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|Application / Reactivity||Mouse (Murine)||Human|
|Biochemical Assay (BCA)||1 Antibodies||1 Antibodies|
|ELISA||23 Antibodies||77 Antibodies|
|Enzyme Immunoassay (EIA)||2 Antibodies||4 Antibodies|
|Flow Cytometry (FACS)||5 Antibodies||18 Antibodies|
|Immunocytochemistry (ICC)||3 Antibodies|
|Immunofluorescence (IF)||9 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||33 Antibodies|
|Immunohistochemistry (IHC)||29 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||1 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||19 Antibodies|
|Immunoprecipitation (IP)||14 Antibodies|
|Intracellular Staining (ICS)||9 Antibodies|
|Western Blotting (WB)||70 Antibodies|
|ELISA (Capture)||1 Antibodies|
|Antigen||Signal Transducer and Activator of Transcription 6, Interleukin-4 Induced (STAT6) Antibodies|
|Reactivity||Human, Mouse (Murine) Alternatives|
|Conjugate||This STAT6 antibody is conjugated to PerCP-eFluor® 710 Alternatives|
Flow Cytometry (FACS), Intracellular Staining (ICS)
|Supplier||Log in to see|
Product Details anti-STAT6 AntibodyTarget Details STAT6 Application Details Handling Images
This CHI2S4N monoclonal antibody recognizes human and mouse signal transducer and activator of transcription 6 (STAT6) when phosphorylated on tyrosine 641. Following their phosphorylation by JAKs, STAT proteins translocate to the nucleus where they bind to DNA and regulate transcription of specific genes in a cell type- and cytokine-specific manner. In response to IL-4, STAT6 is phosphorylated on tyrosine 641 by JAK1 and JAK3. STAT6 signaling downstream of the IL-4 receptor promotes T cell growth and B cell production of IgE.
Specificity of this CHI2S4N clone was determined by ELISA and flow cytometry.
This CHI2S4N monoclonal antibody recognizes human and mouse signal transducer and activator of transcription 6 (STAT6) when phosphorylated on tyrosine 641. Following their phosphorylation by JAKs, STAT proteins translocate to the nucleus where they bind to DNA and regulate transcription of specific genes in a cell type- and cytokine- specific manner. In response to IL-4, STAT6 is phosphorylated on tyrosine 641 by JAK1 and JAK3. STAT6 signaling downstream of the IL-4 receptor promotes T cell growth and B cell production of IgE. Specificity of this CHI2S4N clone was determined by ELISA and flow cytometry.
Synonyms: Synonyms: signal transducer and activator of transcription 6, pSTAT6
Target Details STAT6Product Details anti-STAT6 Antibody Application Details Handling Images back to top
|Alternative Name||STAT6 (STAT6 Antibody Abstract)|
|Research Area||Chromatin and Nuclear Signaling, Signaling, Receptors, Inflammation|
Application DetailsProduct Details anti-STAT6 Antibody Target Details STAT6 Handling Images back to top
Applications reported: This CHI2S4N antibody has been reported for use in intracellular staining followed by flow cytometric analysis.
Applications tested: This CHI2S4N antibody has been pre-titrated and tested by intracellular staining followed by flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at 5 μL (0.03 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10 5 to 10 8 cells/test. Staining Protocol: We recommend using Protocol C: Two-step protocol: Fixation/Methanol. Protocol A: Two- step protocol: intracellular (cytoplasmic) proteins and Protocol B: One-step protocol: intracellular (nuclear) proteins cannot be used. All Protocols can be found in the Flow Cytometry Protocols: "Staining Intracellular Antigens for Flow Cytometry Protocol" located in the Best Protocols Section under the Resources tab online. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm), it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an Page 2 of 2 Anti-Human/Mouse phospho-STAT6 (Y641) PerCP-eFluor® 710
|Restrictions||For Research Use only|
HandlingProduct Details anti-STAT6 Antibody Target Details STAT6 Application Details Images back to top
|Buffer||aqueous buffer, 0.09 % sodium azide, may contain carrier protein/stabilizer|
|Handling Advice||Do not freeze. Light-sensitive material. This tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage, handling & experimental procedures.|
|Storage Comment||Store at 2-8°C.|
ImagesProduct Details anti-STAT6 Antibody Target Details STAT6 Application Details Handling back to top