You are viewing an incomplete version of our website. Please click to reload the website as full version.

Mouse (Murine) Leptin ELISA Kit

Recommended Leptin Product (supplied by: Log in to see )

Antigen
Leptin (LEP) ELISA Kits
  • LEPD
  • OB
  • ob
  • obese
  • OBS
Reactivity
Mouse (Murine)
Alternatives
Kits with alternative reactivity to:
48
32
22
15
11
8
7
7
5
5
4
3
3
3
1
1
1
Methode Type
Sandwich ELISA
Detection Range
163.8-40000 pg/mL
Minimum Detection Limit
163.8 pg/mL
Application
ELISA
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Catalog No. ABIN2506965
$ 433.40
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
0.32748017 ABIN415517 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 0.15-10 ng/mL 0.15 ng/mL Log in to see 3
0.32748017 ABIN1115745 Sandwich ELISA Serum, Plasma, Biological Fluids 62.5-4000 pg/mL 62.5 pg/mL Log in to see 1
0.32748017 ABIN924822 Sandwich ELISA Cell Lysate, Serum, Plasma 20-1250 pg/mL 20 pg/mL Log in to see
0.32748017 ABIN1029097 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 0.156-10 ng/mL 0.156 ng/mL Log in to see
0.32748017 ABIN455676 Serum, Plasma, Tissue Homogenate, Cell Culture Supernatant, Biological Fluids 0.312-20 ng/mL 0.312 ng/mL Log in to see
0.32748017 ABIN956062 Log in to see
0.0012092679 ABIN365180 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Culture Supernatant 15.6-1000 pg/mL 15.6 pg/mL Log in to see 5
0.0012092679 ABIN1995061 Sandwich ELISA Serum, Plasma, Biological Fluids 62.5-4000 pg/mL 62.5 pg/mL Log in to see
0.0012092679 ABIN612800 Sandwich ELISA Plasma, Cell Culture Supernatant 300 pg/mL Log in to see 7
0.0012092679 ABIN3032824 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 4.1-1000 pg/mL 4.1 pg/mL Log in to see
0.0012092679 ABIN628075 Competition ELISA Serum, Cell Culture Supernatant, Tissue Homogenate, Plasma, Body Fluids 2.5-50 ng/mL 2.5 ng/mL Log in to see
0.0012092679 ABIN625156 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant Log in to see 17
0.0012092679 ABIN3198593 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 62.50-4000 pg/mL 62.50 pg/mL Log in to see
0.0012092679 ABIN2748314 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 0.2-50 ng/mL 0.2 ng/mL Log in to see
0.0012092679 ABIN1112737 Sandwich ELISA 62.5-4000 pg/mL 62.5 pg/mL Log in to see
0.0012092679 ABIN2114697 Cell Lysate, Serum, Plasma 20-1250 pg/mL 20 pg/mL Log in to see
0.0012092679 ABIN930284 31.25-2000 pg/mL 31.25 pg/mL Log in to see
0.0012092679 ABIN2684854 Sandwich ELISA Plasma (citrate), Plasma (EDTA), Plasma (heparin), Cell Culture Supernatant, Serum 100-4000 pg/mL 100 pg/mL Log in to see
0.0012092679 ABIN1846989 Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 62.5-4000 pg/mL 62.5 pg/mL Log in to see
0.0012092679 ABIN2116183 15.6-1000 pg/mL 15.6 pg/mL Log in to see

Top referenced Leptin ELISA Kit for ELISA

Similar Leptin ELISA Kits

Application / Reactivity Mouse (Murine)
ELISA 29 ELISA Kits

General

Antigen Leptin (LEP) ELISA Kits
Reactivity Mouse (Murine)
Kits with alternative reactivity to:
(48), (32), (22), (15), (11), (8), (7), (7), (5), (5), (4), (3), (3), (3), (1), (1), (1)
Methode Type Sandwich ELISA
Detection Range 163.8-40000 pg/mL
Minimum Detection Limit 163.8 pg/mL
Application ELISA
Supplier Log in to see

Product Details Leptin ELISA Kit

Target details Application Details Handling Images
Purpose The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of leptin in mouse serum, plasma, and other biological fluids not being tested
Brand EasyTest™
Sample Type Serum, Plasma
Detection Method Colorimetric
Specificity This assay has high sensitivity and excellent specificity for detection of mouse leptin.
Sensitivity 49 pg/mL
Characteristics one wash
Components
  • Assay plate (12x8 coated Microwells)-1
  • Standard-2x 10μL
  • mouse leptin Mix-4x 8 μL
  • Assay diluent (5x concentrate)-1 x15mL
  • Wash buffer (20x concentrate)-1x25 mL
  • TMB substrate-1x12mL
  • Stop solution-1x 8mL
  • Adhensive strip-2
  • Instruction manual-1.
Material not included 1. Distilled or deionized water, 2.Precision pipettes, with disposable plastic tips, 3.Beakers, flasks, cylinders necessary for preparation of reagents, 4.Microplate washing device (multichannel pipette or automated microplate washer), 5.Microplate shaker, 6.Microplate reader capable of reading at 450 nm.

