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anti-Human STAT6 antibody for Enzyme Immunoassay

Recommended STAT6 Antibody (supplied by: Log in to see )

Antigen
Signal Transducer and Activator of Transcription 6, Interleukin-4 Induced (STAT6) Antibodies
  • D12S1644
  • IL-4-STAT
  • STAT6B
  • STAT6C
Epitope
pTyr641
80
38
22
13
13
11
10
10
6
5
5
5
5
4
4
4
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
354
115
97
12
8
7
6
4
2
Host
Mouse
248
133
3
1
Clonality
Monoclonal
Conjugate
This STAT6 antibody is un-conjugated
13
12
11
10
9
8
5
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
282
143
107
38
33
33
33
18
15
12
5
3
1
1
1
1
Supplier
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Catalog No. ABIN126901
$ 533.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00088188547 ABIN126902 EIA IHC (fro) WB Mouse IgG1 Log in to see 8C12
0.00088188547 ABIN1109154 EIA IF WB Mouse IgG1 Log in to see 7D3 3
0.00088188547 ABIN318132 EIA IHC (p) IP WB Rabbit pThr645 Log in to see Polyclonal

Top referenced anti-STAT6 antibody for Enzyme Immunoassay

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Application / Reactivity Human
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ELISA (Capture) 1 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies
Flow Cytometry (FACS) 18 Antibodies
Immunoassay (IA) 1 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 10 Antibodies
Immunofluorescence (IF) 33 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies
Immunohistochemistry (IHC) 109 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 4 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 35 Antibodies
Immunoprecipitation (IP) 34 Antibodies
Intracellular Staining (ICS) 12 Antibodies
Western Blotting (WB) 271 Antibodies

General

Antigen Signal Transducer and Activator of Transcription 6, Interleukin-4 Induced (STAT6) Antibodies
Epitope pTyr641
(80), (38), (22), (13), (13), (11), (10), (10), (6), (5), (5), (5), (5), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(354), (115), (97), (12), (8), (7), (6), (4), (2)
Host Mouse
(248), (133), (3), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This STAT6 antibody is un-conjugated
(13), (12), (11), (10), (9), (8), (5), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (1), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
(282), (143), (107), (38), (33), (33), (33), (18), (15), (12), (5), (3), (1), (1), (1), (1)
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Product Details anti-STAT6 Antibody

Target Details STAT6 Application Details Handling Images
Specificity This antibody specifically recognizes activated STAT6 phosphorylated at Tyr641 at 100 kDa. It does not cross-react with non-phosphorylated form of STAT6 nor with unrelated phosphorylation sites.
Characteristics Synonyms: STAT-6, IL-4 Stat, IL-4-STAT, Signal transducer and activator of transcription 6
Purification Size Exclusion Chromatography
Components incl. positive Control
Immunogen Synthetic phosphopeptide conjuagted to KLH
Clone 1-60E-013
Isotype IgG1

Target Details STAT6

Product Details anti-STAT6 Antibody Application Details Handling Images back to top
Antigen
Alternative Name STAT6 (STAT6 Antibody Abstract)
Background The STAT proteins serve as both cytoplasmic signal transducers and nuclear activators of transcription. STATs are mediators involved in cytokine signalling. In response to a specific cytokine signal, STAT proteins are phosphorylated on conserved tyrosine residues. Phosphorylated STAT proteins dimerize via their SH2 domains and move to the nucleus. The STAT dimers bind to specific DNA elements resulting in transcriptional regulation of downstream target genes. STAT6 is activated primarily by IL-4 and IL-13. Upon activation, STAT6 is phosphorylated at tyrosine 641 by Janus Kinase (JAK). Phosphorylated STAT6 forms head-to-tail heterodimers and translocates to the nucleus where it paricipates in transcriptional control.Synonyms: IL-4 Stat, Signal transducer and activator of transcription 6
Gene ID 6778
UniProt P42226
Research Area Chromatin and Nuclear Signaling, Signaling, Receptors, Inflammation
Pathways JAK-STAT Signaling

Application Details

Product Details anti-STAT6 Antibody Target Details STAT6 Handling Images back to top
Application Notes Western Blot: 1 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. ELISA: 0.05 μg/mL. Immunohistochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-STAT6 Antibody Target Details STAT6 Application Details Images back to top
Reconstitution Restore with 1 mL H2O (15 min, RT).
Buffer 1 mL2 x PBS containing 0.09 % Sodium Azide, PEG and Sucrose
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C/-80 °C
Storage Comment Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
Shelf life: one year from despatch.
Expiry Date 12 months

Images

Product Details anti-STAT6 Antibody Target Details STAT6 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-STAT6 antibody (Signal Transducer and Activator of Transcription 6, Interleukin-4 Induced) (pTyr641) (ABIN126901) anti-Signal Transducer and Activator of Transcription 6, Interleukin-4 Induced (STAT6) (pTyr641) antibody