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- Target See all Caspase 3 (CASP3) Kits
- Caspase 3 (CASP3)
- Detection Method
- Fluorometric
- Application
- Fluorescence Resonance Energy Transfer Microscopy (FRET)
- Brand
- SensoLyte®
- Characteristics
- The SensoLyte® Homogeneous AFC Capase-3/7 Assay Kit uses Ac-DEVD-AFC as the fluorogenic indicator for assaying caspase-3/7 activities. Upon caspase-3/7 cleavage, Ac-DEVD-AFC generates the AFC fluorophore which has bright blue fluorescence and can be detected at Ex/Em=380 nm/500 nm. A bi-function assay buffer in this kit is designed to lyze the cells and measure the enzyme activity at the same time. Thus, this kit can measure caspase-3/7 activity in cell culture directly in a 96-well or 384-well plate without a time-consuming cell extraction step. In case the cells are cultured in larger plates or flasks, a lysis buffer and protocol for cell lysate preparation are also conveniently included in the kit. The kit is suitable for high throughput screening of apoptosis inducers and inhibitors.
- Top Product
- Discover our top product CASP3 ELISA Kit
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- Comment
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FRET-based Assay Kit
- Restrictions
- For Research Use only
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- Handling Advice
- Protect Component A from light and moisture. Keep Components A and B away from light.
- Storage
- -20 °C
- Storage Comment
- Store Components A, B, C, and E at -20 °C. For convenience, Components D and F can be stored at 4 °C.
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The inhibitor of Ca(2+)-dependent K+ channels TRAM-34 blocks growth of hepatocellular carcinoma cells via downregulation of estrogen receptor alpha mRNA and nuclear factor-kappaB." in: Investigational new drugs, Vol. 31, Issue 2, pp. 452-7, (2013) (PubMed).
: "Glomerular cell death and inflammation with high-protein diet and diabetes." in: Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association, Vol. 28, Issue 7, pp. 1711-20, (2013) (PubMed).
: "An evolved ribosome-inactivating protein targets and kills human melanoma cells in vitro and in vivo." in: Molecular cancer, Vol. 9, pp. 28, (2010) (PubMed).
: "An active extract of Lindera obtusiloba inhibits adipogenesis via sustained Wnt signaling and exerts anti-inflammatory effects in the 3T3-L1 preadipocytes." in: The Journal of nutritional biochemistry, Vol. 21, Issue 12, pp. 1170-7, (2010) (PubMed).
: "Extracts of Lindera obtusiloba induce antifibrotic effects in hepatic stellate cells via suppression of a TGF-beta-mediated profibrotic gene expression pattern." in: The Journal of nutritional biochemistry, Vol. 20, Issue 8, pp. 597-606, (2009) (PubMed).
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The inhibitor of Ca(2+)-dependent K+ channels TRAM-34 blocks growth of hepatocellular carcinoma cells via downregulation of estrogen receptor alpha mRNA and nuclear factor-kappaB." in: Investigational new drugs, Vol. 31, Issue 2, pp. 452-7, (2013) (PubMed).
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- Target
- Caspase 3 (CASP3)
- Alternative Name
- Caspase-3 (CASP3 Products)
- Synonyms
- CPP32 Kit, CPP32B Kit, SCA-1 Kit, A830040C14Rik Kit, AC-3 Kit, Apopain Kit, CC3 Kit, Caspase-3 Kit, Lice Kit, Yama Kit, mldy Kit, xcpp32 Kit, casp3 Kit, zgc:100890 Kit, CASP-3 Kit, caspase-3 Kit, caspase 3 Kit, caspase 3 S homeolog Kit, caspase 3, apoptosis-related cysteine peptidase a Kit, caspase 3, apoptosis-related cysteine peptidase Kit, CASP3 Kit, Casp3 Kit, casp3.S Kit, casp3a Kit
- Background
- Central to the execution phase of apoptosis are the two closely related caspase-3 and caspase-7. They share common substrate specificity and structure, but differ completely in the sequence of their respective N-terminal regions. Both caspase-3 and caspase-7 have substrate selectivity for the amino acid sequence Asp-Glu-Val-Asp (DEVD).
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