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Solid samples can be extracted by homogenization in distilled water (dH2O) and filtered, centrifuged or, if necessary, deproteinized to remove any undissolved material. Samples should be clear and colorless with pH adjusted to 7 -8. Serum and plasma samples can be assayed directly. Cell culture media should be diluted 5-10 fold in dH2O prior to assay.
Subtract the standard values from the blank value (#4) and plot the OD or F against standard concentrations.Conversions: 1000 µM NH3 equals 1.7 mg/dL or 17 ppm.