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anti-Human Leptin Receptor antibody for Immunocytochemistry

Recommended Leptin Receptor Antibody (supplied by: Log in to see )

Antigen
Leptin Receptor (LEPR) Antibodies
  • cd295
  • CD295
  • db
  • diabetes
  • Fa
  • LEP-R
  • LEPR
  • Leprb
  • LEPRD
  • LEPROT
  • Modb1
  • OB-R
  • Ob-R
  • ob-rb
  • OB-RGRP
  • Ob-RM
  • obese-like
  • obl
  • obr
  • OBR
  • Obr
  • ObRb
  • Obrgrp
  • VPS55
Epitope
AA 310-538
23
22
22
17
14
13
6
5
4
4
4
4
3
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
180
61
53
13
8
2
1
1
1
1
1
Host
Rabbit
137
33
26
2
1
1
Clonality
Polyclonal
Conjugate
This Leptin Receptor antibody is un-conjugated
9
9
8
6
6
5
3
3
3
3
3
3
3
3
3
Application
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
129
105
48
34
31
7
6
6
2
1
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Catalog No. ABIN1859649
$ 347.60
Plus shipping costs $45.00

Similar anti-Leptin Receptor Antibodies

Application / Reactivity Human
ELISA 97 Antibodies
Enzyme Immunoassay (EIA) 7 Antibodies
Flow Cytometry (FACS) 6 Antibodies
Immunocytochemistry (ICC) 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 34 Antibodies
Immunohistochemistry (IHC) 46 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 5 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 30 Antibodies
Western Blotting (WB) 118 Antibodies

General

Antigen Leptin Receptor (LEPR) Antibodies
Epitope AA 310-538
(23), (22), (22), (17), (14), (13), (6), (5), (4), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(180), (61), (53), (13), (8), (2), (1), (1), (1), (1), (1)
Host Rabbit
(137), (33), (26), (2), (1), (1)
Clonality
Polyclonal
Conjugate This Leptin Receptor antibody is un-conjugated
(9), (9), (8), (6), (6), (5), (3), (3), (3), (3), (3), (3), (3), (3), (3)
Application Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
(129), (105), (48), (34), (31), (7), (6), (6), (2), (1)
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Product Details anti-Leptin Receptor Antibody

Target Details Leptin Receptor Application Details Handling Images
Sequence MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGS-G KRLDGPGIWS DWSTPRVFTT QDVIYFPPKI LTSVGSNVSF HCIYKKENKI VPSKEIVWWM NLAEKIPQSQ YDVVSDHVSK VTFFNLNETK PRGKFTYDAV YCCNEHECHH RYAELYVIDV NINISCETDG YLTKMTCRWS TSTIQSLAES TLQLRYHRSS LYCSDIPSIH PISEPKDCYL QSDGFYECIF QPIFLLSGYT MWIRINHSLG SLDSPPTCVL PDSVVKPL
Specificity It has been selected for its ability to recognize LEPR in immunohistochemical staining and Western blotting.
Purification Affinity Chromatography
Immunogen LEPR (AA 310-538)
Isotype IgG

Target Details Leptin Receptor

Product Details anti-Leptin Receptor Antibody Application Details Handling Images back to top
Antigen
Research Area Hormones, Receptors
Pathways JAK-STAT Signaling, AMPK Signaling

Application Details

Product Details anti-Leptin Receptor Antibody Target Details Leptin Receptor Handling Images back to top
Application Notes Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
Comment

Content: The quality control contains recombinant LEPR (Gly310~Leu538) disposed in loading buffer.
Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100 mM Tris(pH8.8), 2 % SDS, 200 mM NaCl, 50 % glycerol,BPB 0.01 % , NaN3 0.02 % .

Restrictions For Research Use only

Handling

Product Details anti-Leptin Receptor Antibody Target Details Leptin Receptor Application Details Images back to top
Format Liquid
Concentration 200 µg/mL
Buffer Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid repeated freeze/thaw cycles
Storage 4 °C
Storage Comment Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.
Expiry Date 12 months

Images

Product Details anti-Leptin Receptor Antibody Target Details Leptin Receptor Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-Leptin Receptor antibody (LEPR) (AA 310-538) (ABIN1859649) anti-Leptin Receptor (LEPR) (AA 310-538) antibody