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anti-Mouse (Murine) STAT6 antibody for Intracellular Staining

Recommended STAT6 Antibody (supplied by: Log in to see )

Antigen
Signal Transducer and Activator of Transcription 6, Interleukin-4 Induced (STAT6) Antibodies
  • D12S1644
  • IL-4-STAT
  • STAT6B
  • STAT6C
Epitope
pTyr641
80
38
22
13
13
11
10
10
6
5
5
5
5
4
4
4
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Mouse (Murine)
294
113
97
12
8
7
6
4
2
Host
Mouse
248
72
3
1
Clonality
Monoclonal
Conjugate
This STAT6 antibody is conjugated to Alexa Fluor 647
10
9
8
6
5
5
5
3
3
3
3
3
3
3
2
1
1
Application
Intracellular Staining (ICS)
222
107
83
38
33
33
32
18
14
12
6
4
1
1
1
1
Supplier
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Catalog No. ABIN1177230
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00087403273 ABIN1177232 ICS Alexa Fluor 488 Mouse IgG1, kappa pTyr641 Log in to see J71-773-58-11 2
0.00087403273 ABIN1177231 ICS PE Mouse IgG1, kappa pTyr641 Log in to see J71-773-58-11 1
0.00087403273 ABIN1176939 ICS PE Mouse IgG1 C-Term Log in to see 23-Stat6 1
0.00087403273 ABIN1176940 ICS Alexa Fluor 647 Mouse IgG1 C-Term Log in to see 23-Stat6 1
0.00087403273 ABIN2681001 FACS ICS PE Mouse IgG2a kappa pTyr641 Log in to see CHI2S4N
0.00087403273 ABIN2681003 FACS ICS APC Mouse IgG2a kappa pTyr641 Log in to see CHI2S4N
0.00087403273 ABIN2681005 FACS ICS PerCP-eFluor® 710 Mouse IgG2a kappa pTyr641 Log in to see CHI2S4N
0.00087403273 ABIN2681007 FACS ICS PE-eFluor® 610 Mouse IgG2a kappa pTyr641 Log in to see CHI2S4N

Top referenced anti-STAT6 antibody for Intracellular Staining

Similar anti-STAT6 Antibodies

Application / Reactivity Mouse (Murine)
Biochemical Assay (BCA) 1 Antibodies
ELISA 23 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Flow Cytometry (FACS) 5 Antibodies
Immunocytochemistry (ICC) 3 Antibodies
Immunofluorescence (IF) 9 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 33 Antibodies
Immunohistochemistry (IHC) 29 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 19 Antibodies
Immunoprecipitation (IP) 14 Antibodies
Intracellular Staining (ICS) 9 Antibodies
Western Blotting (WB) 70 Antibodies

General

Antigen Signal Transducer and Activator of Transcription 6, Interleukin-4 Induced (STAT6) Antibodies
Epitope pTyr641
(80), (38), (22), (13), (13), (11), (10), (10), (6), (5), (5), (5), (5), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Mouse (Murine)
(294), (113), (97), (12), (8), (7), (6), (4), (2)
Host Mouse
(248), (72), (3), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This STAT6 antibody is conjugated to Alexa Fluor 647
(10), (9), (8), (6), (5), (5), (5), (3), (3), (3), (3), (3), (3), (3), (2), (1), (1)
Application Intracellular Staining (ICS)
(222), (107), (83), (38), (33), (33), (32), (18), (14), (12), (6), (4), (1), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-STAT6 Antibody

Target Details STAT6 Application Details Handling References for anti-STAT6 Antibody (ABIN1177230) Images
Brand BD Phosflow™
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Phosphorylated Mouse STAT6 (Y641) Peptide
Clone J71-773-58-11
Isotype IgG1, kappa

