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anti-Mouse (Murine) SRC antibody for Flow Cytometry

Recommended SRC Antibody (supplied by: Log in to see )

Antigen
V-Src Sarcoma (Schmidt-Ruppin A-2) Viral Oncogene Homolog (Avian) (SRC) Antibodies
  • ASV
  • AW259666
  • c-src
  • c-SRC
  • c-Src
  • C-src1
  • CG7524
  • D-src
  • D-Src64B
  • Dmel\CG7524
  • DmHD-358
  • Dm SRC1
  • dsrc
  • DSrc
  • dSrc
  • Dsrc
  • DSRC64
  • DSrc64
  • Dsrc64
  • Dsrc64B
  • DSrc64B
  • HD-358
  • MRE5
  • p60-Src
  • PP60C-SCR
  • pp60c-src
  • SDR
  • src
  • Src
  • SRC1
  • src1
  • Src1
  • src64
  • Src64
  • SRC 64B
  • src64B
  • src64b
Epitope
pTyr418
76
34
29
27
25
22
19
15
14
13
11
8
6
6
5
4
4
4
4
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
404
186
150
21
9
7
7
4
4
2
2
2
2
1
1
1
1
1
1
1
1
Host
Mouse
358
69
1
Clonality
Monoclonal
Conjugate
This SRC antibody is conjugated to PE
19
15
15
14
13
13
3
3
2
2
2
2
2
2
2
1
1
1
Application
Flow Cytometry (FACS), Intracellular Staining (ICS)
349
200
158
37
33
24
23
20
13
7
5
5
2
1
Supplier
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Available images

Catalog No. ABIN2680936
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.30226976 ABIN2680940 FACS ICS PerCP-eFluor® 710 Mouse IgG2b kappa pTyr418 Log in to see SC1T2M3
0.30226976 ABIN2680942 FACS ICS EFluor 660 Mouse IgG2b kappa pTyr418 Log in to see SC1T2M3
0.0010482287 ABIN3060239 ICC FACS IF IP IHC WB Rabbit Log in to see Polyclonal

Similar anti-SRC Antibodies

Application / Reactivity Mouse (Murine) Human
Confocal Microscopy (CM) 2 Antibodies 2 Antibodies
ELISA 68 Antibodies 185 Antibodies
Flow Cytometry (FACS) 4 Antibodies 14 Antibodies
Immunochromatography (IC) 2 Antibodies 4 Antibodies
Immunocytochemistry (ICC) 4 Antibodies
Immunofluorescence (IF) 21 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 23 Antibodies
Immunohistochemistry (IHC) 88 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 16 Antibodies
Immunoprecipitation (IP) 7 Antibodies
Intracellular Staining (ICS) 6 Antibodies
Western Blotting (WB) 147 Antibodies
Dot Blot (DB) 20 Antibodies

General

Antigen V-Src Sarcoma (Schmidt-Ruppin A-2) Viral Oncogene Homolog (Avian) (SRC) Antibodies
Epitope pTyr418
(76), (34), (29), (27), (25), (22), (19), (15), (14), (13), (11), (8), (6), (6), (5), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(404), (186), (150), (21), (9), (7), (7), (4), (4), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Host Mouse
(358), (69), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This SRC antibody is conjugated to PE
(19), (15), (15), (14), (13), (13), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application Flow Cytometry (FACS), Intracellular Staining (ICS)
(349), (200), (158), (37), (33), (24), (23), (20), (13), (7), (5), (5), (2), (1)
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Product Details anti-SRC Antibody

Target Details SRC Application Details Handling Images
Specificity This SC1T2M3 monoclonal antibody recognizes human and mouse Src tyrosine kinase (also known as ASV, c-src, c-SRC, p60-Src, pp60c-src, Proto-oncogene c-Src, Proto-oncogene tyrosine-protein kinase Src, SRC1) when phosphorylated on tyrosine 418 (Y418). Autophosphorylation of Src at Y418 in the catalytic domain is required for full catalytic activity of this kinase. Src is a non-receptor tyrosine kinase involved in signal transduction in numerous biological systems and is activated following engagement of many different classes of cellular receptors including immune response receptors, integrins and other adhesion receptors, receptor protein tyrosine kinases, G protein-coupled receptors as well as cytokine receptors. Aberrant Src activity has been implicated in the development of numerous types of cancer. Due to the sequence homology surrounding Src Y418, this SC1T2M3 clone is predicted to cross-react with many Src family kinases including Src, Lck, Fyn, and Lyn.

