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anti-Human IL5 antibody for Western Blotting

Recommended IL5 Antibody (supplied by: Log in to see )

Antigen
Interleukin 5 (IL5) Antibodies
  • EDF
  • IL-5
  • Il-5
  • IL5
  • Pro-TRH
  • TRF
Epitope
Middle Region
18
6
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Human
167
131
20
17
17
2
1
1
1
1
Host
Rabbit
128
94
19
2
Clonality
Polyclonal
Conjugate
This IL5 antibody is un-conjugated
42
12
5
5
4
4
4
3
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
Application
Western Blotting (WB)
124
120
92
34
17
14
11
11
11
9
9
9
8
6
5
4
4
4
4
1
1
Supplier
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Catalog No. ABIN2774069
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.13341247 ABIN2785670 WB Rabbit Middle Region Log in to see Polyclonal
0.13341247 ABIN2706393 WB Rabbit Center Log in to see Polyclonal
0.13341247 ABIN2706392 IP IHC WB Rabbit Center Log in to see Polyclonal
0.13341247 ABIN498493 IHC (p) WB Rabbit Log in to see Polyclonal
0.13341247 ABIN3185179 ELISA IHC WB Rabbit IgG Internal Region Log in to see Polyclonal
0.13341247 ABIN1868680 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 20-134 Log in to see Polyclonal
0.13341247 ABIN928720 WB Rabbit Middle Region Log in to see Polyclonal
0.13341247 ABIN634806 WB Rabbit Log in to see Polyclonal
0.0011728023 ABIN118519 EIA FACS WB Rat IgG1 Log in to see TRFK5 4
0.0011728023 ABIN2663800 FACS ELISA WB PE Rat IgG2a kappa Log in to see JES1-39D10 9
0.0011728023 ABIN322076 WB Rabbit IgG Internal Region Log in to see Polyclonal
0.0011728023 ABIN116078 EIA WB Biotin Rabbit Log in to see Polyclonal
0.0011728023 ABIN116077 EIA WB Biotin Rabbit Log in to see Polyclonal
0.0011728023 ABIN233214 ELISA WB Rabbit IgG Log in to see Polyclonal
0.0011728023 ABIN2475028 ELISA WB Biotin Rabbit IgG Log in to see Polyclonal 2
0.0011728023 ABIN116080 EIA Func IHC (p) WB Rabbit Log in to see Polyclonal
0.0011728023 ABIN116079 EIA Func IHC (p) WB Rabbit Log in to see Polyclonal
0.0011728023 ABIN674904 IF (p) IHC (p) WB Rabbit IgG Log in to see Polyclonal
0.0011728023 ABIN243704 ELISA WB Rat IgG Log in to see SPM462
0.0011728023 ABIN1822026 ELISA WB Biotin Rabbit IgG Log in to see Polyclonal

Top referenced anti-IL5 antibody for Western Blotting

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Application / Reactivity Human
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ELISA 82 Antibodies
ELISA (Capture) 4 Antibodies
ELISA (Detection) 3 Antibodies
ELISpot 5 Antibodies
ELISpot (Capture) (ELISPOT (Capture)) 1 Antibodies
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Enzyme Immunoassay (EIA) 7 Antibodies
Flow Cytometry (FACS) 61 Antibodies
Functional Studies (Func) 9 Antibodies
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Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
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Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 8 Antibodies
Immunoprecipitation (IP) 4 Antibodies

General

Antigen Interleukin 5 (IL5) Antibodies
Epitope Middle Region
(18), (6), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(167), (131), (20), (17), (17), (2), (1), (1), (1), (1)
Host Rabbit
(128), (94), (19), (2)
Clonality
Polyclonal
Conjugate This IL5 antibody is un-conjugated
(42), (12), (5), (5), (4), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Application Western Blotting (WB)
(124), (120), (92), (34), (17), (14), (11), (11), (11), (9), (9), (9), (8), (6), (5), (4), (4), (4), (4), (1), (1)
Pubmed 1 reference available
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Product Details anti-IL5 Antibody

Target Details IL5 Application Details Handling References for anti-IL5 Antibody (ABIN2774069) Images
Blocking Peptide Blocking peptide for this product available: ABIN978514
Sequence LESQTVQGGT VERLFKNLSL IKKYIDGQKK KCGEERRRVN QFLDYLQEFL
Predicted Reactivity Human : 100 %
Characteristics This is a rabbit polyclonal antibody against IL5. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the middle region of human IL5

Target Details IL5

Product Details anti-IL5 Antibody Application Details Handling References for anti-IL5 Antibody (ABIN2774069) Images back to top
Antigen
Alternative Name IL5 (IL5 Antibody Abstract)
Background IL5 is a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. This cytokine is a main regulator of eosinopoiesis, eosinophil maturation and activation. The elevated production of this cytokine is reported to be related to asthma or hypereosinophilic syndromes. The protein encoded by this gene is a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. This cytokine is a main regulator of eosinopoiesis, eosinophil maturation and activation. The elevated production of this cytokine is reported to be related to asthma or hypereosinophilic syndromes. The receptor of this cytokine is a heterodimer, whose beta subunit is shared with the receptors for interleukine 3 (IL3) and colony stimulating factor 2 (CSF2/GM-CSF). This gene, together with those for interleukin 4 (IL4), interleukin 13 (IL13), and CSF2, form a cytokine gene cluster on chromosome 5. This cytokine, IL4, and IL13 are found to be regulated coordinately by long-range regulatory elements spread over 120 kilobases on chromosome 5q31.
Molecular Weight 13 kDa
Gene ID 3567
NCBI Accession NM_000879, NP_000870
UniProt P05113
Research Area Cancer, Cytokines, Innate Immunity, Adaptive Immunity
Pathways JAK-STAT Signaling

Application Details

Product Details anti-IL5 Antibody Target Details IL5 Handling References for anti-IL5 Antibody (ABIN2774069) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 134 AA

Restrictions For Research Use only

Handling

Product Details anti-IL5 Antibody Target Details IL5 Application Details References for anti-IL5 Antibody (ABIN2774069) Images back to top
Format Liquid
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C,-20 °C
Storage Comment For short term use, store at 2-8 °C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-IL5 Antibody (ABIN2774069)

Product Details anti-IL5 Antibody Target Details IL5 Application Details Handling Images back to top
Background publications

Chong, Aicheler, Llewellyn-Lacey, Tomasec, Brennan, Wang: "Proliferation and interleukin 5 production by CD8hi CD57+ T cells." in: European journal of immunology, Vol. 38, Issue 4, pp. 995-1000, 2008

Images

Product Details anti-IL5 Antibody Target Details IL5 Application Details Handling References for anti-IL5 Antibody (ABIN2774069) back to top
Supplier Images
Western Blotting (WB) image for anti-IL5 antibody (Interleukin 5) (Middle Region) (ABIN2774069) WB Suggested Anti-IL5 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1...