C5A ELISA Kit

Catalog No. ABIN2690553

Product Details C5A ELISA Kit

Target: C5A (C5a) (Complement Component 5a (C5a))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Minimum Detection Limit: 0.047 ng/mL

Application: ELISA

Purpose: The Human C5a ELISA Kit II is for the in vitro quantitative determination of Human C5a-desArg in human EDTA plasma, serum and other biological samples.

Brand: BD OptEIA™

Sample Type: Plasma (EDTA), Serum

Analytical Method: Quantitative

Components: Antibody Coated Wells - 2 plates of 96 breakable wells (12 strips x 8 wells per plate)
Detection Antibody - 30 mL
Standards - 4 vials, lyophilized
Enzyme Concentrate (250x) - 150 μL
Standard/Sample Diluent - 30 mL
ELISA Diluent - 12 mL
Wash Concentrate (20x) - 100 mL
TMB One-Step Substrate Reagent - 30 mL
Stop Solution - 13 mL
Plate Sealers - 4 each

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

BD OptEIA™ Human C5a ELISA Kit II

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: 1. Store kit at 2 - 8°C. Do not use kit after expiration date.
2. Before use, bring all reagents to room temperature (18 - 25°C). Immediately after use, return to proper storage conditions.
3. Lyophilized standards are stable until kit expiration date. After reconstitution, use freshly reconstituted standard within 12 hours (stored at 2 - 8°C).

References for C5A ELISA Kit (ABIN2690553)

Pfeifer, Kawahara, Hugli: "Possible mechanism for in vitro complement activation in blood and plasma samples: futhan/EDTA controls in vitro complement activation." in: Clinical chemistry, Vol. 45, Issue 8 Pt 1, pp. 1190-9, (1999) (PubMed).

Matsushita, Fujita: "Activation of the classical complement pathway by mannose-binding protein in association with a novel C1s-like serine protease." in: The Journal of experimental medicine, Vol. 176, Issue 6, pp. 1497-502, (1993) (PubMed).

Hugli, Müller-Eberhard: "Anaphylatoxins: C3a and C5a." in: Advances in immunology, Vol. 26, pp. 1-53, (1978) (PubMed).

Bokisch, Müller-Eberhard: "Anaphylatoxin inactivator of human plasma: its isolation and characterization as a carboxypeptidase." in: The Journal of clinical investigation, Vol. 49, Issue 12, pp. 2427-36, (1971) (PubMed).

Target Details for C5A

Target: C5A (C5a) (Complement Component 5a (C5a))

Alternative Name: C5a (C5a Products)

Synonyms: C5a ELISA Kit, C5b ELISA Kit, CPAMD4 ELISA Kit, Hc ELISA Kit, RGD1561905 ELISA Kit, complement C5 ELISA Kit, C5 ELISA Kit

Pathways: Complement System, Carbohydrate Homeostasis