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anti-Human IL5 antibody for Western Blotting

Recommended IL5 Antibody (supplied by: Log in to see )

Antigen
Interleukin 5 (IL5) Antibodies
  • IL5
  • Il-5
  • IL-5
  • TRF
  • EDF
  • Pro-TRH
  • interleukin 5
  • Interleukin 5
  • interleukin 5 (colony-stimulating factor, eosinophil)
  • interleukin-5-like
  • thyrotropin-releasing hormone
  • IL5
  • Il5
  • IL-5
  • LOC100358075
  • TRH
Epitope
Middle Region
28
4
4
3
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Human
173
131
27
20
17
3
2
2
1
1
1
Host
Rabbit
114
100
24
2
Clonality
Polyclonal
Conjugate
This IL5 antibody is un-conjugated
36
14
5
5
4
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
122
122
93
23
22
13
11
10
9
9
9
7
6
6
6
4
4
2
2
1
1
1
Options
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Catalog No. ABIN2774069
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.0882466 ABIN2785670 WB Rabbit Middle Region Log in to see Polyclonal
3.0882466 ABIN446956 IHC (p) IHC WB Rabbit Log in to see Polyclonal 1
3.0882466 ABIN3185179 ELISA IHC WB Rabbit IgG Internal Region Log in to see Polyclonal
3.0882466 ABIN2706392 IP IHC WB Rabbit Center Log in to see Polyclonal
3.0882466 ABIN2706393 WB Rabbit Center Log in to see Polyclonal
3.0882466 ABIN498493 IHC (p) WB Rabbit Log in to see Polyclonal
3.0882466 ABIN2736976 IHC WB Rabbit IgG Log in to see Polyclonal
3.0882466 ABIN1868680 ICC IHC IP WB Rabbit IgG AA 20-134 Log in to see Polyclonal
3.0882466 ABIN4892226 WB Rabbit Log in to see Polyclonal
3.0882466 ABIN928720 WB Rabbit Middle Region Log in to see Polyclonal
3.0882466 ABIN634806 WB Rabbit Log in to see Polyclonal
1 ABIN610960 WB Rabbit AA 21-70 Log in to see Polyclonal 1
1 ABIN4900611 FACS ICC WB Mouse IgG1 AA 20-134 Log in to see 9906 2
1 ABIN118519 EIA FACS WB Rat IgG1 Log in to see TRFK5 4
1 ABIN2663800 FACS ELISA WB PE Rat IgG2a kappa Log in to see JES1-39D10 9
1 ABIN322076 WB Rabbit IgG AA 74-123 Log in to see Polyclonal
1 ABIN116078 EIA WB Biotin Rabbit Log in to see Polyclonal
1 ABIN116077 EIA WB Biotin Rabbit Log in to see Polyclonal
1 ABIN2475028 ELISA WB Biotin Rabbit IgG Log in to see Polyclonal 2
1 ABIN465376 ELISA IHC WB Biotin Rabbit Log in to see Polyclonal

Top referenced anti-IL5 antibody for Western Blotting

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Application / Reactivity Human
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ELISA (Capture) 4 Antibodies
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General

Antigen Interleukin 5 (IL5) Antibodies
Epitope Middle Region
(28), (4), (4), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(173), (131), (27), (20), (17), (3), (2), (2), (1), (1), (1)
Host Rabbit
(114), (100), (24), (2)
Clonality
Polyclonal
Conjugate This IL5 antibody is un-conjugated
(36), (14), (5), (5), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Western Blotting (WB)
(122), (122), (93), (23), (22), (13), (11), (10), (9), (9), (9), (7), (6), (6), (6), (4), (4), (2), (2), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-IL5 Antibody

Target Details IL5 Application Details Handling References for anti-IL5 Antibody (ABIN2774069) Images
Blocking Peptide Blocking peptide for this product available: ABIN978514
Sequence LESQTVQGGT VERLFKNLSL IKKYIDGQKK KCGEERRRVN QFLDYLQEFL
Predicted Reactivity Human: 100%
Characteristics This is a rabbit polyclonal antibody against IL5. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the middle region of human IL5

Target Details IL5

Product Details anti-IL5 Antibody Application Details Handling References for anti-IL5 Antibody (ABIN2774069) Images back to top
Antigen
Alternative Name IL5 (IL5 Antibody Abstract)
Background IL5 is a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. This cytokine is a main regulator of eosinopoiesis, eosinophil maturation and activation. The elevated production of this cytokine is reported to be related to asthma or hypereosinophilic syndromes. The protein encoded by this gene is a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. This cytokine is a main regulator of eosinopoiesis, eosinophil maturation and activation. The elevated production of this cytokine is reported to be related to asthma or hypereosinophilic syndromes. The receptor of this cytokine is a heterodimer, whose beta subunit is shared with the receptors for interleukine 3 (IL3) and colony stimulating factor 2 (CSF2/GM-CSF). This gene, together with those for interleukin 4 (IL4), interleukin 13 (IL13), and CSF2, form a cytokine gene cluster on chromosome 5. This cytokine, IL4, and IL13 are found to be regulated coordinately by long-range regulatory elements spread over 120 kilobases on chromosome 5q31. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: EDF, IL-5, TRF
Protein Interaction Partner: CXCL8, IL5RA, IL5, STAT5A,
Protein Size: 134
Molecular Weight 13 kDa
Gene ID 3567
NCBI Accession NM_000879, NP_000870
UniProt P05113
Research Area Cancer, Cytokines, Innate Immunity, Adaptive Immunity
Pathways JAK-STAT Signaling, Positive Regulation of Peptide Hormone Secretion, Production of Molecular Mediator of Immune Response, Feeding Behaviour

Application Details

Product Details anti-IL5 Antibody Target Details IL5 Handling References for anti-IL5 Antibody (ABIN2774069) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 134 AA

Restrictions For Research Use only

Handling

Product Details anti-IL5 Antibody Target Details IL5 Application Details References for anti-IL5 Antibody (ABIN2774069) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-IL5 Antibody (ABIN2774069)

Product Details anti-IL5 Antibody Target Details IL5 Application Details Handling Images back to top
Background publications

Chong, Aicheler, Llewellyn-Lacey, Tomasec, Brennan, Wang: "Proliferation and interleukin 5 production by CD8hi CD57+ T cells." in: European journal of immunology, Vol. 38, Issue 4, pp. 995-1000, 2008

Images

Product Details anti-IL5 Antibody Target Details IL5 Application Details Handling References for anti-IL5 Antibody (ABIN2774069) back to top
Supplier Images
Western Blotting (WB) image for anti-IL5 antibody (Interleukin 5) (Middle Region) (ABIN2774069) WB Suggested Anti-IL5 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1...