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anti-Mouse (Murine) STAT6 antibody for Flow Cytometry

Recommended STAT6 Antibody (supplied by: Log in to see )

Antigen
Signal Transducer and Activator of Transcription 6, Interleukin-4 Induced (STAT6) Antibodies
  • D12S1644
  • IL-4-STAT
  • STAT6B
  • STAT6C
Epitope
pTyr641
80
38
23
13
13
11
10
10
7
6
5
5
5
5
4
4
4
3
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
388
140
102
12
8
7
6
4
2
1
Host
Mouse
256
159
3
1
Clonality
Monoclonal
Conjugate
This STAT6 antibody is conjugated to PE
13
12
12
12
10
9
5
5
5
4
4
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
2
1
1
1
Application
Flow Cytometry (FACS), Intracellular Staining (ICS)
309
144
113
53
46
38
33
24
17
14
6
4
2
1
1
1
1
1
Supplier
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Available images

Catalog No. ABIN2681001
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.30226976 ABIN2681003 FACS ICS APC Mouse IgG2a kappa pTyr641 Log in to see CHI2S4N
0.30226976 ABIN2681005 FACS ICS PerCP-eFluor® 710 Mouse IgG2a kappa pTyr641 Log in to see CHI2S4N
0.30226976 ABIN2681007 FACS ICS PE-eFluor® 610 Mouse IgG2a kappa pTyr641 Log in to see CHI2S4N
0.0010482287 ABIN336272 FACS IHC WB Mouse IgG AA 342-640 Log in to see 90509

Similar anti-STAT6 Antibodies

Application / Reactivity Mouse (Murine) Human
Biochemical Assay (BCA) 1 Antibodies 1 Antibodies
ELISA 24 Antibodies 138 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies 4 Antibodies
Flow Cytometry (FACS) 5 Antibodies
Immunocytochemistry (ICC) 8 Antibodies
Immunofluorescence (IF) 14 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 33 Antibodies
Immunohistochemistry (IHC) 33 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 25 Antibodies
Immunoprecipitation (IP) 34 Antibodies
Intracellular Staining (ICS) 9 Antibodies
Western Blotting (WB) 96 Antibodies
Chromatin Immunoprecipitation (ChIP) 1 Antibodies
ELISA (Capture) 1 Antibodies

General

Antigen Signal Transducer and Activator of Transcription 6, Interleukin-4 Induced (STAT6) Antibodies
Epitope pTyr641
(80), (38), (23), (13), (13), (11), (10), (10), (7), (6), (5), (5), (5), (5), (4), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(388), (140), (102), (12), (8), (7), (6), (4), (2), (1)
Host Mouse
(256), (159), (3), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This STAT6 antibody is conjugated to PE
(13), (12), (12), (12), (10), (9), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (2), (1), (1), (1)
Application Flow Cytometry (FACS), Intracellular Staining (ICS)
(309), (144), (113), (53), (46), (38), (33), (24), (17), (14), (6), (4), (2), (1), (1), (1), (1), (1)
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Product Details anti-STAT6 Antibody

Target Details STAT6 Application Details Handling Images
Specificity This CHI2S4N monoclonal antibody recognizes human and mouse signal transducer and activator of transcription 6 (STAT6) when phosphorylated on tyrosine 641. Following their phosphorylation by JAKs, STAT proteins translocate to the nucleus where they bind to DNA and regulate transcription of specific genes in a cell type- and cytokine-specific manner. In response to IL-4, STAT6 is phosphorylated on tyrosine 641 by JAK1 and JAK3. STAT6 signaling downstream of the IL-4 receptor promotes T cell growth and B cell production of IgE.

Specificity of this CHI2S4N clone was determined by ELISA and flow cytometry.
Characteristics This CHI2S4N monoclonal antibody recognizes human and mouse signal transducer and activator of transcription 6 (STAT6) when phosphorylated on tyrosine 641. Following their phosphorylation by JAKs, STAT proteins translocate to the nucleus where they bind to DNA and regulate transcription of specific genes in a cell type- and cytokine- specific manner. In response to IL-4, STAT6 is phosphorylated on tyrosine 641 by JAK1 and JAK3. STAT6 signaling downstream of the IL-4 receptor promotes T cell growth and B cell production of IgE. Specificity of this CHI2S4N clone was determined by ELISA and flow cytometry.
Synonyms: Synonyms: signal transducer and activator of transcription 6, pSTAT6
Clone CHI2S4N
Isotype IgG2a kappa

Target Details STAT6

Product Details anti-STAT6 Antibody Application Details Handling Images back to top
Antigen
Alternative Name STAT6 (STAT6 Antibody Abstract)
Research Area Chromatin and Nuclear Signaling, Signaling, Receptors, Inflammation
Pathways JAK-STAT Signaling

Application Details

Product Details anti-STAT6 Antibody Target Details STAT6 Handling Images back to top
Application Notes Applications reported: This CHI2S4N antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications tested: This CHI2S4N antibody has been pre-titrated and tested by intracellular staining followed by flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at 5 μL (0.125 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10 5 to 10 8 cells/test. Staining Protocol: We recommend using Protocol C: Two-step protocol: Fixation/Methanol. Protocol A: Two- step protocol: intracellular (cytoplasmic) proteins and Protocol B: One-step protocol: intracellular (nuclear) proteins cannot be used. All Protocols can be found in the Flow Cytometry Protocols: "Staining Intracellular Antigens for Flow Cytometry Protocol" located in the Best Protocols Section under the Resources tab online.
Restrictions For Research Use only

Handling

Product Details anti-STAT6 Antibody Target Details STAT6 Application Details Images back to top
Buffer aqueous buffer, 0.09 % sodium azide, may contain carrier protein/stabilizer
Handling Advice Do not freeze. Light-sensitive material.
Storage 4 °C
Storage Comment Store at 2-8°C.

Images

Product Details anti-STAT6 Antibody Target Details STAT6 Application Details Handling back to top
Supplier Images
Flow Cytometry (FACS) image for anti-STAT6 antibody (Signal Transducer and Activator of Transcription 6, Interleukin-4 Induced) (pTyr641) (PE) (ABIN2681001) anti-Signal Transducer and Activator of Transcription 6, Interleukin-4 Induced (STAT6) (pTyr641) antibody (PE)