Target details

Product Details Leptin ELISA Kit Application Details Handling Images back to top
Antigen
Alternative Name Leptin (LEP ELISA Kit Abstract)
Background Mouse Leptin is a protein product of the mouse obese gene. Mouse Leptin cDNA encodes a 167 aa residue protein with a 21 aa residue signal sequence that is cleaved to yield the 146 aa residue mature protein. cDNA clones encoding Leptin have been isolated from human, simian, mouse and rat cells. Mature mouse Leptin shares approximately 96 % and 84 % amino acid (aa) sequence identity with the rat and human proteins, respectively.
Molecular Weight 16 kDa
Gene ID 16846
NCBI Accession NP_032519
UniProt P41160
Research Area Cardiovascular, Atherosclerosis, Hormones
Pathways JAK-STAT Signaling, AMPK Signaling

Application Details

Product Details Leptin ELISA Kit Target details Handling Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Sample Volume 50 μL
Assay Time 2.5 h
Plate Strips (12 x 8)
Protocol This assay employs the fast quantitative sandwich enzyme immunoassay technique. Antibody specific for mouse leptin has been pre-coated onto a microplate. Standards, samples and mouse leptin mix are pipetted into the wells and any Leptin present is bound by the immobilized antibody. Following a wash to remove any unbound reagent, a TMB substrate solution is added to the wells and color develops in proportion to the amount of leptin bound in the initial step. The color development is stopped and the intensity of the color is measured.
Reagent Preparation 1. Assay diluent: Dilute the concentrated assay diluent 1:5 with distilled water (e.g. 10 mL plus 40 mL). 2. Wash buffer: Dilute the concentrated wash buffer 1:20 with distilled water (e.g. 20 mL plus 380 mL). 3. Standard: Briefly spin standard vial before use. Add 490 μL 1x Assay Diluent to prepare a 100 ng/mL standard. Gently vortex to mix.Take 200 μL mouse leptin standard into a tube, then add 300 μL 1x Assay Diluent to prepare a 40000 pg/mL stock standard solution.Add 150 μL 1x Assay Diluent to 7 tubes. Perform serial dilutions by adding 100 μL of each standard to the next tube and vortexing between each transfer, the last tube with 1x assay diluent is the blank as 0pg/mL. 4. mouse leptin Mix: Within 15 minutes prior to use, briefly spin the vial. Add 1492 μL of 1x Assay diluent to the vial and mix by pipetting. A vial mix can be used for about 30 wells.
Sample Collection Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4 °C before centrifugation for 15 minutes at 1000 x g. Remove serum and assay immediately or aliquot and store samples at -20 °C or -80 °C. Avoid repeated freeze-thaw cycles.
Plasma: Collect plasma using EDTA, or citrate or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 x g at 2-8 °C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20 °C or -80 °C. Avoid repeated freeze-thaw cycles.
Sample Preparation Levels of the target protein may vary among different specimens. Optimal dilution factors for each sample must be determined by the investigator,The dilution scheme is only suggestion: the recommended dilution for serum and plasma is 1:10.
Assay Procedure 1. All reagents must be brought to room temperature (18-25 °C) prior to use 2. Prepare all reagents, working standards,samples and mix as directed in the respective sections. 3.Determine the number of wells to be used and put any remaining wells and the desiccant back into the pouch and seal the ziploc, store unused wells at 4 °C. 4. Add 50μL of standard or sample to the designated wells, Gentlely shake/tap the plate for 5 second to mix. 5. Add 50 μL of Mouse leptin Mix into all wells, including the blank wells, cover the plate with adhensive strip. Incubate for 2 hours at room temperature with gentle shaking. 6.Decant or aspirate contents of wells. Wash wells by filling with at least 300 μL/well prepared wash buffer followed by decanting/aspirating. Soak wells in wash buffer for 30 seconds to 1 minute for each wash. Repeat wash 4 times for a total of 5 washes. After the last wash, blot plate on absorbent paper to remove residual buffer. Thorough washing at this step is very important, complete removal of liquid is required for proper performance. 7.Pipette 100 μL of TMB Substrate Solution to each well. Incubate plate for 15 minutes at room temperature in the dark with gentle shaking. 8. Add 50μL of Stop Solution to each well, gently tap the plate to ensure thorough mixing. 9. Determine the optical density of each well within 15 minutes using a microplate reader set to 450nm. If wavelength correction is available, set to 570nm. Subtract readings at 570nm from the readings at 450nm. This subtraction will correct for optical imperfections in the plate.
Calculation of Results 1. Average the duplicate readings for each standard and sample and subtract the average zero standard optical density.2. Create a standard curve by reducing the data using computer software capable of generating a four parameter logistic (4-PL) curve fit. As an alternative, construct a standard curve by plotting the mean absorbance for each standard on the x-axis against the concentration on the y-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the target antigen concentration versus the log of the O.D. and the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data. 3. If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.
Assay Precision intra CV<10%, inter CV <15%
Restrictions For Research Use only

Handling

Product Details Leptin ELISA Kit Target details Application Details Images back to top
Buffer 0.05 % Proclin 300
Preservative ProClin
Precaution of Use The Stop Solution provided with this kit is an acid solution. Wear eye, hand, face and clothing protection when using this material.
Storage 4 °C/-20 °C

Images

Product Details Leptin ELISA Kit Target details Application Details Handling back to top
Supplier Images
ELISA image for Leptin ELISA Kit (LEP) (ABIN2506965) Leptin (LEP) ELISA Kit