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|Application / Reactivity||Human|
|Blocking Antibody (Inhibition)||4 Antibodies|
|Dot Blot (DB)||2 Antibodies|
|ELISA (Capture)||2 Antibodies|
|ELISA (Detection)||2 Antibodies|
|Enzyme Immunoassay (EIA)||8 Antibodies|
|Flow Cytometry (FACS)||1 Antibodies|
|Functional Studies (Func)||9 Antibodies|
|Immunoassay (IA)||2 Antibodies|
|Immunocytochemistry (ICC)||4 Antibodies|
|Immunofluorescence (IF)||5 Antibodies|
|Immunohistochemistry (IHC)||32 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||1 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||6 Antibodies|
|Immunoprecipitation (IP)||16 Antibodies|
|Intracellular Flow Cytometry (ICFC)||5 Antibodies|
|Intracellular Staining (ICS)||2 Antibodies|
|Neutralization (Neut)||29 Antibodies|
|Antigen||Interleukin 7 (IL7) Antibodies|
|Conjugate||This IL7 antibody is un-conjugated Alternatives|
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
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Product Details anti-IL7 AntibodyTarget Details IL7 Application Details Handling Images
Target Details IL7Product Details anti-IL7 Antibody Application Details Handling Images back to top
|Alternative Name||Interleukin-7 / IL7 (IL7 Antibody Abstract)|
|Background||Gene Symbol: IL7|
Application DetailsProduct Details anti-IL7 Antibody Target Details IL7 Handling Images back to top
By direct ELISA, 1:10,000 dilution will yield 0.3 O.D using alkaline phosphatase conjugated rabbit anti-mouse Ig
Recommended dilutions: ELISA 1:100-1:2000, Immunoprecipitation 1:10-1:500, Western Blot 1:100-1:2000, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunohistochemistry-Paraffin 1:10-1:500
Protocol specific for IL7 Antibody IHC-Paraffin and IHC-Frozen ProtocolDAY 1:I. Preparation of slides:1) Deparaffinization: soak tissue slides with xylene twice, each time for 15 minutes2) Rehydration: incubate slides in the following graded series of ethanol: 100 %I, 100 %II, 95 %, 90 %, 80 %, 70 %. 5 minutes for each solution. Then incubate in the water for 5 minutes.Note: The step 1 is only for paraffin embedded slide. For frozen slide, soak the slide in water for 5 minutes, and go to step 2.II. Protocol for Immunostaining:1) Immerse the slides in 0.3 % H2O2 (in distilled water) for 30 minutes at room temperature2) Rinse the slides with water followed by 1x PBS (pH7.
4) once, circle the tissue section with a Pap Pen (or Para-Pen, ImmEdge Pen)3) Incubate the slide with 1 % Normal serum/PBS [Mix 3.5 mL 1x PBS, pH 7.4 with 1 drops (about 35 µL/drop) of normal serum in a tube] for 30 minutes at room temperature4) Drop off the normal serum from the slides5) Incubate the sections with the PBS diluted first antibody (optimize the antibody titer before starting IHC) in a humid chamber for 1 hour to overnight at room temperature.DAY 2:6) Rinse the slides with 1xPBS for 3 times, each time for 5 minutes7) Incubate the slides with PBS diluted Biotin-labeled secondary antibody for 30 minutes at room temperature. [Mix 1.4 mL 1xPBS pH 7.4 and 1 drop (about 35 µL/drop) of biotinylated anti-mouse IgG in a tube]8) Rinse the slides with 1xPBS for 3 times, each time for 5 minutes9) Preparation of detection solution: Mix 1.33 mL 1xPBS, 1 drops (about 35 µL/drop) of Solution A, and 1 drops (about 35 µL/drop) of Solution B in a tube. Incubate the mixture at room temperature for 30 minutes before use.
) Add the detection solution prepared at step 9) to the tissue section. Incubate room temperature for 30 minutes.
) Rinse the slides with 1xPBS for 3 times, each time for 5 minutes.
) Make fresh Development solution: Mix 1.6 mL DAB buffer, and 1 drop (about 35 µL/drop) of Liquid DAB in a tube.
) Add the Development solution to cover the tissues, and develop for 5-30 minutes14) Stop the reaction by soak the tissue in water.
) Counter stain the slides if necessary (Use Harris' Hematoxylin to stain the nuclear, and use eosin Y to stain cytosol)16) Dehydrate by soaked in the graded series of alcohol: 70 %-80 %-90 %-95 %-100 %I-100 %II, 3 minutes for each solution, then incubated in Xylene twice, 10 minutes each.
) Mounting the slides (Fisher's permount Cat. # SP15-100 or similar mounting material can be used)18) Add 2 drops (about 35 µL/drop) of detoxification solution into the used development solution. Incubate for 1 hour or overnight. Then it can be discarded.
|Restrictions||For Research Use only|
HandlingProduct Details anti-IL7 Antibody Target Details IL7 Application Details Images back to top
|Buffer||Supplied in PBS, Sodium Azide|
|Precaution of Use||WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.|
|Handling Advice||Do not freeze.|