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Mouse (Murine) IL7R ELISA Kit

Recommended IL7R Product (supplied by: Log in to see )

Antigen
Interleukin 7 Receptor (IL7R) ELISA Kits
  • CD127
  • CDW127
  • IL-7R-alpha
  • IL-7RA
  • IL-7Ralpha
  • IL7R
  • IL7RA
  • ILRA
Epitope
AA 21-239
Reactivity
Mouse (Murine)
Alternatives
Kits with alternative reactivity to:
10
7
3
2
1
1
1
Methode Type
Sandwich ELISA
Detection Range
312-20000 pg/mL
Minimum Detection Limit
312 pg/mL
Application
ELISA
Supplier
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Catalog No. ABIN2859286
$ 369.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
0.0012104211 ABIN842030 Sandwich ELISA Serum, Plasma, Tissue Homogenate 8000-125 pg/mL 8000 pg/mL Log in to see
0.0012104211 ABIN1380460 Cell ELISA Log in to see
0.0012104211 ABIN2748768 Sandwich ELISA Cell Culture Supernatant, Serum 312-20000 pg/mL 312 pg/mL Log in to see
0.0012104211 ABIN2684732 Sandwich ELISA Cell Culture Supernatant, Serum 312-20000 pg/mL 312 pg/mL Log in to see
0.0012104211 ABIN2113406 Cell ELISA Log in to see
0.0012104211 ABIN1138709 Log in to see

Similar IL7R ELISA Kits

Application / Reactivity Mouse (Murine)
ELISA 7 ELISA Kits

General

Antigen Interleukin 7 Receptor (IL7R) ELISA Kits
Epitope AA 21-239
Reactivity Mouse (Murine)
Kits with alternative reactivity to:
(10), (7), (3), (2), (1), (1), (1)
Methode Type Sandwich ELISA
Detection Range 312-20000 pg/mL
Minimum Detection Limit 312 pg/mL
Application ELISA
Supplier Log in to see

Product Details IL7R ELISA Kit

Target details Application Details Handling Images
Purpose For quantitative detection of mouse IL7R in cell culture supernates and serum.
Brand PicoKine™
Sample Type Cell Culture Supernatant, Serum
Detection Method Colorimetric
Specificity Natural and recombinant mouse IL7R
Cross-Reactivity (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivity <10pg/mL
Components
  • 96-well plate precoated with anti- mouse IL7R antibod - 1
  • Lyophilized recombinant mouse IL7R standar - 20ng/tubex2
  • Biotinylated anti- mouse IL7R antibod - 130μl(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex (ABC - 130μl(dilution 1:100)
  • Sample diluent buffe - 30 ml
  • Antibody diluent buffe - 12ml
  • ABC diluent buffe - 12ml
  • TMB color developing agen - 10ml
  • TMB stop solutio - 10ml
Immunogen Expression system for standard: NSO
Immunogen sequence: E21-D239

Target details

Product Details IL7R ELISA Kit Application Details Handling Images back to top
Antigen
Alternative Name IL7R (IL7R ELISA Kit Abstract)
Background The interleukin-7 receptor(IL7R) is a protein found on the surface of cells. It is mapped to 5p13.2. The protein encoded by this gene is a receptor for interleukine 7(IL7). The function of this receptor requires the interleukin 2 receptor, gamma chain(IL2RG), which is a common gamma chain shared by the receptors of various cytokines, including interleukine 2, 4, 7, 9, and 15. IL7R has been shown to play a critical role in the development of immune cells called lymphocytes-specifically in a process known as V(D)J recombination. This protein is also found to control the accessibility of a region of the genome that contains the T-cell receptor gamma gene, by STAT5 and histone acetylation. What's more, IL7R antagonism is efficacious in treatment of EAE through its effects on Th17 cells and is a potential treatment for MS.
Gene ID 16197
UniProt P16872
Research Area Immunology
Pathways JAK-STAT Signaling

Application Details

Product Details IL7R ELISA Kit Target details Handling Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Plate Pre-coated,Strips (12 x 8)
Protocol mouse IL7R ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IL7R has been precoated onto 96-well plates. Standards(NSO, E21-D239) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL7R is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse IL7R amount of sample captured in plate.
Assay Procedure Aliquot 0.1 mL per well of the 20000pg/mL, 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL mouse IL7R standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates or serum to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse IL7R standard solution and each sample be measured in duplicate.
Assay Precision
  • Sample 1: n=16, Mean(ng/ml): 2, Standard deviation: 0.114, CV(%): 5.7
  • Sample 2: n=16, Mean(ng/ml): 6.5, Standard deviation: 0.442, CV(%): 6.8
  • Sample 3: n=16, Mean(ng/ml): 12.8, Standard deviation: 0.576, CV(%): 4.5,
  • Sample 1: n=24, Mean(ng/ml): 2.3, Standard deviation: 0.145, CV(%): 6.3
  • Sample 2: n=24, Mean(ng/ml): 7.3, Standard deviation: 0.555, CV(%): 7.6
  • Sample 3: n=24, Mean(ng/ml): 14.5, Standard deviation: 0.725, CV(%): 5
Restrictions For Research Use only

Handling

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Storage -20 °C,4 °C
Storage Comment Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date 12 months

Images

Product Details IL7R ELISA Kit Target details Application Details Handling back to top
Supplier Images
ELISA image for IL7R ELISA Kit (Interleukin 7 Receptor) (ABIN2859286) Mouse IL7R/CD127 PicoKine ELISA Kit standard curve