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Human IL7R ELISA Kit

Recommended IL7R Product (supplied by: Log in to see )

Antigen
Interleukin 7 Receptor (IL7R) ELISA Kits
  • CD127
  • CDW127
  • IL-7R-alpha
  • IL-7RA
  • IL-7Ralpha
  • IL7R
  • IL7RA
  • ILRA
Epitope
AA 21-262
Reactivity
Human
Alternatives
Kits with alternative reactivity to:
9
8
3
2
1
1
1
Methode Type
Sandwich ELISA
Detection Range
312-20000 pg/mL
Minimum Detection Limit
312 pg/mL
Application
ELISA
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Catalog No. ABIN1889315
$ 369.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
0.30647367 ABIN417763 Sandwich ELISA Serum, Plasma, Biological Fluids 1.56-100 ng/mL 1.56 ng/mL Log in to see
0.30647367 ABIN1570753 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 1.563-100 ng/mL 1.563 ng/mL Log in to see
0.30647367 ABIN2708118 Sandwich ELISA Cell Culture Supernatant, Serum 312-20000 pg/mL 312 pg/mL Log in to see
0.0012153484 ABIN1380460 Cell ELISA Log in to see
0.0012153484 ABIN2684731 Sandwich ELISA Cell Culture Supernatant, Serum 312-20000 pg/mL 312 pg/mL Log in to see
0.0012153484 ABIN2113406 Cell ELISA Log in to see
0.0012153484 ABIN1138708 0.312-20 ng/mL 0.312 ng/mL Log in to see
0.0012153484 ABIN2535643 Sandwich ELISA Serum, Plasma, Biological Fluids 1.56-100 ng/mL 1.56 ng/mL Log in to see
0.0012153484 ABIN2948920 1.563-100 ng/mL 1.563 ng/mL Log in to see

Top referenced IL7R ELISA Kit for ELISA

  • Human IL7R ELISA Kit for Sandwich ELISA - ABIN1889315 (1 Publications): Liu, Leung, Wang, Tan, Wang, Guo, Fang, Zhao, Wan, Qin, Lu, Li, Pan, Song, Liu, Hong, Lu, Zhang: Crucial role of interleukin-7 in T helper type 17 survival and expansion in autoimmune disease. in Nature medicine 2010 (PubMed)

Similar IL7R ELISA Kits

Application / Reactivity Human
ELISA 10 ELISA Kits

General

Antigen Interleukin 7 Receptor (IL7R) ELISA Kits
Epitope AA 21-262
Reactivity Human
Kits with alternative reactivity to:
(9), (8), (3), (2), (1), (1), (1)
Methode Type Sandwich ELISA
Detection Range 312-20000 pg/mL
Minimum Detection Limit 312 pg/mL
Application ELISA
Pubmed 1 reference available
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Product Details IL7R ELISA Kit

Target details Application Details Handling References for IL7R Kit (ABIN1889315) Images
Purpose For quantitative detection of human IL7R in cell culture supernates and serum.
Brand PicoKine™
Sample Type Cell Culture Supernatant, Serum
Detection Method Colorimetric
Specificity Natural and recombinant human IL7R
Cross-Reactivity (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivity <10pg/mL
Components
  • 96-well plate precoated with anti- human IL7R antibod - 1
  • Lyophilized recombinant human IL7R standar - 20ng/tubex2
  • Biotinylated anti- human IL7R antibod - 130μl(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex (ABC - 130μl(dilution 1:100)
  • Sample diluent buffe - 30 ml
  • Antibody diluent buffe - 12ml
  • ABC diluent buffe - 12ml
  • TMB color developing agen - 10ml
  • TMB stop solutio - 10ml
Material not included Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: NSO
Immunogen sequence: E21-I262

Target details

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Antigen
Alternative Name IL7R (IL7R ELISA Kit Abstract)
Background The interleukin-7 receptor(IL7R) is a protein found on the surface of cells. It is mapped to 5p13.2. The protein encoded by this gene is a receptor for interleukine 7(IL7). The function of this receptor requires the interleukin 2 receptor, gamma chain(IL2RG), which is a common gamma chain shared by the receptors of various cytokines, including interleukine 2, 4, 7, 9, and 15. IL7R has been shown to play a critical role in the development of immune cells called lymphocytes-specifically in a process known as V(D)J recombination. This protein is also found to control the accessibility of a region of the genome that contains the T-cell receptor gamma gene, by STAT5 and histone acetylation. What's more, IL7R antagonism is efficacious in treatment of EAE through its effects on Th17 cells and is a potential treatment for MS.
Gene ID 3575
UniProt P16871
Research Area Immunology
Pathways JAK-STAT Signaling

Application Details

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Application Notes Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Plate Pre-coated,Strips (12 x 8)
Protocol human IL7R ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL7R has been precoated onto 96-well plates. Standards(NSO, E21-I262) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL7R is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL7R amount of sample captured in plate.
Assay Procedure Aliquot 0.1 mL per well of the 20000pg/mL, 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL human IL7R standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates or serum to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human IL7R standard solution and each sample be measured in duplicate.
Assay Precision
  • Sample 1: n=16, Mean(ng/ml): 2.8, Standard deviation: 0.168, CV(%): 6
  • Sample 2: n=16, Mean(ng/ml): 8, Standard deviation: 0.36, CV(%): 4.5
  • Sample 3: n=16, Mean(ng/ml): 14.3, Standard deviation: 0.815, CV(%): 5.7,
  • Sample 1: n=24, Mean(ng/ml): 3.4, Standard deviation: 0.245, CV(%): 7.2
  • Sample 2: n=24, Mean(ng/ml): 9.1, Standard deviation: 0.51, CV(%): 5.6
  • Sample 3: n=24, Mean(ng/ml): 16.2, Standard deviation: 1.07, CV(%): 6.6
Restrictions For Research Use only

Handling

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Handling Advice Avoid multiple freeze-thaw cycles
Storage -20 °C,4 °C
Storage Comment Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date 12 months

References for IL7R Kit (ABIN1889315)

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Background publications

Liu, Leung, Wang, Tan, Wang, Guo, Fang, Zhao, Wan, Qin, Lu, Li, Pan, Song, Liu, Hong, Lu, Zhang: "Crucial role of interleukin-7 in T helper type 17 survival and expansion in autoimmune disease." in: Nature medicine, Vol. 16, Issue 2, pp. 191-7, 2010

Images

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Supplier Images
ELISA image for IL7R ELISA Kit (Interleukin 7 Receptor) (ABIN1889315) Human IL7R/CD127 PicoKine ELISA Kit standard curve