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Dog (Canine) LIF ELISA Kit

Recommended LIF Product (supplied by: Log in to see )

Antigen
Leukemia Inhibitory Factor (LIF) ELISA Kits
  • CDF
  • DIA
  • HILDA
  • MLPLI
  • A230075M04Rik
  • AW061234
  • LIF
  • leukemia inhibitory factor
  • leukemia inhibitory factor (cholinergic differentiation factor)
  • leukemia inhibitory factor receptor
  • Lif
  • LIF
  • Lifr
Reactivity
Dog (Canine)
Alternatives
Kits with alternative reactivity to:
19
12
11
10
9
5
3
3
2
2
2
2
1
Method Type
Sandwich ELISA
Detection Range
31.25-2000 pg/mL
Minimum Detection Limit
31.25 pg/mL
Application
ELISA
Supplier
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Catalog No. ABIN2851328
$ 929.89
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
1 ABIN993476 Competition ELISA Serum, Plasma, Cell Culture Supernatant, Body Fluids, Tissue Homogenate 50-1000 pg/mL 50 pg/mL Log in to see
1 ABIN4970818 Sandwich ELISA Cell Culture Supernatant, Plasma, Tissue Homogenate, Serum, Cell Lysate, Biological Fluids 31.25-2000 pg/mL 31.25 pg/mL Log in to see
1 ABIN2089969 Sandwich ELISA Log in to see

Similar LIF ELISA Kits

Application / Reactivity Dog (Canine)
ELISA 4 ELISA Kits

General

Antigen Leukemia Inhibitory Factor (LIF) ELISA Kits
Reactivity Dog (Canine)
Kits with alternative reactivity to:
(19), (12), (11), (10), (9), (5), (3), (3), (2), (2), (2), (2), (1)
Method Type Sandwich ELISA
Detection Range 31.25-2000 pg/mL
Minimum Detection Limit 31.25 pg/mL
Application ELISA
Supplier Log in to see

Product Details LIF ELISA Kit

Target details Application Details Handling Images
Purpose The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of this index in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Sample Type Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids
Detection Method Colorimetric
Analytical Method Quantitative
Specificity This assay has high sensitivity and excellent specificity for detection of this index.
Cross-Reactivity (Details) No significant cross-reactivity or interference between this index and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between this index and all the analogues, therefore, cross reaction may still exist.
Sensitivity 14.7 pg/mL
Characteristics Canine Leukemia Inhibitory Factor (LIF) ELISA Kit
Components Assay plate: 1×
Standard: 2×
Sample Diluent : 1 × 20 mL
Assay Diluent A: 1 × 10 mL
Assay Diluent B: 1 × 10 mL
Detection Reagent A: 1 × 120 µL
Detection Reagent B: 1 × 120 µL
Wash Buffer(25 x concentrate): 1 × 30 mL
Substrate: 1 × 10 mL
Stop Solution: 1 × 10 mL
Plate sealer for 96 wells: 5×
Instruction: 1×
Material not included Microplate reader, Pipettes and pipette tips, EP tube, Deionized or distilled water.

Target details

Product Details LIF ELISA Kit Application Details Handling Images back to top
Antigen
Alternative Name LIF (LIF ELISA Kit Abstract)
Gene ID 403449
UniProt J9NRL6
Research Area Cancer
Pathways JAK-STAT Signaling, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Stem Cell Maintenance, Growth Factor Binding

Application Details

Product Details LIF ELISA Kit Target details Handling Images back to top
Sample Volume 100 μL
Assay Time 1 - 4.5 h
Plate Pre-coated,Strips (12 x 8)
Assay Precision Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level this index were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level this index were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Restrictions For Research Use only

Handling

Product Details LIF ELISA Kit Target details Application Details Images back to top
Precaution of Use The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
Handling Advice The user should calculate the possible amount of the samples used in the whole test. Please reserve sufficient samples in advance. 2. The kit should not be used beyond the expiration date on the kit label. 3. Do not mix or substitute reagents with those from other lots or sources. 4. If samples generate values higher than the highest standard, further dilute the samples with the Sample Diluent and repeat the assay. Any variation in standard diluent, operator, pipetting technique, washing technique,incubation time or temperature, and kit age can cause variation in binding.
Storage 4 °C
Storage Comment The Assay Plate, Standard, Detection Reagent A and Detection Reagent B should be stored at -20°C upon being received. After receiving the kit , Substrate should be always stored at 4°C.Other reagents are kept according to the labels on vials. But for long term storage, please keep the whole kit at -20°C. The unused strips should be kept in a sealed bag with the desiccant provided to minimize exposure to damp air. The test kit may be used throughout the expiration date of the kit (six months from the date of manufacture). Opened test kits will remain stable until the expiring date shown, provided it is stored as prescribed above.
Expiry Date 12 months