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|Application / Reactivity||Human|
|Biochemical Assay (BCA)||1 Antibodies|
|ELISA (Capture)||1 Antibodies|
|Enzyme Immunoassay (EIA)||4 Antibodies|
|Flow Cytometry (FACS)||18 Antibodies|
|Immunoassay (IA)||1 Antibodies|
|Immunochromatography (IC)||1 Antibodies|
|Immunocytochemistry (ICC)||10 Antibodies|
|Immunofluorescence (IF)||33 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||22 Antibodies|
|Immunohistochemistry (IHC)||109 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||4 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||35 Antibodies|
|Immunoprecipitation (IP)||33 Antibodies|
|Intracellular Staining (ICS)||12 Antibodies|
|Western Blotting (WB)||210 Antibodies|
|Antigen||Signal Transducer and Activator of Transcription 6, Interleukin-4 Induced (STAT6) Antibodies|
|Conjugate||This STAT6 antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|4 references available|
|Supplier||Log in to see|
Product Details anti-STAT6 AntibodyTarget Details STAT6 Application Details Handling References for anti-STAT6 Antibody (ABIN196700) Images
|Specificity||The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. STAT6 (phospho-Tyr641) antibody detects endogenous levels of STAT6 only when phosphorylated at tyrosine 641.|
|Immunogen||The antiserum was produced against synthesized phosphopeptide derived from human STAT6 around the phosphorylation site of Tyrosine 641 (R-G-YP-V-P).|
Target Details STAT6Product Details anti-STAT6 Antibody Application Details Handling References for anti-STAT6 Antibody (ABIN196700) Images back to top
|Alternative Name||STAT6 (STAT6 Antibody Abstract)|
|Background||Synonyms: IL-4 Stat, Signal transducer and activator of transcription 6|
|Research Area||Chromatin and Nuclear Signaling, Signaling, Receptors, Inflammation|
Application DetailsProduct Details anti-STAT6 Antibody Target Details STAT6 Handling References for anti-STAT6 Antibody (ABIN196700) Images back to top
Suitable for use in Western blot (1: 500approx. 1: 1000) and Immunohistochemistry (1: 50approx. 1: 100).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-STAT6 Antibody Target Details STAT6 Application Details References for anti-STAT6 Antibody (ABIN196700) Images back to top
|Buffer||Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % glycerol.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage Comment||Store the antibody (in aliquots) at -20 °C.|
References for anti-STAT6 Antibody (ABIN196700)Product Details anti-STAT6 Antibody Target Details STAT6 Application Details Handling Images back to top
Nelms, Keegan, Zamorano et al.: "The IL-4 receptor: signaling mechanisms and biologic functions." in: Annual review of immunology, Vol. 17, pp. 701-38, 1999 (PubMed).
Malabarba, Rui, Deutsch et al.: "Interleukin-13 is a potent activator of JAK3 and STAT6 in cells expressing interleukin-2 receptor-gamma and interleukin-4 receptor-alpha." in: The Biochemical journal, Vol. 319 ( Pt 3), pp. 865-72, 1996 (PubMed).
Hou, Schindler, Henzel et al.: "An interleukin-4-induced transcription factor: IL-4 Stat." in: Science (New York, N.Y.), Vol. 265, Issue 5179, pp. 1701-6, 1994 (PubMed).
Quelle, Shimoda, Thierfelder et al.: "Cloning of murine Stat6 and human Stat6, Stat proteins that are tyrosine phosphorylated in responses to IL-4 and IL-3 but are not required for mitogenesis." in: Molecular and cellular biology, Vol. 15, Issue 6, pp. 3336-43, 1995 (PubMed).
ImagesProduct Details anti-STAT6 Antibody Target Details STAT6 Application Details Handling References for anti-STAT6 Antibody (ABIN196700) back to top