Target Details STAT6

Product Details anti-STAT6 Antibody Application Details Handling References for anti-STAT6 Antibody (ABIN1177230) Images back to top
Antigen
Alternative Name Stat6 (STAT6 Antibody Abstract)
Background STATs (signal transducers and activators of transcription) are critical mediators of the biologic activity of cytokines including Interleukins (IL) 2-5, IL-7, IL-15, GM-CSF, erythropoietin and growth hormone. Ligand-receptor interaction leads to activation of constitutively associated JAK family kinases and subsequent recruitment/activation of STATs by tyrosine phosphorylation. Active STATs then move to the nucleus to promote transcription of cytokine-inducible genes. Seven STAT proteins have been cloned, each of which is differentially expressed and/or activated in a cytokine-specific and cell type-specific manner. Stat6 plays an important role in signaling pathways that lead to the differentiation of T helper type 2 (Th2) cells from uncommitted CD4 T cell precursors. Moreover, IL-4, secreted by activated T lymphocytes, basophils, and mast cells, induces specific gene expression via the induction of tyrosine phosphorylation of Stat6 at tyrosine 641 (Y641). The SH3:SH2 domain of Stat6 associates with tyrosine-phosphorylated IL-4 receptor and the proximal Jak kinase phosphorylates Stat6 at Y641 on the C-terminal side of the SH2 domain. Stat6 is then released from the receptor, dimerizes, and is thought to contact the basal transcription machinery by binding to p300/CBP. While Stat6 is widely expressed in human tissues, it exhibits elevated expression in peripheral blood lymphocytes, colon, intestine, ovary, prostate, thymus, spleen, kidney, liver, lung, and placenta. The J71-773.58.11 antibody recognizes mouse Stat6 phosphorylated at Y641.
Research Area Inflammation
Pathways JAK-STAT Signaling

Application Details

Product Details anti-STAT6 Antibody Target Details STAT6 Handling References for anti-STAT6 Antibody (ABIN1177230) Images back to top
Application Notes Note: For flow cytometric analysis of human cells, Alexa Fluor® 647 Mouse Anti-Hunan Stat6 (pY641) mAb (clone 18) (Cat. No. 612601) is recommended.
Sample Volume 20 μL
Restrictions For Research Use only

Handling

Product Details anti-STAT6 Antibody Target Details STAT6 Application Details References for anti-STAT6 Antibody (ABIN1177230) Images back to top
Format Liquid
Buffer Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.

References for anti-STAT6 Antibody (ABIN1177230)

Product Details anti-STAT6 Antibody Target Details STAT6 Application Details Handling Images back to top
Product cited in:

Daley, Brancato, Thomay, Reichner, Albina: "The phenotype of murine wound macrophages." in: Journal of leukocyte biology, Vol. 87, Issue 1, pp. 59-67, 2010

Hale, Krutzik, Samra, Crane, Nolan: "Stage dependent aberrant regulation of cytokine-STAT signaling in murine systemic lupus erythematosus." in: PLoS ONE, Vol. 4, Issue 8, pp. e6756, 2009

Mikita, Campbell, Wu, Williamson, Schindler: "Requirements for interleukin-4-induced gene expression and functional characterization of Stat6." in: Molecular and cellular biology, Vol. 16, Issue 10, pp. 5811-20, 1996

Quelle, Shimoda, Thierfelder, Fischer, Kim, Ruben, Cleveland, Pierce, Keegan, Nelms: "Cloning of murine Stat6 and human Stat6, Stat proteins that are tyrosine phosphorylated in responses to IL-4 and IL-3 but are not required for mitogenesis." in: Molecular and cellular biology, Vol. 15, Issue 6, pp. 3336-43, 1995

Images

Product Details anti-STAT6 Antibody Target Details STAT6 Application Details Handling References for anti-STAT6 Antibody (ABIN1177230) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-STAT6 antibody (Signal Transducer and Activator of Transcription 6, Interleukin-4 Induced) (pTyr641) (Alexa Fluor 647) (ABIN1177230) anti-Signal Transducer and Activator of Transcription 6, Interleukin-4 Induced (STAT6) (pTyr641) antibody (Alexa Fluor 647)