyme-linked immunosorbent." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 141, Issue 5, pp. 1576-81, 1988