anti-Human STAT6 antibody for Intracellular Staining

Recommended STAT6 Antibody (supplied by: Log in to see )

Antigen
Signal Transducer and Activator of Transcription 6, Interleukin-4 Induced (STAT6) Antibodies
  • D12S1644
  • IL-4-STAT
  • STAT6B
  • STAT6C
  • signal transducer and activator of transcription 6, interleukin-4 induced
  • signal transducer and activator of transcription 6
  • signal transducer and activator of transcription 6-like
  • STAT6
  • Stat6
  • LOC100859543
Epitope
pTyr641
91
41
22
19
17
15
15
11
8
6
5
5
5
5
5
4
4
4
4
4
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
441
189
133
24
20
17
15
14
9
6
3
2
1
1
1
Host
Mouse
288
178
6
2
1
Clonality
Monoclonal
Conjugate
This STAT6 antibody is un-conjugated
17
17
15
14
13
10
8
8
6
6
6
6
6
5
5
5
5
5
5
5
4
3
3
3
3
3
3
3
2
Application
Immunofluorescence (IF), Intracellular Staining (ICS), Western Blotting (WB)
340
148
135
67
54
50
46
39
35
10
5
4
4
4
3
1
1
1
1
1
1
Options
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Catalog No. ABIN968593
Contact our Customer Service for availability and price in your country.
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1176939 ICS PE Mouse IgG1 C-Term Log in to see 23-Stat6 1
1 ABIN1176940 ICS Alexa Fluor 647 Mouse IgG1 C-Term Log in to see 23-Stat6 1
1 ABIN1177211 ICS Alexa Fluor 647 Mouse IgG2a pTyr641 Log in to see 18-P 6
1 ABIN1177210 ICS PE Mouse IgG2a pTyr641 Log in to see 18-P 6
1 ABIN1177209 ICS Alexa Fluor 488 Mouse IgG2a pTyr641 Log in to see 18-P 1
1 ABIN1177207 ICS PE Mouse IgG2a pTyr641 Log in to see 18-P
1 ABIN1177208 ICS Alexa Fluor 647 Mouse IgG2a pTyr641 Log in to see 18-P

General

Antigen Signal Transducer and Activator of Transcription 6, Interleukin-4 Induced (STAT6) Antibodies
Epitope pTyr641
(91), (41), (22), (19), (17), (15), (15), (11), (8), (6), (5), (5), (5), (5), (5), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(441), (189), (133), (24), (20), (17), (15), (14), (9), (6), (3), (2), (1), (1), (1)
Host Mouse
(288), (178), (6), (2), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This STAT6 antibody is un-conjugated
(17), (17), (15), (14), (13), (10), (8), (8), (6), (6), (6), (6), (6), (5), (5), (5), (5), (5), (5), (5), (4), (3), (3), (3), (3), (3), (3), (3), (2)
Application Immunofluorescence (IF), Intracellular Staining (ICS), Western Blotting (WB)
(340), (148), (135), (67), (54), (50), (46), (39), (35), (10), (5), (4), (4), (4), (3), (1), (1), (1), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-STAT6 Antibody

Target Details STAT6 Application Details Handling References for anti-STAT6 antibody (ABIN968593) Images
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Phosphorylated Human Stat6 (pY641)
Clone 18-P
Isotype IgG2a

Target Details STAT6

Product Details anti-STAT6 Antibody Application Details Handling References for anti-STAT6 antibody (ABIN968593) Images back to top
Antigen
Alternative Name Stat6 (STAT6 Antibody Abstract)
Background Interleukin-4 (IL-4), a major immunoregulatory cytokine, is secreted by activated T lymphocytes, basophils, and mast cells and plays an important role in modulating T helper cell lineage development. It induces specific gene expression via the tyrosine phosphorylation of Stat6 at tyrosine 641 (Y641). Stat6, a member of the signal transducers and activators of transcription protein family, mediates signals for IL-4 and, possibly, IL-13. While Stat6 is widely expressed in human tissues, it exhibits elevated expression in peripheral blood lymphocytes, colon, intestine, ovary, prostate, thymus, spleen, kidney, liver, lung, and placenta. Following cytokine receptor ligation, Jak kinases are activated and phosphorylate the cytoplasmic tails of the oligomerized receptors. The SH3:SH2 domain of Stat6 associates with tyrosine-phosphorylated IL-4 receptor and the proximal Jak kinase phosphorylates Stat6 at Y641 on the C-terminal side of the SH2 domain. Stat6 is then released from the receptor, dimerizes, and is thought to contact the basal transcription machinery by binding to p300/CBP. Thus, Stat6 mediates the IL-4 signal and is essential for the proper development of adaptive immunity.
Molecular Weight 100 kDa
Research Area Signaling, Receptors, Inflammation
Pathways JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response

Application Details

Product Details anti-STAT6 Antibody Target Details STAT6 Handling References for anti-STAT6 antibody (ABIN968593) Images back to top
Restrictions For Research Use only

Handling

Product Details anti-STAT6 Antibody Target Details STAT6 Application Details References for anti-STAT6 antibody (ABIN968593) Images back to top
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20° C.

References for anti-STAT6 antibody (ABIN968593)

Product Details anti-STAT6 Antibody Target Details STAT6 Application Details Handling Images back to top
Product cited in:

Mikita, Campbell, Wu, Williamson, Schindler: "Requirements for interleukin-4-induced gene expression and functional characterization of Stat6." in: Molecular and cellular biology, Vol. 16, Issue 10, pp. 5811-20, 1996

Hou, Schindler, Henzel, Ho, Brasseur, McKnight: "An interleukin-4-induced transcription factor: IL-4 Stat." in: Science (New York, N.Y.), Vol. 265, Issue 5179, pp. 1701-6, 1994

Background publications

Quelle, Shimoda, Thierfelder, Fischer, Kim, Ruben, Cleveland, Pierce, Keegan, Nelms: "Cloning of murine Stat6 and human Stat6, Stat proteins that are tyrosine phosphorylated in responses to IL-4 and IL-3 but are not required for mitogenesis." in: Molecular and cellular biology, Vol. 15, Issue 6, pp. 3336-43, 1995

Images

Product Details anti-STAT6 Antibody Target Details STAT6 Application Details Handling References for anti-STAT6 antibody (ABIN968593) back to top
Supplier Images
Immunofluorescence (IF) image for anti-Signal Transducer and Activator of Transcription 6, Interleukin-4 Induced (STAT6) (pTyr641) antibody (ABIN968593) Immunofluorescent labelling of phosphorylated Stat6 in endothelial cells exposed to 1...
 image for anti-Signal Transducer and Activator of Transcription 6, Interleukin-4 Induced (STAT6) (pTyr641) antibody (ABIN968593) Human Endothelial cells were either untreated (left column) or treated with (right co...