anti-Human Thrombopoietin antibody for Functional Studies

Recommended Thrombopoietin Antibody (supplied by: Log in to see )

Antigen
Thrombopoietin (THPO) Antibodies
  • tpo
  • LOC100231762
  • TPO
  • MGDF
  • MKCSF
  • ML
  • MPLLG
  • THCYT1
  • Mgdf
  • Ml
  • Mpllg
  • Tpo
  • Tpo1
  • Tpo2
  • Tpo3
  • Tpo4
  • thrombopoietin
  • thrombopoietin-like
  • THPO
  • thpo
  • LOC100231762
  • Thpo
  • LOC100620258
  • LOC100357079
  • LOC100855822
Reactivity
Human
149
44
11
4
2
1
1
1
1
1
1
Host
Goat
89
59
30
15
Clonality
Polyclonal
Conjugate
This Thrombopoietin antibody is un-conjugated
13
8
5
3
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
Application
Enzyme Immunoassay (EIA), Functional Studies (Func), Western Blotting (WB)
152
112
21
20
11
11
10
6
6
5
3
3
2
2
1
1
1
1
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Catalog No. ABIN116255
$ 265.65
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN116256 EIA Func WB Goat Log in to see Polyclonal

General

Antigen Thrombopoietin (THPO) Antibodies
Reactivity Human
(149), (44), (11), (4), (2), (1), (1), (1), (1), (1), (1)
Host Goat
(89), (59), (30), (15)
Clonality
Polyclonal
Conjugate This Thrombopoietin antibody is un-conjugated
(13), (8), (5), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Application Enzyme Immunoassay (EIA), Functional Studies (Func), Western Blotting (WB)
(152), (112), (21), (20), (11), (11), (10), (6), (6), (5), (3), (3), (2), (2), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-Thrombopoietin Antibody

Target Details Thrombopoietin Application Details Handling References for anti-Thrombopoietin antibody (ABIN116255) Images
Specificity This antibody reacts with Thrombopoietin.
Purification Affinity chromatography.
Immunogen Highly purified recombinant human TPO.

Target Details Thrombopoietin

Product Details anti-Thrombopoietin Antibody Application Details Handling References for anti-Thrombopoietin antibody (ABIN116255) Images back to top
Antigen
Alternative Name Thrombopoietin (THPO Antibody Abstract)
Background Synonyms: C-mpl ligand, MGDF, Megakaryocyte colony-stimulating factor, Megakaryocyte growth and development factor, Myeloproliferative leukemia virus oncogene ligand, THPO
Gene ID 7066
NCBI Accession NP_000451
UniProt P40225
Research Area Cancer, Cell Cycle, Hormones
Pathways JAK-STAT Signaling, Hormone Activity

Application Details

Product Details anti-Thrombopoietin Antibody Target Details Thrombopoietin Handling References for anti-Thrombopoietin antibody (ABIN116255) Images back to top
Application Notes Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofhTPO (5.00 ng/mL), a concentration of 4.2-6.3 μg/mL of this antibody is required. ELISA: (Indirect): To detect hTPO by indirect ELISA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with compatible secondary reagents, allows the detection of atleast 0.2-0.4 ng/well of recombinant hTPO. (Sandwich): To detect hTPO by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with Biotinylated Anti-Human TPO (PP1074B) as a detectionantibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hTPO. Western Blot: To detect hTPO by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hTPO is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-Thrombopoietin Antibody Target Details Thrombopoietin Application Details References for anti-Thrombopoietin antibody (ABIN116255) Images back to top
Reconstitution Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
Buffer PBS, pH 7.2 without preservatives.
Preservative Without preservative
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

References for anti-Thrombopoietin antibody (ABIN116255)

Product Details anti-Thrombopoietin Antibody Target Details Thrombopoietin Application Details Handling Images back to top
Background publications

Xie, Chan, Johnson, Starr, McCarthy, Kapur, Yoder: "Thrombopoietin promotes mixed lineage and megakaryocytic colony-forming cell growth but inhibits primitive and definitive erythropoiesis in cells isolated from early murine yolk sacs." in: Blood, Vol. 101, Issue 4, pp. 1329-35, 2003