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|Antigen||Interleukin 9 (IL9) Antibodies|
|Reactivity||Mouse (Murine) Alternatives|
|Conjugate||This IL9 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Functional Studies (Func), Western Blotting (WB)
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Product Details anti-IL9 AntibodyTarget Details IL9 Application Details Handling Images
|Specificity||Recognises Murine IL-9|
|Immunogen||Highly pure (>98%) recombinant mIL-9|
Target Details IL9Product Details anti-IL9 Antibody Application Details Handling Images back to top
|Alternative Name||Interleukin-9 / IL9 (IL9 Antibody Abstract)|
|Background||Interleukin 9 (IL9) is a cytokine preferentially produced by T helper type 2 lymphocytes and active on various cell types such as T and B lymphocytes, mast cells and haemopoietic progenitors, but no effect of this cytokine has been reported so far on mononuclear phagocytes. IL9 stimulates cell proliferation and prevents apoptosis. The IL9 receptor (IL 9R) belongs to the haemopoietic receptor superfamily and its signal transduction involves mainly the (JAK/STAT) pathway. Some studies have implicated IL9 as an important cytokine in a number of Th2 cytokine mediated pathologies, in particular asthma.Synonyms: IL-9, P40 cytokine, T-cell growth factor P40|
Application DetailsProduct Details anti-IL9 Antibody Target Details IL9 Handling Images back to top
Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of mIL-9 (0.30 ng/mL),a concentration of 0.006-0.012 μg/mL of this antibody is required. ELISA: To detect mIL-9 by direct ELISA (using 100 l/well antibody solution) a concentration of atleast 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, inconjunction with compatible secondary reagents, allows the detection of 0.2-0.4 ng/wellof recombinant mIL-9. Western Blot: To detect mIL-9 by Western Blot analysis this antibody can be used at a concentration of0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limitfor recombinant mIL-9 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-IL9 Antibody Target Details IL9 Application Details Images back to top
|Reconstitution||Restore in sterile water to a concentration of 0.1-1.0 mg/mL.|
|Buffer||PBS, pH 7.2 without preservatives.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.|