SKP2 ELISA Kit

Catalog No. ABIN2044485

Product Details SKP2 ELISA Kit

Target: SKP2 (S-Phase Kinase-Associated Protein 2 (SKP2))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Minimum Detection Limit: 0.15 ng/mL

Application: ELISA

Brand: BioAssay™

Sensitivity: < 0.061 ng/mL

Application Details

Plate: Pre-coated

Protocol: S-Phase Kinase Associated Protein 2 (SKP2) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to S-Phase Kinase Associated Protein 2 (SKP2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to S-Phase Kinase Associated Protein 2 (SKP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain S-Phase Kinase Associated Protein 2 (SKP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of S-Phase Kinase Associated Protein 2 (SKP2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for SKP2

Target: SKP2 (S-Phase Kinase-Associated Protein 2 (SKP2))

Alternative Name: S-Phase Kinase Associated Protein 2 (SKP2) (SKP2 Products)

Synonyms: FBXL1 ELISA Kit, FBL1 ELISA Kit, FLB1 ELISA Kit, p45 ELISA Kit, RGD1562456 ELISA Kit, S-phase kinase-associated protein 2 (p45) ELISA Kit, S-phase kinase associated protein 2 ELISA Kit, Skp2 ELISA Kit, SKP2 ELISA Kit

Pathways: Mitotic G1-G1/S Phases