FITC Annexin V Apoptosis Detection Kit with PI

Details for Product No. ABIN2669213, Supplier: Log in to see
Antigen
  • anx
  • anx5
  • ANX V
  • anxa5
  • cb989
  • wu:fa98f06
  • wu:fj10f10
  • MGC89158
  • ANX5
  • ENX2
  • PP4
  • RPRGL3
  • Anx5
  • R74653
  • LC5
  • enx2
  • annexin A5
  • annexin A5b
  • Annexin A5
  • annexin 5
  • annexin A5-like
  • ANXA5
  • anxa5b
  • anxa5
  • LOC100220003
  • LOC100305020
  • LOC100342904
  • Anxa5
  • LOC100521982
Alternatives
Human Annexin V ELISA Kit
Reactivity
Mammalian
17
15
15
14
13
8
7
7
4
2
2
2
2
1
1
1
1
Conjugate
FITC
Application
Multiplex Assay (MA)
Options
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Characteristics FITC Annexin V Apoptosis Detection Kit with PI has been specifically designed for the identification of apoptotic and necrotic cells. Annexin V (or Annexin A5) is a member of the annexin family of intracellular proteins that binds to phosphatidylserine (PS) in a calcium-dependent manner. PS is normally only found on the intracellular leaflet of the plasma membrane in healthy cells, but during early apoptosis, membrane asymmetry is lost and PS translocates to the external leaflet. Fluorochrome-labeled Annexin V can then be used to specifically target and identify apoptotic cells. Annexin V Binding Buffer is recommended for use with Annexin V staining. Annexin V binding alone cannot differentiate between apoptotic and necrotic cells. To help distinguish between the necrotic and apoptotic cells we recommend use of our Propidium Iodide Solution (PI). Early apoptotic cells will exclude PI, while late stage apoptotic cells and necrotic cells will stain positively, due to the passage of these dyes into the nucleus where they bind to DNA. Propidium iodide is a fluorescent dye that binds to DNA. When excited by 488 nm laser light, it can be detected with in the PE/Texas Red® channel with a bandpass filter 610/10. It is commonly used in evaluation of cell viability or DNA content in cell cycle analysis by flow cytometry.
Components FITC Annexin V Apoptosis Detection Kit with PI
Alternative Name Annexin V (ANXA5 ELISA Kit Abstract)
Research Area Cardiovascular, Atherosclerosis, Apoptosis/Necrosis, Coagulation
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Precaution of Use Caution: Propidium Iodide Solution is toxigenic and mutagenic, handle with care.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store between 2°C and 8°C.
Supplier Images
Flow Cytometry (FACS) image for FITC Annexin V Apoptosis Detection Kit with PI (ABIN2669213) FITC Annexin V Apoptosis Detection Kit with PI
Flow Cytometry (FACS) image for FITC Annexin V Apoptosis Detection Kit with PI (ABIN2669213) FITC Annexin V Apoptosis Detection Kit with PI (Image 2)
Background publications Jaishy, Zhang, Chung, Riehle, Soto, Jenkins, Abel, Cowart, Van Eyk, Abel: "Lipid-induced NOX2 activation inhibits autophagic flux by impairing lysosomal enzyme activity." in: Journal of lipid research, Vol. 56, Issue 3, pp. 546-61, 2015 (PubMed).

Wang, Zhao, Yang, Bi, Chi, Tian, Li: "High-dose alcohol induces reactive oxygen species-mediated apoptosis via PKC-β/p66Shc in mouse primary cardiomyocytes." in: Biochemical and biophysical research communications, Vol. 456, Issue 2, pp. 656-61, 2014 (PubMed).