Specificity of this SC1T2M3 clone was determined by ELISA, flow cytometry, and western blotting.
Characteristics This SC1T2M3 monoclonal antibody recognizes human and mouse Src tyrosine kinase (also known as ASV, c-src, c- SRC, p60-Src, pp60c-src, Proto-oncogene c-Src, Proto-oncogene tyrosine-protein kinase Src, SRC1) when phosphorylated on tyrosine 418 (Y418). Autophosphorylation of Src at Y418 in the catalytic domain is required for full catalytic activity of this kinase. Src is a non-receptor tyrosine kinase involved in signal transduction in numerous biological systems and is activated following engagement of many different classes of cellular receptors including immune response receptors, integrins and other adhesion receptors, receptor protein tyrosine kinases, G protein- coupled receptors as well as cytokine receptors. Aberrant Src activity has been implicated in the development of numerous types of cancer. Due to the sequence homology surrounding Src Y418, this SC1T2M3 clone is predicted to cross-react with many Src family kinases including Src, Lck, Fyn, and Lyn. Specificity of this SC1T2M3 clone was determined by ELISA, flow cytometry, and western blotting.
Synonyms: Synonyms: ASV, c-src, c-SRC, p60-Src, pp60c-src, Proto-oncogene c-Src, Proto-oncogene tyrosine-protein kinase Src, SRC1
Clone SC1T2M3
Isotype IgG2b kappa

Target Details SRC

Product Details anti-SRC Antibody Application Details Handling Images back to top
Antigen
Alternative Name Src (SRC Antibody Abstract)
Research Area Cancer, Transcription Factors, Tyrosine Kinases, Signaling
Pathways JAK-STAT Signaling, Neurotrophin Signaling Pathway

Application Details

Product Details anti-SRC Antibody Target Details SRC Handling Images back to top
Application Notes Applications reported: This SC1T2M3 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications tested: This SC1T2M3 antibody has been pre-titrated and tested by intracellular staining followed by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10 5 to 10 8 cells/test. Staining Protocol: All protocols work well for this monoclonal antibody. Use of Protocol A: Two-step protocol: intracellular (cytoplasmic) proteins allows for the greatest flexibility for detection of surface and intracellular (cytoplasmic) proteins. Use of Protocol B: One-step protocol: intracellular (nuclear) proteins is recommended for staining of transcription factors in conjunction with surface and phosphorylated intracellular (cytoplasmic) proteins. Protocol C: Two-step protocol: Fixation/Methanol allows for the Page 2 of 2 Anti-Human/Mouse phospho-Src (Y418) PE
Restrictions For Research Use only

Handling

Product Details anti-SRC Antibody Target Details SRC Application Details Images back to top
Buffer aqueous buffer, 0.09 % sodium azide, may contain carrier protein/stabilizer
Handling Advice Do not freeze. Light-sensitive material.
Storage 4 °C
Storage Comment Store at 2-8°C.

Images

Product Details anti-SRC Antibody Target Details SRC Application Details Handling back to top
Supplier Images
Flow Cytometry (FACS) image for anti-SRC antibody (V-Src Sarcoma (Schmidt-Ruppin A-2) Viral Oncogene Homolog (Avian)) (pTyr418) (PE) (ABIN2680936) anti-V-Src Sarcoma (Schmidt-Ruppin A-2) Viral Oncogene Homolog (Avian) (SRC) (pTyr418) antibody (PE)