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anti-Cow (Bovine) LIF antibody for Western Blotting

Recommended LIF Antibody (supplied by: Log in to see )

Antigen
Leukemia Inhibitory Factor (LIF) Antibodies
  • A230075M04Rik
  • AW061234
  • CDF
  • DIA
  • HILDA
  • LIF
  • MLPLI
Epitope
N-Term
15
14
8
7
5
5
3
3
3
3
3
2
2
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Pig (Porcine), Rat (Rattus)
113
63
23
5
5
4
3
Host
Rabbit
90
22
22
3
2
Clonality
Polyclonal
Conjugate
This LIF antibody is un-conjugated
4
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
110
41
35
29
26
6
5
4
3
3
2
1
1
Supplier
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Catalog No. ABIN2785833
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.13476625 ABIN2704959 WB Rabbit C-Term Log in to see Polyclonal
0.13476625 ABIN2706472 WB Rabbit Center Log in to see Polyclonal
0.0011760078 ABIN1862857 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG Log in to see Polyclonal
0.0011760078 ABIN2971965 WB Rabbit C-Term Log in to see Polyclonal

Top referenced anti-LIF antibody for Western Blotting

Similar anti-LIF Antibodies

Application / Reactivity Human Mouse (Murine) Cow (Bovine) Dog (Canine) Pig (Porcine) Rat (Rattus)
Western Blotting (WB) 92 Antibodies 55 Antibodies 5 Antibodies 2 Antibodies 5 Antibodies 21 Antibodies
Neutralization (Neut) 2 Antibodies
Immunoprecipitation (IP) 2 Antibodies 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 32 Antibodies 21 Antibodies 1 Antibodies 1 Antibodies 1 Antibodies
Immunohistochemistry (IHC) 26 Antibodies 3 Antibodies 3 Antibodies 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies

General

Antigen Leukemia Inhibitory Factor (LIF) Antibodies
Epitope N-Term
(15), (14), (8), (7), (5), (5), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Pig (Porcine), Rat (Rattus)
(113), (63), (23), (5), (5), (4), (3)
Host Rabbit
(90), (22), (22), (3), (2)
Clonality
Polyclonal
Conjugate This LIF antibody is un-conjugated
(4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Western Blotting (WB)
(110), (41), (35), (29), (26), (6), (5), (4), (3), (3), (2), (1), (1)
Pubmed 2 references available
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Product Details anti-LIF Antibody

Target Details LIF Application Details Handling References for anti-LIF Antibody (ABIN2785833) Images
Blocking Peptide Blocking peptide for this product available: ABIN979312
Sequence KVLAAGVVPL LLVLHWKHGA GSPLPITPVN ATCAIRHPCH NNLMNQIRSQ
Predicted Reactivity Bovine : 100 %, Dog : 100 %, Guinea pig : 100 %, Horse : 100 %, Human : 100 %, Mouse : 100 %, Rat : 100 %, Pig : 90 %
Characteristics This is a rabbit polyclonal antibody against LIF. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human LIF

Target Details LIF

Product Details anti-LIF Antibody Application Details Handling References for anti-LIF Antibody (ABIN2785833) Images back to top
Antigen
Alternative Name LIF (LIF Antibody Abstract)
Background LIF is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface.Leukaemia inhibitory factor is a cytokine that induces macrophage differentiation. Neurotransmitters and neuropeptides, well known for their role in the communication between neurons, are also capable of activating monocytes and macrophages and inducing chemotaxis in immune cells. LIF signals through different receptors and transcription factors. LIF in conjunction with BMP2 acts in synergy on primary fetal neural progenitor cells to induce astrocytes.
Molecular Weight 22 kDa
Gene ID 3976
NCBI Accession NP_002300, NM_002309
UniProt P15018
Pathways JAK-STAT Signaling

Application Details

Product Details anti-LIF Antibody Target Details LIF Handling References for anti-LIF Antibody (ABIN2785833) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 202 AA

Restrictions For Research Use only

Handling

Product Details anti-LIF Antibody Target Details LIF Application Details References for anti-LIF Antibody (ABIN2785833) Images back to top
Format Liquid
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C,-20 °C
Storage Comment For short term use, store at 2-8 °C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-LIF Antibody (ABIN2785833)

Product Details anti-LIF Antibody Target Details LIF Application Details Handling Images back to top
Product cited in:

Muntz, Malpass, McGonigle, Robertson, Weiden: "Phase 2 study of intraperitoneal topotecan as consolidation chemotherapy in ovarian and primary peritoneal carcinoma." in: Cancer, Vol. 113, Issue 3, pp. 490-6, 2008

Background publications

García-Tuñón, Ricote, Ruiz, Fraile, Paniagua, Royuela: "OSM, LIF, its receptors, and its relationship with the malignance in human breast carcinoma (in situ and in infiltrative)." in: Cancer investigation, Vol. 26, Issue 3, pp. 222-9, 2008

Images

Product Details anti-LIF Antibody Target Details LIF Application Details Handling References for anti-LIF Antibody (ABIN2785833) back to top
Supplier Images
Western Blotting (WB) image for anti-LIF antibody (Leukemia Inhibitory Factor) (N-Term) (ABIN2785833) WB Suggested Anti-LIF Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1...