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anti-Human IL5 antibody for ELISpot (Detection)

Recommended IL5 Antibody (supplied by: Log in to see )

Antigen
Interleukin 5 (IL5) Antibodies
  • EDF
  • IL-5
  • Il-5
  • IL5
  • Pro-TRH
  • TRF
Reactivity
Human
167
131
20
17
17
2
1
1
1
1
Host
Rat
127
95
19
2
Clonality
Monoclonal
Conjugate
This IL5 antibody is conjugated to Biotin
41
12
5
5
4
4
4
3
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
Application
ELISA (Detection), ELISpot (Detection) (ELISPOT (Detection))
124
121
92
34
17
14
11
11
11
9
9
9
8
6
5
4
4
4
3
1
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Catalog No. ABIN279975
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Top referenced anti-IL5 antibody for ELISpot (Detection)

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Immunoprecipitation (IP) 4 Antibodies

General

Antigen Interleukin 5 (IL5) Antibodies
Reactivity Human
(167), (131), (20), (17), (17), (2), (1), (1), (1), (1)
Host Rat
(127), (95), (19), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This IL5 antibody is conjugated to Biotin
(41), (12), (5), (5), (4), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Application ELISA (Detection), ELISpot (Detection) (ELISPOT (Detection))
(124), (121), (92), (34), (17), (14), (11), (11), (11), (9), (9), (9), (8), (6), (5), (4), (4), (4), (3), (1)
Pubmed 1 reference available
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Product Details anti-IL5 Antibody

Target Details IL5 Application Details Handling References for anti-IL5 Antibody (ABIN279975) Images
Specificity The JES1-5A10 antibody reacts with human interleukin-5 (IL-5), also known as BCDF-α and eosinophil differentiation factor (EDF). This 15-20 kDa cytokine is secreted by T cells and exists a homodimer.
Characteristics The JES1-5A10 antibody reacts with human interleukin-5 (IL-5), also known as BCDF-α and eosinophil differentiation factor (EDF). This 15-20 kDa cytokine is secreted by T cells and exists a homodimer.
Synonyms: Synonyms: Interleukin-5, IL5
Clone JES1-5A10
Isotype IgG2a kappa

Target Details IL5

Product Details anti-IL5 Antibody Application Details Handling References for anti-IL5 Antibody (ABIN279975) Images back to top
Antigen
Alternative Name IL-5 (IL5 Antibody Abstract)
Research Area Cytokines, Immunology, Virology, Inflammation, Cancer
Pathways JAK-STAT Signaling

Application Details

Product Details anti-IL5 Antibody Target Details IL5 Handling References for anti-IL5 Antibody (ABIN279975) Images back to top
Application Notes Applications reported: JES1-5A10 has been reported for use in detection of human IL-5 by ELISA.

Applications tested: The JES1-5A10 antibody has been tested as the detection antibody in a sandwich ELISA for analysis of human Interleukin-5 (IL-5) in combination with the purified TRFK5 (14-7052) antibody for capture and recombinant human IL-5 (14-8059) as the standard. A suitable range of concentrations of this antibody for ELISA detection is 0.5-2 μg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 500 pg/mL - 4 pg/mL should be included in each ELISA plate.
Restrictions For Research Use only

Handling

Product Details anti-IL5 Antibody Target Details IL5 Application Details References for anti-IL5 Antibody (ABIN279975) Images back to top
Concentration 0.5 mg/mL
Buffer aqueous buffer, 0.09 % sodium azide, may contain carrier protein/stabilizer
Handling Advice Do not freeze.
Storage 4 °C

References for anti-IL5 Antibody (ABIN279975)

Product Details anti-IL5 Antibody Target Details IL5 Application Details Handling Images back to top
Background publications

Abrams, Roncarolo, Yssel, Andersson, Gleich, Silver: "Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples." in: Immunological reviews, Vol. 127, Issue 9-10, pp. 5-24, 1992