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anti-Human IL5 antibody for ELISpot

Recommended IL5 Antibody (supplied by: Log in to see )

Antigen
Interleukin 5 (IL5) Antibodies
  • IL5
  • Il-5
  • IL-5
  • TRF
  • EDF
  • Pro-TRH
  • interleukin 5
  • Interleukin 5
  • interleukin 5 (colony-stimulating factor, eosinophil)
  • interleukin-5-like
  • thyrotropin-releasing hormone
  • IL5
  • Il5
  • IL-5
  • LOC100358075
  • TRH
Reactivity
Human
168
129
22
16
16
2
2
1
1
1
Host
Rat
122
97
22
2
Clonality
Monoclonal
Conjugate
This IL5 antibody is un-conjugated
40
14
5
5
4
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Application
ELISpot, Neutralization (Neut), ELISA
119
114
94
21
19
13
11
9
9
8
8
7
6
6
5
4
2
2
1
1
Options
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Catalog No. ABIN1177282
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN2748992 ELISpot ICS Neut ELISA WB Mouse IgG1 Log in to see QS-5
1 ABIN1177353 ELISpot ELISA Log in to see
1 ABIN1448856 ELISpot Log in to see

Top referenced anti-IL5 antibody for ELISpot

Similar anti-IL5 Antibodies

Application / Reactivity Human
Blocking Antibody (Inhibition) 6 Antibodies
ELISA 77 Antibodies
ELISA (Capture) 4 Antibodies
ELISA (Detection) 2 Antibodies
ELISpot 4 Antibodies
Enzyme Immunoassay (EIA) 7 Antibodies
Flow Cytometry (FACS) 63 Antibodies
Functional Studies (Func) 9 Antibodies
Immunocytochemistry (ICC) 2 Antibodies
Immunofluorescence (IF) 6 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 13 Antibodies
Immunohistochemistry (IHC) 15 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 7 Antibodies
Immunoprecipitation (IP) 5 Antibodies

General

Antigen Interleukin 5 (IL5) Antibodies
Reactivity Human
(168), (129), (22), (16), (16), (2), (2), (1), (1), (1)
Host Rat
(122), (97), (22), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This IL5 antibody is un-conjugated
(40), (14), (5), (5), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application ELISpot, Neutralization (Neut), ELISA
(119), (114), (94), (21), (19), (13), (11), (9), (9), (8), (8), (7), (6), (6), (5), (4), (2), (2), (1), (1)
Pubmed 2 references available
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Product Details anti-IL5 Antibody

Target Details IL5 Application Details Handling References for anti-IL5 Antibody (ABIN1177282) Images
Brand BD Pharmingen™
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Sterility 0.2 μm filtered
Endotoxin Level Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.
Immunogen Recombinant Human IL-5
Clone JES1-5A10
Isotype IgG2a

Target Details IL5

Product Details anti-IL5 Antibody Application Details Handling References for anti-IL5 Antibody (ABIN1177282) Images back to top
Antigen
Alternative Name IL-5 (IL5 Antibody Abstract)
Background The JES1-5A10 monoclonal antibody specifically binds to the human Interleukin-5 (IL-5). IL-5 is a cytokine that is also known as T-cell replacing factor (TRF), Eosinophil differentiation factor (EDF), and B cell differentiation factor I. IL-5 is produced by T cells, mast cells, and eosinophils. IL-5 controls the generation, differentiation, activation and localization of eosinophils. IL-5 also activates and promotes the differentiation of basophils. IL-5 plays an important role in regulating cell-mediated immunity against parasitic infections and some tumors. High level IL-5 production is involved in some allergic diseases including asthma. IL-5 exerts its biological effects by binding to and signaling through high-affinity heterodimeric IL-5 receptors comprised of transmembrane IL-5 receptor alpha (IL-5Ralpha, aka, CD125) and beta common (betac, aka, CD131) chains. The betac chain serves as the common signaling subunit for the IL-5 receptor heterodimer as well as for the heterodimeric IL-3 and GM-CSF receptors. The immunogen used to generate the JES1-5A10 hybridoma was recombinant human IL-5. The JES1-5A10 antibody has been reported to cross react with IL-5 from rhesus monkey. This is a neutralizing antibody.
Synonyms: EDF, Eosinophil differentiation factor, TRF, T-cell replacing factor
Research Area Cancer
Pathways JAK-STAT Signaling, Positive Regulation of Peptide Hormone Secretion

Application Details

Product Details anti-IL5 Antibody Target Details IL5 Handling References for anti-IL5 Antibody (ABIN1177282) Images back to top
Restrictions For Research Use only

Handling

Product Details anti-IL5 Antibody Target Details IL5 Application Details References for anti-IL5 Antibody (ABIN1177282) Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.
Preservative Azide free
Storage 4 °C
Storage Comment Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

References for anti-IL5 Antibody (ABIN1177282)

Product Details anti-IL5 Antibody Target Details IL5 Application Details Handling Images back to top
Product cited in:

Abrams, Roncarolo, Yssel, Andersson, Gleich, Silver: "Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples." in: Immunological reviews, Vol. 127, Issue 9-10, pp. 5-24, 1992

Butterfield, Leiferman, Abrams, Silver, Bower, Gonchoroff, Gleich: "Elevated serum levels of interleukin-5 in patients with the syndrome of episodic angioedema and eosinophilia." in: Blood, Vol. 79, Issue 3, pp. 688-92, 1992